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Citations for Millipore Sigma :
4651 - 4700 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... sWAT harvested from 5-week-old db/db mice was minced and digested with collagenase D (2.5 mg/mL, Sigma) for 30 min shaking at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos are recovered at E0.5 by dissecting in 37°C FHM (LifeGlobal, ZEHP-050 or Millipore, MR-122-D) from the oviduct the ampula ...
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Hormonal contraceptives alter amphetamine place preference and responsivity in the intact female ratbioRxiv - Animal Behavior and Cognition 2021Quote: ... Conditioning sessions were separated by two days and each consisted of an intraperitoneal (i.p.) injection of either d-amphetamine (AMP; 2.0 mg/kg, Sigma-Aldrich) or 0.9% sterile saline prior to placement within the conditioning chamber with the retractable door closed ...
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bioRxiv - Cell Biology 2020Quote: ... Trim9-/-/Trim67-/- (Trim9/Trim67 double knockout, 2KO) murine E15.5 litters were dissociated and cultured on Poly-D-Lysine (Sigma) treated dishes ...
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bioRxiv - Cell Biology 2021Quote: ... and expanded to 70-85% confluency in DMEM Glutamax (+ 4.5g/L D-Glucose, - Pyruvate) supplemented with 10% FBS (Sigma), 1% G418 (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... the γ-secretase inhibitor (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester (DAPT; R&D) or dimethyl sulfoxide (DMSO; Sigma) was added to media at the concentrations indicated.
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bioRxiv - Immunology 2020Quote: ... purified neutrophils were first incubated in a 96 wells microplate for 30 min at 37°C in RPMI complete medium with or without 2µg/well of cytochalasin D (Sigma), and then for 1 h with 20µg/wells of pHrodo E ...
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bioRxiv - Immunology 2020Quote: ... rfhSP-D was purified from the dialysate by affinity chromatography using a maltose agarose column (5 ml; Sigma-Aldrich). The bound rfhSP-D to the maltose was eluted using elution buffer (50 mM Tris–HCl ...
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bioRxiv - Immunology 2020Quote: ... Controls included cells cultured at 4°C and 37°C in the presence of 10μM cytochalasin D (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... donor splenocytes were isolated from naive B6 or IFNAR−/− mice by digesting the spleen with 2.5 mg/mL collagenase D (11088882001, Sigma) at 37°C for 30 min and labeled with 1.0 μM CFSE (eBioscience 65-0580-84 ...
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bioRxiv - Neuroscience 2021Quote: Dissociated human iPSC-derived motor neurons were re-plated on poly-D-lysine (PDL; 100 µg/ml; Sigma-Aldrich) and CSPG-(0.2 ng/ml ...
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bioRxiv - Biophysics 2022Quote: ... 1% DDM [n-Dodecyl-β-D- maltoside]) containing cOmplete mini protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Lysates were mixed 1:1 with 2X LDS sample buffer containing 50 mM TCEP (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, Cat# 1845-SH-025, R and D) and purmorphamine (1.5 μM, Cat# SML0868, Sigma) and cultured for 5 days (d14 of differentiation) ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were plated on glass coverslips (Assistent) precoated with 55 µg/ml poly-D,L-ornithine (Sigma-Aldrich) in plating medium composed of MEM supplemented with horse serum (10% v/v ...
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bioRxiv - Neuroscience 2022Quote: ... then seeded at a density of 20,000 cells/cm2 on poly-D-lysine (5 μg/ml PDL, Sigma #P6407) coated plates.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were seeded in a confocal culture dish coated with 100 µg/mL poly-D-lysine (Sigma, P0899) at a density of 2×105 cells per dish ...
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bioRxiv - Microbiology 2023Quote: ... The chromogenic substrate ortho-nitrophenyl β-D-galactopyranoside (ONPG) and chicken egg white lysozyme were purchased from Sigma-Aldrich. PopCulture® Reagent was purchased from Millipore.
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bioRxiv - Genetics 2023Quote: The siRNA screen was performed in 384-well round bottom plates coated with poly-D-lysine (Sigma-Aldrich #P7886). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were maintained in an incubator at 37ºC with 5% CO2 on 10cm cell culture dishes coated with poly-D-Lysine (Sigma).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Pre-hybridization was performed in a prehybridization solution (10 mM Tris, pH 7.5, 600 mM NaCl, 1 mM EDTA, 0.25% SDS, 1X Denhardt’s (Sigma, #D-2532), 50% formamide (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical neurons were plated at the density of 42,000/cm2 on plastic or glass slides treated with Poly-D-Lysine (100μg/ml, Sigma, P6407), and cultured in an incubator at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Mouse and rat neural tissue was dissected out in sterile ice-cold 1X-phosphate buffered saline (PBS) supplemented with 0.6% D-glucose (Sigma), under sterile conditions.
