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Citations for Millipore Sigma :
4551 - 4600 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Plates were incubated at 37 °C and 5% CO2 for 72 h in a humidity chamber until resazurin (#R17017, Sigma Aldrich) dissolved in PBS was added to a final concentration of 0.01 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% (wt/vol) nonfat milk in PBST for 1 h at room temperature and probed with primary antibodies against Flag (Sigma, F1804), CST 12364S ...
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bioRxiv - Cell Biology 2024Quote: ... Viral supernatants were filtered and used to infect cells by spin-infection (2500 rpm centrifugation at 25 °C for 1 h) in the presence of 4 µg/mL polybrene (Cat#S2667, Sigma-Aldrich). Transduced cells were enriched either by antibiotic selection or expression of fluorescent protein using a BD FACSAria Fusion cell sorter.
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin) and incubated for a further 48 h in 1:100 fluorescein isothiocyanate (FITC)-labelled anti-rabbit secondary antiserum (Sigma Aldrich). A final overnight incubation in 1:100 TRITC-labelled phalloidin (200 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... On day 9 medium was supplemented with FGF8b (100 ng/ml, R&D) and heparin (1 μg/ml, Sigma). On day 11 cells were lifted using Accutase (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Immunology 2021Quote: ... lungs were cut into small pieces and incubated at 37°C in RPMI containing Collagenase D (2mg/ml, Sigma) and DNAse (50ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... while the pellet containing F-actin was resuspended in cold distilled water with 1 mM cytochalasin D (Sigma-Aldrich) and kept on ice for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... for lentiviral packaging were expanded to 70-85 % confluency in DMEM Glutamax (+ 4.5 g/L D-Glucose, - Pyruvate) supplemented with 10 % FBS (Sigma), 1 % G418 (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... BMDMs were pretreated for 30 min with hexokinase inhibitor 2-deoxy-D-glucose (2-DG; 10 mM, Sigma-Aldrich), actin polymerization inhibitor cytochalasin D (10 μM ...
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bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bands were visualized by ethidium bromide staining and the rRNA band was excised and eluted using the D-Tube TM Dialyzers according to the manufacturer’s protocol for electro elution (Novagen). Eluted rRNA fragment was digested to nucleosides using P1 nuclease and alkaline phosphatase as described before (46) ...
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bioRxiv - Microbiology 2021Quote: ... T84 and IPEC-1 cells were incubated in DMEM/F12 + 10% FBS + 2 µg/ml Cytochalasin D (Sigma-Aldrich) at 37 ℃ for 1 h to block actin-dependent invasion ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the cells were seeded at a density of 1.2 × 106 cells/ml in culture dishes coated with 20 μg/ml poly-D-lysine (Sigma). The neurons were cultured at 37°C in a humidified incubator with 5% CO2/95% air ...
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bioRxiv - Developmental Biology 2021Quote: ... Mixed cerebellar cultures (no separation step) were plated at 2.8 x 106 cells/ml on poly-D-lysine (Millipore) coated glass coverslips (Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... and then plated into 24-well (1.8 x 10 5 cells per well) plates coated with 50 μg/ml poly-d-lysine (Sigma). After removal of the coating solution ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed at the indicated time-points with 2% Paraformaldehyde (PFA/ Science Services) and 2.5% Glutaraldehyde (GA/ Science Services GmbH) in Dulbecco’s phosphate-buffered saline (D-PBS, Sigma-Aldrich) for 5 minutes at room temperature (RT ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 41 μm nylon mesh and seeded onto poly-D-lysine-coated (PDL) (Sigma-Aldrich, MO, USA) plates as needed ...
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bioRxiv - Neuroscience 2021Quote: Retinal lesions were induced by a single intravitreal injection of 0.5 - 1 nL of freshly prepared 100 mM N-Methyl-D-aspartic acid (NMDA, M3262, Sigma). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... The transformed cells at 25°C (OD600 = 0.6) were induced with 0.5 mM isopropyl b-D-thiogalactopyranoside (IPTG; Sigma, USA), which resulted in the expression of the maltose-binding protein (MBP)-tagged NlGr7 protein ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... for lentiviral packaging were expanded to 70-85% confluency in DMEM Glutamax (+4.5 g/L D-glucose, - pyruvate) supplemented with 10% FBS (Sigma), 1% G418 (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... Unhooking of RUSH-TfR from the ER was allowed by the addition of D-biotin (Sigma-Aldrich, B4501-500MG) at 100 μM final concentration in DMEM for 30 min prior fixation ...
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bioRxiv - Cell Biology 2022Quote: ... Bacteria were spotted on NGM plates containing additionally 1 mM isoprophyl-β-D-thiogalactoside (IPTG) (Sigma-Alrich, MO, usa). Incubation with dsRNA initiated 64 hours after population synchronization and then young adult worms transferring to the respective treatment plates ...
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bioRxiv - Microbiology 2020Quote: ... in M9 minimal medium supplemented with 0.4% D-glucose and 10 mM para-chloro-phenylalanine (p-Cl-Phe; Sigma) for B ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were fasted for 6 hours prior to IP injection of 2 g/kg 20% D-glucose (Sigma-Aldrich) in water with a 26G needle ...
