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Citations for Millipore Sigma :
4301 - 4350 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Cancer Biology 2020Quote: ... washed in warm PBS and then chased for 18 h without radioactivity in DMEM containing 10% FBS and 10 mM valine (Sigma-Aldrich), to allow degradation of short-lived proteins as previously described67 ...
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bioRxiv - Neuroscience 2020Quote: ... The polymer used to form the PLGA microspheres was 50:50 lactic acid to glycolic acid with 29 kDA molecular weight (Resomer RG 503 H; Sigma-Aldrich). An oil-in-water emulsion with solvent evaporation technique as previous described 14,52,53 ...
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bioRxiv - Bioengineering 2021Quote: ... We sterilized the micropatterned molds under UV for 1 h and then treated them with 1% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 1 h at room temperature to prevent the hydrogel from sticking to the PDMS mold ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were first incubated for 1 h at room temperature in a blocking solution containing 4 % bovine serum albumin (Sigma-Aldrich) in PBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... After 10 days constructs were fixed in 4% paraformaldehyde and embedded in paraffin and stained with H&E and stained for Fibronectin with FN1 antibody (F3648, Sigma Aldrich). For each construct the number of cells invading into the collagen was counted in at least five different fields of view under light microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A594-conjugated secondary donkey anti-goat antibody (1:500, EMD Millipore). Sections were then washed in PBS and blocked with 10% normal goat serum (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... DIV10 neurons were cultured in amino acid-modified (see above for description) or standard culture medium for 48 h post-transfection and then exposed to 2 μM puromycin (Millipore, 540222) for 10 min at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... cells were either left unstimulated or activated for 4 h with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (500 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating tissue sections were washed three times for 10 minutes with PBS then incubated for 2 h in a PBS-blocking solution containing 0.5 % Saponin (Sigma Aldrich 84510) and 10 % pre-immune goat serum (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Virus supernatants were removed 7 h post-spin inoculation and cells were maintained in fresh growth medium for 48 h prior to selection in 3 μg/mL puromycin (Sigma-Aldrich). Stable cell lines were generated after 8 days of selection.
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891).
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged V737N β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891). No respiratory repression of MCF10A or MB-MDA-231 cells was observed with 200 ng/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was blocked for 1 h with 5 % milk in TBST and blotted for anti-ssDNA (1:1000, Millipore, MAB3034).
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bioRxiv - Immunology 2022Quote: ... 2 × 105 MOs were stimulated for 24 h at 37 °C and 5% CO2 with 1 μg/mL LPS (Escherichia coli 0127:B8, Sigma-Aldrich). After LPS elimination ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich) was supplemented in the culture to a final concentration of 30 μM ...
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bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transferred to black-walled 96-well plates (Falcon) 4 h before the assay and incubated with EBM or 200nM Staurosporine (Sigma Aldrich) for 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... U937 cells were differentiated to macrophages by 24 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24 h incubation in RPMI medium ...
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Pathology 2022Quote: ... The cultured C3H/10T1/2 cells were infected with 5 ml of retroviral supernatant for 48 h with 10 µg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 56 d at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... a 96-well plate was seeded with 1×104 L929 murine fibroblast cells per well and incubated for 24 h to allow for cell adhesion and spreading (L929 cell line was purchased from Sigma-Aldrich, Poznań ...
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bioRxiv - Cancer Biology 2022Quote: ... twice within 48 h and then selected with 1 μg/ml puromycin (Sangon Biotech, Shanghai, China) or 200 μg/ml G418 (Sigma–Aldrich) for 10 days ...
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bioRxiv - Cell Biology 2022Quote: ... in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated for 4 h in the capacitation medium supplemented or not with different concentrations of cycloheximide (CHX; C7698, Sigma-Aldrich) or chloramphenicol (CP ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor tissue was minced and digested at 37°C for 12 h in a dissociation buffer containing 0.012% (wt./vol.) collagenase XI (Sigma-Aldrich, C7657) and 0.012% (wt./vol. ...
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bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
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bioRxiv - Immunology 2021Quote: ... Monomers were loaded with 0.2 mg/ml peptide at 37°C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma-Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen) ...