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bioRxiv - Biochemistry 2023Quote: ... were transformed with the pET28 plasmid and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Millipore Sigma) when the optical density at 600 nm reached 0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 % (v/v) n-dodecyl-β-D-maltoside (DDM from Inalco Pharmaceuticals) and 1.6 mg/mL iodoacetamide (Sigma-Aldrich) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and cultured at 37°C and 5% CO2 on poly-d-lysine (0.1 mg/ml, molecular weight <300 kDa; Sigma) and laminin (20 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... All plasticware for culturing oligodendrocyte precursors were coated with 0.01 mg/ml poly-D-lysine hydrobromide (PDL, Sigma P6407) resuspended in water ...
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bioRxiv - Neuroscience 2022Quote: ... Muscle tissues were minced into small pieces with scissors and added to a 50mL conical tube with 1mL of enzyme mix (500 U/mL type II collagenase [MP biomed], 1.5U/mL Collagenase D [Sigma] 2.5U/mL Dispase II [Sigma] ...
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bioRxiv - Molecular Biology 2022Quote: ... and replated at a density of approximately 155,000/cm2 on to plates coated with poly-D-lysine (10 µg/mL, Sigma), followed by GFR matrigel ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated on coverslips or culture dishes coated with 50 μg/ml poly-D-lysine (P6407, Sigma) and 1 μg/ml laminin (354232 ...
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bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
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bioRxiv - Molecular Biology 2024Quote: ... Nickel-3,3’-diaminobenzidine (Ni-DAB) solution (0.175M Sodium acetate, 1% Nickel ammonium sulphate, 50mg DAB (Sigma-Aldrich, D-5905)) with 0.075 % H202 was used to develop the colour for 6 min stopping the reaction by washing the sections 2 x 5 min in dH2O ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... GG-12-1.5h-pre) were prepared by coating with poly-D-lysine (0.1 mg/mL overnight, Sigma-Aldrich, P6407), poly-L-ornithine (0.01% overnight ...
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bioRxiv - Physiology 2024Quote: ... The culture medium was a 1:1 mixture of DMEM and Ham’s F-12 medium (D 8900, Sigma-Aldrich). For the concentration-response experiments ...
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bioRxiv - Neuroscience 2024Quote: ... generated by adding 25,000 glia cells on 18 mm glass coverslips coated with 0.1 mg/ml poly-d-lysine (Sigma Aldrich) in 12-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Neuroscience 2024Quote: ... 24-well plate and 96-well coated first with poly-D-lysine (70 kDa, 10 μg/ml; Sigma-Aldrich), respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were flushed from the oviduct (E3.5) or uterine horns (E4-E5.75) using 5-10 ml of warm M2 medium (MR-015-D, Sigma Aldrich) supplemented with 1 % Penicillin-Streptomycin (15130122 ...
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bioRxiv - Cell Biology 2023Quote: ... the gels were transferred to a 6 cm glass bottom dish pre-coated with 0.1% (weight/volume) Poly-D-lysine (Sigma) for imaging.
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were processed for analysis or cultured in 20uL drops of KSOM EmbryoMax medium (Sigma-Aldrich #MR-106-D) carefully covered by mineral oil suitable for embryo culture (lifeguard oil #LGUA-5 00 ...
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bioRxiv - Cancer Biology 2023Quote: ... the samples were minced into small pieces and then enzymatically digested with collagenase D (1.5 mg/ml, Sigma-Aldrich) for 45 min at 37° C in a shaking heater at 800 rpm ...
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bioRxiv - Microbiology 2023Quote: ... duplicate 120 mL water samples were sequentially filtered through a 2.7 µm GF/D filter (Whatman, UK) and 0.2 µm Sterivex filter (Millipore, USA) using a handheld 60 mL syringe (Becton-Dickinson ...
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bioRxiv - Cell Biology 2024Quote: ... The double stranded RNA expression was induced by adding 0.4 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) (I6758, Sigma) and its concentration was further increased to 0.8 mM prior to seeding the plates ...
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bioRxiv - Neuroscience 2023Quote: Primary astrocytes at passage 5 were seeded at the density of 10,000–12,000 cells on poly-D-lysine (Merk/Sigma-Aldrich)-coated glass coverslips (12 mm in diameter ...
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bioRxiv - Pathology 2023Quote: ... Livers were removed and incubated for 30 min at 37°C under gentle agitation with HBSS containing collagenase D (0.2 mU/mL, Sigma). Cell suspensions were passed through a 100 μm cell strainer before staining ...
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bioRxiv - Immunology 2023Quote: ... TFPlo cells were sorted and incubated in T cell media + 55µM BME containing 1:1 anti CD3/CD28 Dynabeads in a 96 well round bottom plate with either 5µg/mL Actinomycin D (Sigma) in DMSO or DMSO alone (vehicle ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek) coated with poly-D-lysine (Sigma Aldrich). The following day ...