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Citations for Millipore Sigma :
4701 - 4750 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Nickel-3,3’-diaminobenzidine (Ni-DAB) solution (0.175M Sodium acetate, 1% Nickel ammonium sulphate, 50mg DAB (Sigma-Aldrich, D-5905)) with 0.075 % H202 was used to develop the colour for 6 min stopping the reaction by washing the sections 2 x 5 min in dH2O ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were flushed from the oviduct (E3.5) or uterine horns (E4-E5.75) using 5-10 ml of warm M2 medium (MR-015-D, Sigma Aldrich) supplemented with 1 % Penicillin-Streptomycin (15130122 ...
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bioRxiv - Bioengineering 2023Quote: ... GG-12-1.5h-pre) were prepared by coating with poly-D-lysine (0.1 mg/mL overnight, Sigma-Aldrich, P6407), poly-L-ornithine (0.01% overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek) coated with poly-D-lysine (Sigma Aldrich). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... The double stranded RNA expression was induced by adding 0.4 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) (I6758, Sigma) and its concentration was further increased to 0.8 mM prior to seeding the plates ...
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bioRxiv - Biophysics 2022Quote: ... 1% DDM [n-Dodecyl-β-D- maltoside]) containing cOmplete mini protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Lysates were mixed 1:1 with 2X LDS sample buffer containing 50 mM TCEP (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, Cat# 1845-SH-025, R and D) and purmorphamine (1.5 μM, Cat# SML0868, Sigma) and cultured for 5 days (d14 of differentiation) ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were plated on glass coverslips (Assistent) precoated with 55 µg/ml poly-D,L-ornithine (Sigma-Aldrich) in plating medium composed of MEM supplemented with horse serum (10% v/v ...
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bioRxiv - Neuroscience 2022Quote: ... then seeded at a density of 20,000 cells/cm2 on poly-D-lysine (5 μg/ml PDL, Sigma #P6407) coated plates.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were seeded in a confocal culture dish coated with 100 µg/mL poly-D-lysine (Sigma, P0899) at a density of 2×105 cells per dish ...
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bioRxiv - Microbiology 2023Quote: ... The chromogenic substrate ortho-nitrophenyl β-D-galactopyranoside (ONPG) and chicken egg white lysozyme were purchased from Sigma-Aldrich. PopCulture® Reagent was purchased from Millipore.
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Mouse and rat neural tissue was dissected out in sterile ice-cold 1X-phosphate buffered saline (PBS) supplemented with 0.6% D-glucose (Sigma), under sterile conditions.
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bioRxiv - Neuroscience 2023Quote: ... All cells were maintained in an incubator at 37ºC with 5% CO2 on 10cm cell culture dishes coated with poly-D-Lysine (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Cortical neurons were plated at the density of 42,000/cm2 on plastic or glass slides treated with Poly-D-Lysine (100μg/ml, Sigma, P6407), and cultured in an incubator at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The cleaned embryo is then placed on a 3.5cm petri dish containing 2ml of culture gel made with the following formula (per 100ml of culture gel): Part A: 50ml Albumin (beaten for 15min) then supplement with 0.8ml 20% D-Glucose (Sigma); Part B ...
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bioRxiv - Immunology 2023Quote: ... Culturing and induction of protein expression following application of the inductor isopropyl-β-D-thiogalactopyranoside (IPTG; Sigma, Deisenhofen, Germany)) was performed as previously described (33).
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bioRxiv - Developmental Biology 2023Quote: ... were assembled and prepared as per the manufacturer’s instruction with the cover glass coated overnight with 500μg/ml poly-D-lysine (Sigma-Aldrich). Once assembled ...
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bioRxiv - Developmental Biology 2023Quote: ... zona pellucidae (ZP) were removed from E3.5 blastocysts by brief treatment with acidic Tyrode’s solution (Millipore MR-004-D). After removing zona pellucidae ...
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bioRxiv - Molecular Biology 2023Quote: ... Fresh oleic or palmitoleic acid from a -20°C stock was dissolved in 500 μL DMSO (Sigma, D- 8418) then added into autoclaved warm medium to reach the final concentration ...
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bioRxiv - Genomics 2023Quote: ... EBs were dissociated with trypsin and neural progenitor cells (NPCs) were plated on poly-D-lysine hydrobromide (Sigma Aldrich) and laminin (Roche ...
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bioRxiv - Neuroscience 2023Quote: Transduced CHO-K1 cells expressing hα7-nAChR and chaperone proteins were cultured on 50µg/ml poly-D-lysine (Sigma-Aldrich) and 330µg/ml Collagen type 1 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... They were then added to 2 mL of digestion buffer (RPMI containing Collagenase D (2mg/mL; Sigma-Aldrich; 11088882001), Collagenase 11 (2mg/mL ...