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bioRxiv - Cell Biology 2021Quote: ... followed by measuring β-galactosidase activity measurement using 2-Nitrophenyl β-D-galactopyranoside (Sigma, St. Louis, MO, cat #N1127) as substrate.
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bioRxiv - Neuroscience 2020Quote: ... U251 cells were plated on poly-d-lysine-coated coverslips (via submersion in 0.05 mg/mL solution, Sigma-Aldrich) at 10% confluence 24 hours prior to transfection ...
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bioRxiv - Bioengineering 2021Quote: ... and 4-arm 10,000 kDa PEGDA crosslinker (Creative PEGWorks) were all reconstituted in 0.1% photoinitiatior (Irgacure D-2959, Sigma Aldrich) at 1% w/v ...
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bioRxiv - Biochemistry 2020Quote: ... L-aspartic-15N acid (98 atom% 15N) and (D3)-L-methionine (98 atom% D) were obtained from Sigma-Aldrich. Isotopically labeled 13C6-glucose (≥99 atom% 13C ...
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bioRxiv - Neuroscience 2020Quote: ... Cortices from two pups were platted in a 75cm2 flask pre-treated with Poly-D-Lys (P7280, Sigma Aldrich) and cultured at 37°C in a humidified 5% CO2 incubator ...
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bioRxiv - Neuroscience 2021Quote: ... then dissociated into single cells by stem cell acctuse (STEM CELL) and seeded in glass cover slips coated by 0.0125 mg/mL poly-D-lysine (PDL, Sigma) at the density of 50,000 cells/mL in DMEM/F12 medium containing 1% FBS (Lonsera ...
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bioRxiv - Neuroscience 2020Quote: ... fish were first anesthetized with a 0.03% solution of MS222 (pH adjusted to 7.4 with NaHCO3) and later transferred to external solution containing d-tubocurarine (10 μM, Sigma). The external solution (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... The dissociated DRG neurons were then plated onto poly-D-lysine coated 12 mm cover glass (Sigma-Aldrich, Australia). Neurons were left to attach for ~3 hours at 37°C after which media was added and then incubated overnight and used within 24 hrs.
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Cell Biology 2021Quote: ... murine CD4+ T cells were isolated and activated in a 12 well plate with culture media containing 2g/L D-Glucose-1,2-13C2 (Sigma) for 24 hours ...
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bioRxiv - Plant Biology 2021Quote: ... Separation was performed using a reversed phase column (25 cm L x 10 mm D, 5 µm, Sigma-Aldrich) controlled by the LabSolutions software ...
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bioRxiv - Plant Biology 2020Quote: ... GSTU7-His expression was induced by incubating the cells for 24 h with isopropyl ß-D-1-thiogalactopyranoside (IPTG) in a final concentration of 0.5 mM and 200 μL of anti-foaming solution (Sigma), with constant shaking at 22°C ...
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bioRxiv - Systems Biology 2021Quote: ... once with 500 μL of 1x BMg/THS/EP buffer (1x BMg/THS buffer supplemented with 20 mM EGTA (pH 8.0) and 10 μg/mL poly d(IC) (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... and digested in digest buffer (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin [Invitrogen], 1mg/mL collagenase D [Sigma] ...
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bioRxiv - Plant Biology 2021Quote: The bacterial strains used were pre-grown for 7 d in Tryptic Soy Broth 50 % (TSB 50 %, Sigma-Aldrich), and washed with 10 mM MgCl2 (series of centrifugations and removal of supernatant ...
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bioRxiv - Immunology 2021Quote: ... HCMV titers were determined by counting GFP+ foci on HFF 2 d post infection using a 1.2% methylcellulose (Sigma) overlay ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were seeded onto cell culture dishes (Falcon) or nitric-acid washed glass coverslips (Carl Roth GmbH) coated with 0,5mg/ml poly-D-lysine (Sigma) in plating medium (Basal medium Eagle’s containing Eagle’s salts ...
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bioRxiv - Microbiology 2020Quote: ... medium was replaced by the D-MEM high glucose medium with 1.5% of carboxy-methyl cellulose (CMC-Sigma Co.), 1% FBS and 1% penicillin / streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated by adding a non lysing formulation of 0.5 mM D-Luciferin substrate (23) (Sigma-Aldrich) and measuring luciferase activity for 1 second on a plate Reader Infinite 200 PRO (Tecan®) ...
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bioRxiv - Neuroscience 2021Quote: ... and seeded on Xona silicon device (#RD450) coupled with a 35mm dish previously coated with poly-D-lysine (Sigma). Cells were maintained in a 37 °C incubator with 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown in a 1-L Braun Biostat fermenter supplemented with 15NH4Cl and D-[13C] glucose (Sigma-Aldrich) as the sole source of nitrogen and carbon for the bacterial cells ...
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bioRxiv - Biophysics 2021Quote: ... Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA) and elution with 2.5mM d-desthiobiotin (Sigma). A final purification step was performed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... in DMEM solution for two 45 minute periods and transferred to 0.5 mg/mL Collagenase D in DMEM solution supplemented with 3% Liberase TL (Sigma) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and NMDA receptor mediated PSCs were blocked with DL-2-amino-5-phosphonopentanoic acid (D-AP5; 50 μM; Sigma). The AMPA-R PSC frequency and amplitude was determined from a 1 min interval after the recording had stabilized (~10 min after wash-in of picrotoxin/D-AP5) ...