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Citations for Millipore Sigma :
4601 - 4650 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
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bioRxiv - Cell Biology 2022Quote: ... black 96-well plates (USA Scientific 5665-5087) were coated with poly-D-lysine (50 ng/ml, Sigma, P6407) for 1-2h at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... and 0.02% (w/v) yeast extract for 15N labelling or 15NH4C1 and 13C6 D-glucose (3 g/L, Sigma) with 0.02% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin knockdown was induced in shE-cadherin cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were minced and digested for 1 hour at 37C in digestion buffer (DMEM, 5% FBS, penicillin, streptomycin, 2.5 mg/ml Collagenase D (Sigma), 0.5 mg/ml Liberase DL (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... were coated overnight with 0.1 mg/ml poly-D-lysine dissolved in phosphate buffered saline (PBS for cell culture - Sigma). Prior to dissection ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded onto 12 mm glass coverslips pre-coated with 0.1 mg/ml poly-D-lysine (P0899, Sigma) at a density of 80000 cells/cm2 ...
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bioRxiv - Microbiology 2021Quote: ... Twenty liters of water from the surface were filtered through GF/D filters (Whatman) and collected on a 0.22 µm Durapore filter (Millipore). The Durapore filter was suspended in a lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were prepared for one colour 2D d-STORM utilizing a buffer containing 100 mM MEA-HCL (Sigma, M6500), 10% glucose (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and plated on sterile 6 or 24-well plates coated with poly-D-Lysine (sterile lyophilized 135 kD; Sigma) and 1mg/ml laminin ...
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bioRxiv - Neuroscience 2021Quote: ... at a plating density of 350 cells/well in 8-well chamber slides pre-coated with 100μg/ml poly-D-lysine (#A-003-M, Sigma). CNS myelin extracts (CME ...
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bioRxiv - Microbiology 2021Quote: ... Forty liters of water from 20 cm depth were filtered through GF/D and a 0.22 µm Durapore filter (Millipore). DNA from both sample sites was extracted from the filter using a phenol-chloroform protocol (Wright et al ...
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bioRxiv - Neuroscience 2021Quote: Sorted microglia from CCI or sham injured spinal cord were cultured in 96-well plate (1.5 × 104 cells per well in 0.2mL) in poly-D-lysine-coated (Sigma-Aldrich) plates and grown in microglia culture medium (DMEM/F-12 Glutamax ...
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bioRxiv - Biochemistry 2020Quote: ... MDA-MB-231 scramble or AKAP95 knockdown cells were treated with 5 μg/ml of Actinomycin D (Millipore Sigma) or Actinomycin D together with cycloheximide for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM supplemented with BSA 0.1% for 2.5 hrs before a 10-min incubation in Krebs-Ringer Phosphate HEPES (KRPH) buffer [pH 7.4]/BSA 0.1% supplemented with a mixture of cold deoxyglucose (2-Deoxy-D-glucose; Sigma-Aldrich) and radiolabeled deoxyglucose (Deoxy-D-glucose ...
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bioRxiv - Neuroscience 2021Quote: ... the coverglasses were coated with a 10 µg/ml solution of poly-D-lysine (Sigma-Aldrich, cat. no. P6407) in double-distilled water (ddH2O ...
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bioRxiv - Neuroscience 2020Quote: ... at a density of 30,000 cells/cm2 coated with poly-D-lysine (#P6407; Sigma-Aldrich, St. Louis, MO, USA) and mouse laminin l (#3400-010-01 ...
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bioRxiv - Neuroscience 2022Quote: ... N2a NM-HAagg (EP) donor cells were treated for 3 d with methyltransferase inhibitors 5-Azacytidine (Aza) (Sigma-Aldrich), Decitabine (Dec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of 50 mM citrate buffer - pH 4.8 and 50 μL of 0.50-nitrophenyl β-D-glucopyranoside 1 mM (pNP-G Sigma) as substrate ...
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bioRxiv - Neuroscience 2022Quote: ... chambered cover glasses were pre-coated with 10 μg/ml Poly-D-lysine (PDL; Sigma Aldrich, cat. no. P6407) solution in double-distilled water (ddH2O ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Immunology 2022Quote: Decreasing concentrations of recombinant DC-SIGN or rfhSP-D (2, 1, 0.5 or 0 µg 100µl/well) were coated on polystyrene microtiter plates (Sigma-Aldrich) at 4°C overnight using carbonate/bicarbonate (CBC ...
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bioRxiv - Immunology 2022Quote: ... Cell Counting Kit-8 (CA1210) and D-Luciferin (L6882) were all purchased from Sigma-Aldrich (Saint Louis, MO, USA). Lipofectamine2000 was purchased from Invitrogen (11668019 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded in a T-75 flask previously coated with 10μg/ml poly-D-lysine (Sigma Aldrich, P7280) overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Tubes were kept at 37°C in a heating block (DB200/2; Techne) and D-biotin (B4501; Sigma-Aldrich) at a final concentration of 500 μm was added at each time point (for the majority of experiments 35 min) ...
