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Citations for Millipore Sigma :
4251 - 4300 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to ∼2 mL with an Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). The HIS6-SUMO-tag was cleaved by proteolysis with 1 mg TEV protease for 2 hours at 4°C in the cold room ...
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bioRxiv - Biochemistry 2023Quote: 3G124.ex# and Off.ex# DARPins were concentrated to 10 mg/mL in TBS using Amicon Ultra-4 10K centrifugal filters (Millipore). Sitting drops were dispensed on Intelli-plates 96-3 LVR (Hampton Research ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess reagents were removed by MeOH/CHCl3 protein precipitation or concentration-dilution using a 0.5 mL Centrifugal Filter (3K, Millipore). Pellets were washed by 500 μL MeOH/H2O (9:1 ...
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bioRxiv - Developmental Biology 2023Quote: ... microinjection solutions containing contained 100 μM of final morpholino concentration were heated up to 75°C for 5 min and then passed through a 0.22 μm PVDF micro-filter (Millipore) placed in 500 μl tube by centrifugation at 2500 g for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... approximately 8 L of seawater was filtered onto 0.22-μm Sterivex-GP pressure filter units (Millipore, Billerica, MA, USA). The filters were immediately soaked in 2 mL of RNAlater Stabilization Solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was added to Fe-NTA agarose beads (PureCube) on a multiscreenHTS-HV 0.45 μm 96 well filter plate (Merck Millipore). After loading ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultivation medium was first clarified by centrifugation for 5 min at 1500 x g and subsequent filtering through syringe mounted PVDF filter with 0.45 µm pore size (Merck Millipore). VLPs were pelleted through a 20% sucrose cushion at 125,000 x g for 120 min ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... after which the supernatant containing pseudo-typed lentivirus particles was harvested and filtered through a 0.45-µm filter (Millipore). The viral particles were concentrated by centrifugation at 3000 x g overnight at 4°C and the pellet was resuspended in DMEM ...
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bioRxiv - Biochemistry 2023Quote: We measured TEER in of RPE plated on transwell filter membranes using an Epithelial Volt- Ohm Meter (Millipore, #MERS00002) and ERS Probes (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were buffer exchanged into 1x PBS (pH8.0) using Amicon Ultra-15 Centrifugal Filter Unit (10 kDa) (Millipore) and flash-frozen.
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Neuroscience 2024Quote: ... the transfection medium was replaced with 500 µl of the warmed conditioned medium that was sterilized by passing through a 0.2 µm filter (Millipore-Sigma). Transfected neurons were then placed in a humidified 37°C incubator until DIV 21-25 ...
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bioRxiv - Biophysics 2024Quote: ... Fractions were collected and pooled and concentrated to ∼10 mg/ml using a 30-kDa spin filter column (Millipore) before being stored at −80°C.
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bioRxiv - Biophysics 2024Quote: Purification of the folded DNA origami nanoactuators from excess staple strands was achieved using ultrafiltration with 0.5 mL Amicon centrifugal filter units with 100 kDa molecular weight cut-off (Merck Millipore). The filter units were pre-washed with buffer (1x TAE ...
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bioRxiv - Biophysics 2024Quote: ... Fractions with GPCR-containing nanodiscs were pooled and concentrated in 50 kDa Amicon-Ultra 0.5 mL centrifugal filters (Merck Millipore) to reach a final concentration of ∼60 μM ...
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bioRxiv - Biophysics 2024Quote: ... The stock solution was filtered with a Millex polyethersulfone syringe filter (0.22 μm pore size; Millipore Sigma catalog # SLGPR33RS) and stored at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted TLR3/minibinder complexes were concentrated to 1 mg/mL using an Amicon Ultra centrifugal filter (#UFC8030, Millipore) and further purified by SEC using a Superdex 200 Increase 10/300 GL column (#GE28-9909-44 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant containing lentivirus particles was harvested at 72 hours after transfection and filtered through Millex-GP Filter Unit (0.45 μm pore size, Millipore). To obtain high virus titer ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Microbiology 2024Quote: ... CHIKV nsP1 protein was concentrated to the required concentration with Amicon centrifugal filters (10,000 MWCO Millipore, Burlington, MA, USA), flash-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was harvested after 24 and 48 h and filtered through a 0.22 µm syringe filter (Millipore). To infect primary myoblasts ...
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bioRxiv - Microbiology 2024Quote: ... Peak fractions were concentrated to 1.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The flow-through containing SHPRH was collected and subsequently concentrated using an Amicon 100,000 MWCO centrifugal filter unit (Millipore). The concentrated sample was applied to a Superose 6 Increase 10/300 GL (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: ... was centrifugally filtered at 9,100g for 4 h at 4 °C through a 5-kDa cut-off filter (Millipore) to remove proteins ...
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bioRxiv - Microbiology 2024Quote: ... A 60 µl aliquot of this mating mixture was spotted onto a 0.45 µm pore mixed cellulose ester filter disc (Millipore) placed on solid LB containing DAP and incubated at 37 °C for 2 h.