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bioRxiv - Immunology 2023Quote: ... disrupted (between 2 frosted glass slides) and digested with 1mg/ml of collagenase D (Sigma Life Science; ref # 11088882001) and 100µg/ml of DNAse I (Sigma Life Science ...
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bioRxiv - Microbiology 2023Quote: ... D-alginate was produced by enzymatically degrading 2% alginate with 1 unit ml-1 of alginate lyase (Sigma Aldrich) at 37 °C for 24 hours ...
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bioRxiv - Genetics 2023Quote: The siRNA screen was performed in 384-well round bottom plates coated with poly-D-lysine (Sigma-Aldrich #P7886). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... were transformed with the pET28 plasmid and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Millipore Sigma) when the optical density at 600 nm reached 0.6 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Pre-hybridization was performed in a prehybridization solution (10 mM Tris, pH 7.5, 600 mM NaCl, 1 mM EDTA, 0.25% SDS, 1X Denhardt’s (Sigma, #D-2532), 50% formamide (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 % (v/v) n-dodecyl-β-D-maltoside (DDM from Inalco Pharmaceuticals) and 1.6 mg/mL iodoacetamide (Sigma-Aldrich) for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... the samples were minced into small pieces and then enzymatically digested with collagenase D (1.5 mg/ml, Sigma-Aldrich) for 45 min at 37° C in a shaking heater at 800 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... Three days before recording non-embedded organoids were placed into CytoView MEA 48-Black (Axion, cat.no. M768-tMEA-48B-5) plate precoated with 0.1 mg/ml poly-D-lysine hydrobromide (Sigma, cat.no.P7886) for 24 hours following 10 µg/ml laminin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... the macrosections were optically cleared by incubation in a series of 10 mL D-fructose (Millipore Sigma, Cat#: F0127) solutions at varying concentrations (20% ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred to tubes containing digestion buffer (0.05% Collagenase D Sigma-Aldrich Cat. C0130-500MG; 0.1µM TLCK, Sigma-Aldrich Cat ...
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bioRxiv - Biophysics 2024Quote: ... The resin was washed with 25 column volumes of KBT solution (150 mM KCl, 50 mM Tris, 10% glycerol, pH 7.9) and eluted with 10 mM d-Desthiobiotin (Sigma) in KBT buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
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bioRxiv - Neuroscience 2024Quote: ... were placed in the center of wells from 96-or 24-well plates coated with 0.1mg/mL poly-D-lysine (Sigma) and 3mg/mL laminin (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... Reverse transcription (RT) was performed by the addition of 75μl RT reagents: 50ng/μl actinomycin D (Sigma-Aldrich, USA), 0.5mM dNTPs (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 25,000 cells were plated onto 12 mm glass coverslips (Carolina Biological) coated with poly-D-lysine (>300,000 MW; Sigma). Explants and isolated neurons were incubated in Complete NBM with 100 μg/mL GDNF ...
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bioRxiv - Cell Biology 2024Quote: Huh7 cells (5 x 104 cells) were treated with 5 μg/ml of actinomycin D in DMSO (Sigma-Aldrich). Samples were harvested at the indicated times for total RNA extraction using the NucleoSpin RNA kit (Macherey Nagel ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice in FDA buffer (50 mM HEPES, pH 7.0 and 0.5 mM 2-deoxy-D-glucose; Sigma-Aldrich) followed by quantification in a CASY cell counter ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were plated at a density of ∼3.0-3.5× 105 cells/well in poly-D-lysine (P6407, Sigma-Aldrich) –coated 6-well plate and at a density of 2.5–3.0×104 cells/well in 24-well plate ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of both strains were then transferred to Brock medium G/T/U supplemented with D(+)-Glucose (Sigma Aldrich) instead of Sucrose at the same final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... 1000-2000 neurons/well were plated on pre-grown micro-islands generated by plating 6000 rat glia on 18 mm glass coverslips coated with agarose and stamped with a solution of 0.1 mg/ml poly-D-lysine (Sigma) and 0.7 mg/ml rat tail collagen (BD Biosciences)(Mennerick et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Liver biopsies were minced into small pieces and digested by incubation with 2.5 mg/ml Collagenase D (Sigma, 11088866001) in Hanks’ Balanced Salt Solution (details ...
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bioRxiv - Molecular Biology 2024Quote: ... for 24 hours prior to culture in Dulbecco’s modified essential medium (DMEM) supplemented with 5mM D-glucose (Sigma, #G7021) for 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells then removed by incubation with pre-warmed extraction buffer (20mM NH4OH, 0.5% Triton X-100 in D-PBS (Sigma)) ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Cell Biology 2024Quote: ... LonP1 loxP/loxP mice and MIP1-CreERT mice were administered 100 mg/kg/d tamoxifen (TM, T5648, Sigma-Aldrich) dissolved in filtered sunflower seed oil (Sigma-Aldrich ...