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bioRxiv - Immunology 2020Quote: ... The tissue was then dissected into pieces and thoroughly washed with ice-cold PBS followed by digestion in 0.5 mg/ml collagenase D (Sigma), 0.5 mg/ml DNase I (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... and -WT cells were seeded per well in triplicates in a 96-well white plate coated with poly-D-lysine (Mw 75,000-150,000 Da, Sigma-Aldrich), and incubated overnight in 100 μL DMEM high glucose without phenol red (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Cell Biology 2020Quote: ... and gravity-sedimented for 3-5 min onto coverslips coated with poly-D lysine (Sigma-Aldrich, catalog number P1024), until cells became in contact with the glass ...
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bioRxiv - Microbiology 2021Quote: ... gene expression was induced by the addition of iso-propyl-β-D-thio-galactopyranoside (IPTG, Sigma-Aldrich, 0.5 mM) and plates were supplemented with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal ...
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bioRxiv - Immunology 2020Quote: ... The prepared medium was autoclaved and supplemented with a final concentration of 250 µg/mL D-cycloserine (Sigma-Aldrich), 16 µg/mL cefoxitin (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... and adipokine levels were measured via ELISA (adiponectin: Millipore EZ-MADPK, leptin: Millipore EZML-82K, resistin: R&D MRSN00). For HFD experiments ...
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bioRxiv - Physiology 2020Quote: ... Cells were plated on 100 mm tissue culture plates coated with poly-D-lysine (Sigma Aldrich, Saint Louis, MO) and grown to ~60% confluence ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells and red blood cells were excluded using 1 μg/ml 7-amino actinomycin D (7AAD; Sigma-Aldrich), Fixable Viability Dye eFluor® 780 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded on 1.5H 13 mm glass coverslips coated with poly-D-lysine 10 μg/mL (Sigma, P0899) diluted in 1X borate buffer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-DIX proteins were eluted with three column volumes of lysis buffer containing 10 mM D-(+)-maltose monohydrate (Sigma) applied to loose resin under gentle rotation for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... were expanded to 70-85% confluency in DMEM Glutamax (+ 4.5g l−1 D-Glucose, - Pyruvate) supplemented with 10% FBS (Sigma), 1% G418 (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... corneal tissues were extracted from mice and incubated in 50 μL of 2 mg/mL collagenase D (Sigma C0130) in PBS for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: HEK293fT cells were seeded at 0.1 × 106 cells/well on 12mm coverslips precoated with poly-D-lysine (Millicell EZ slide, Millipore) and cultured as described before above ...
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bioRxiv - Cell Biology 2020Quote: ... and NE-4C was cultured in dishes coated with 10 μg/ml poly-D-lysine (PDL. Sigma-Aldrich, #P0899).
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bioRxiv - Cell Biology 2021Quote: Tam-treated MBNL1Fl/Fl and MBNL1Fl/Fl-Tcf21iCre cardiac fibroblasts were infected with either AdGFP (controls) or AdMBNL1 for 36 hours at which time transcription was inhibited with 2μg/ml Actinomycin-D (Sigma). RNA was isolated from each group after 0 ...
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bioRxiv - Neuroscience 2020Quote: ... Surfaces of the Mylar film were coated by poly-D-lysine (0.1mg/ml in Trizma buffer, pH 8, Sigma) overnight ...
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bioRxiv - Physiology 2020Quote: ... Animals received a mixture of Ensure Plus containing 125mg/ml glucose + 125mg/ml D-glucose-6,6-d2 (Sigma-Aldrich) + 12.5mg/ml L-Valine-15N (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... transfected HEK293T cells on 24-well plates were detached by trypsin and re-plated onto poly-D-lysine (Sigma) -coated 25-mm glass coverslips ...
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bioRxiv - Physiology 2021Quote: ... 12.8 μg/mL ascorbic acid and 2.15 mg/mL β-glycerophosphate (D-1159, A-8960, G-9422, Sigma-Aldrich) for 13 days ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Neuroscience 2021Quote: ... the dishes were coated with poly-D-lysine (PDL) by overnight incubation with 100 μg/ml PDL (Sigma P0899). Cells were cultured at 37°C and 5% CO2 in a 2:1 mix of NB:DMEM ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at a density of 0.8 × 106 cells per well on 6-well plates pre-coated overnight with 0.1 mg/ml poly-D-lysine (Millipore) and one third of the media in each well was replaced every 3 days ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then washed once with D-PBS for 10 min and permeabilized for 30 minutes with D-PBS containing 0,2% (v/v) Triton X-100 (Sigma) and 1 % (w/v ...