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bioRxiv - Molecular Biology 2024Quote: ... The fractions that contained the SsCBE2-C2 protein were collected and concentrated through an Amicon Ultra centrifugal filter (Millipore). Following that ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the expressed protein were combined and concentrated to 2.5 mL with a 30-kDa cut-off centrifugal filter (Millipore), run through the desalting column again ...
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bioRxiv - Systems Biology 2024Quote: ... cultures were spun down at 3,000 x g for 20 min and sterile-filtered using Steriflip 0.2-μM filters (Millipore-Sigma). Media control (mixed carbohydrates media ...
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bioRxiv - Systems Biology 2024Quote: ... the virus containing supernatant was centrifuged 500 x g for 5min and filtered by using 0.45um PES filters (Millipore). The lentivirus was concentrated by using Lenti-X concentrator (Clontec) ...
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bioRxiv - Systems Biology 2024Quote: ... cultures were spun down aerobically at 3,000 x g for 20 min and sterile filtered using Steriflip 0.2-μM filters (Millipore-Sigma) before returning to the anaerobic chamber.
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bioRxiv - Genomics 2024Quote: ... centrifugation of the sample in a 100kD Amicon centrifugal filter was used (Sigma Aldrich St. Louis and Burlington, MA), and the viral particles were stored at −80 °C.
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bioRxiv - Synthetic Biology 2024Quote: ... PTP1B was recovered from glycerol stocks by buffer exchanging into 1× PBS pH 7.4 using Amicon Ultra-0.5 centrifugal filter units (10 kDa MW cut-off; Millipore Sigma). PTP1B stocks were stored exclusively at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Biotinylated PTP1B was recovered from glycerol stocks by buffer exchanging into 1× PBS pH 7.4 using Amicon Ultra-0.5 centrifugal filter units (10 kDa MW cut-off; Millipore Sigma). Biotinylated PTP1B stocks were stored exclusively at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested by filtration using a Kontes 90 mm filtration apparatus with 0.45 μm nitrocellulose filters (Millipore, HAWP09000). During the filtration ...
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bioRxiv - Biochemistry 2024Quote: ... Proteoliposomes (100 μL) were loaded into the wells of a MultiScreenHTS + HA 96-well filter plate (pore size 0.45-μm, Millipore). Uptake of the radiolabelled substrate was initiated by the addition of 100 μL buffer containing 2.5 μM [14C]-malate (American Radiolabelled Chemicals) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein digestion was performed using a modified solid-phase-enhanced sample preparation (SP3) based method adapted to a 96 well format in 0.45µM filter plates (Millipore, MSRPN04)50,51 ...
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bioRxiv - Biochemistry 2024Quote: ... Each gel slice was diced into smaller pieces and then transferred to a 96-well filter microplate (Millipore, MABVN1250) fitted into to a 96-well plate (MaxiSorpTM Nunc ...
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bioRxiv - Neuroscience 2024Quote: ... and desalted and concentrated by centrifugation at 5,000 g for 30 min at 20 °C in a prerinsed Amicon Ultra-15 filter (Millipore). AAVs were aliquoted and stored at -80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant containing lentivirus was collected 48 and 72 hr post-transfection and filtered through a 0.45-μm PVDF membrane filter (Millipore). To infect cells ...
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bioRxiv - Neuroscience 2024Quote: ... or 150 mg (samples from day 27-200) of protein was transferred to an Amicon Ultra-0.5 Centrifugal filter with a 10 kDa cutoff (Merck Millipore) and each sample was diluted to 1% SDC using a 10 mM ammonium acetate solution (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... The saturated donor and recipient cultures (100 µl each) were combined in 10 ml BHI and concentrated onto a 0.45-µm membrane filter disc (Millipore-Sigma) using vacuum filtration ...
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bioRxiv - Microbiology 2024Quote: ... The eluted solution was desalted and concentrated with phosphate buffered saline (PBS) in an Amicon Ultra centrifugal filter device with a 50,000 molecular weight cutoff (Millipore). The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... we centrifuged the organic waste samples at 5,000xg for 10 minutes followed by filtering with a 0.45 µm PVDF syringe filter (Merck Millipore, Germany). Seven Erwinia and Pseudomonas strains previously isolated from the wheat phyllosphere were used as bacterial hosts15 ...
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bioRxiv - Neuroscience 2024Quote: ... the solution was centrifuged four times at 10,000 ×g for 15 minutes each in Amicon Ultra-0.5 Centrifugal Filters (Millipore, UFC501096), with the addition of PBS to the solution remaining above the filter at each step ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated for 5 min and purified for 5 min at 5000 rcf in centrifugal filter units (PVDF membrane with 0.22 μm pore size; Millipore). A slowly tapering glass pipette was pulled from a borosilicate glass capillary (borosilicate glass capillaries ...
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bioRxiv - Microbiology 2024Quote: ... supernatants were concentrated down to 3 ml using Amicon® Ultra-15 Centrifugal Filter Unit (50 kDa cutoff, Millipore) centrifuging at 3,500 x g for 10 min at 4°C each time ...