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Citations for Millipore Sigma :
4401 - 4450 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 2 mM thymidine for 24 h followed by release from thymidine into fresh medium containing 100 ng/ml nocodazole (Sigma, M1404) for 18 h ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... for 1 h and for inhibition of stress granule formation cells were treated with 1.5 µg/ml of ISRIB (Sigma-Aldrich, SML0843). Stable HeLa cell lines constitutively expressing human marker proteins for membraneless compartments ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... heparin synchronised (4 h invasion window) trophozoite stage parasites were treated for one cell cycle (trophozoite to trophozoite) with GlcN (Sigma-Aldrich) and parasite pellet extensively washed in PBS containing protease inhibitor cocktail (PI ...
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bioRxiv - Molecular Biology 2022Quote: ... hCAI with the K978C mutation (h^K978C) or for T2A mutagenesis were designed using SnapGene software (SI, Table 7) and synthesised by Sigma Aldrich.
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bioRxiv - Systems Biology 2022Quote: ... The tip of the silanized pipettes was filled with a H+-selective ionophore cocktail (hydrogen ionophore I cocktail A, Sigma-Aldrich) by dipping the tip of the pipette into the solution ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates prepared from the cells at 24 h post transfection were incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich) or HaloLink resin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Developmental Biology 2024Quote: Fully grown oocytes were harvested from female mice at 28–35 days of age that were superovulated 44 h later by injection of 5 IU of PMSG in 37 °C pre-warmed M2 medium (M7167; Sigma-Aldrich) and cultured in M16 medium (M7292 ...
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bioRxiv - Developmental Biology 2024Quote: Total mRNA was extracted from larval brains of control (yw) and mrtf[Delta7] at 24 h ALH using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media from confluent 7PA2 cells or control CHO cells cultured in plain DMEM for 16 h was concentrated 10x by using Amicon Ultra Centrifugal Filter with 3kDa cutoff (Millipore UFC500324). The concentration of Aβ1-42 was determined by ELISA assay (Thermo Fisher Scientific KHB3442) ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20 x 107 HeLaK cells pre- synchronized by thymidine treatment (2 mM for 18 h) were released to undergo S-phase in presence of F-ara-Edu (5 µM; Sigma-Aldrich) for 5 h ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was cleared by centrifugation (158,000 g, 4°C, 1 h) and incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220) for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... then were incubated with the secondary antibody (Alexa-Fluor®-647-conjugated goat anti-mouse IgG H+L,1:2000 dilution) for 60 min at room temperature and counterstained with DAPI (Sigma-Aldrich) for another 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mg/mL of streptomycin (P/S) (Cat# 4-01F00-H, BioConcept) and 10 % fetal bovine serum (FBS) (Cat# S0615, Sigma-Aldrich) at 37 °C in a humidified atmosphere with 5 % CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-L-Lysine and Laminin coating of 24-well plates was performed by 48 h incubation at 37°C using Tissue Culture water (Sigma, W3500) supplemented with 1 μg/ml Poly-L-Lysine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...
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bioRxiv - Cell Biology 2023Quote: ... briggsae ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 400 μl with (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Culture supernatants were harvested 24 h post-viral infection and then passed through 0.22 μM pore-sized filters (Merck Millipore, Carrigtwohill, Ireland) to remove cell and debris and obtain viral suspension for experiments.
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bioRxiv - Plant Biology 2023Quote: Five days-old seedlings were treated for 24 h with 10 μM staurosporine (stock solution 100 mM in dimethylsulfoxide; Sigma S5921). On the sixth day ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... The solution was stirred for 10 h and then purified using a centrifugal filter (Millipore, Amicon Ultra, MWCO of 50 kDA) and freeze-dried ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (Cat#MAB-318, Millipore Sigma) at 1:1000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... Sheared chromatin equivalent to 2 × 106 cells was diluted 1:10 in ChIP Dilution Buffer and protease inhibitor cocktail and incubated for 16 h with 20 μL Magnetic Protein A/G Beads (Millipore #CS204457) and 5 μL antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... BSA in Tris buffered saline and Tween 20 (TBS-T) buffer for 1 h and incubated overnight with the following primary antibodies: Anti-LINE1 ORF1p (MABC1152) (EMD Millipore), Recombinant Anti-LINE1 ORF1p antibody [EPR21844-108] (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... Central transverse sections of the heart were cut into 4 μm sections and stained with haematoxylin and eosin (H&E) and Masson’s Trichrome staining (#HT15, Sigma-Aldrich, UK) for collagen detection ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were grown to 70–80% confluency (optionally with 1 μg/ml doxycycline for 24–48 h) in 24- or 12-well plates and treated with colchicine (10 μM, Sigma PRH1764), combretastatin A4 (Selleckchem S7783) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... the culture media of RPE-1 cells was supplemented for 24 h with 0.1 and 0.2 nM Paclitaxel (Taxol; Sigma, St. Louis, EUA) in DMSO ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of colon and spleen were stimulated for 3 h at 37°C with 50 ng/ml PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed with PBST and incubated at room temperature for 2 h with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) diluted in PBST ...
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bioRxiv - Immunology 2023Quote: ... Staining was done using hematoxylin and eosin (H&E) to view the alveolar spaces and inflammatory cells and Grocott’s Methenamine Silver stain (GMS) (Sigma-Aldrich, USA) to stain fungal cells in infected tissue ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...