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Citations for Millipore Sigma :
4151 - 4200 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated and buffer exchanged into 1x PBS or 1x TBS (20 mM Tris, 150 mM NaCl, pH 8.0) using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa) (Millipore). Purified proteins were snap frozen and stored at – 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg of soluble proteins in TEAB buffer were added onto 30 kDa cutoff filter (Sigma-Aldrich, cat#MRCF0R030) and centrifuged at 11,000 g for 15 minutes at RT ...
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bioRxiv - Plant Biology 2023Quote: ... production we centrifuged YM-grown bacteria at 8,220 × g for 20 min and filtered the supernatant through 0.22 μm cellulose filters (Millipore, USA). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... filtered through 0.45 μm sterilization filters and added to HC11 cells together with 8 μg/mL polybrene (Sigma-Aldrich). Transduction efficiency was monitored by checking the expression of green fluorescent protein (GFP) ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Microbiology 2023Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50 kDa cut-off (Millipore-Sigma) and quantified using a NanoDrop spectrophotometer (280 nm) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 4% SDS and 0.1 M DTE) and buffer exchange was performed in Amicon Ultra Centrifugal filter devices (0.5 mL, 30 kDa MWCO; Merck Millipore) at 14,000 rcf for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 μL ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... after which the supernatant containing pseudo-typed lentivirus particles was harvested and filtered through a 0.45-µm filter (Millipore). The viral particles were concentrated by centrifugation at 3000 x g overnight at 4°C and the pellet was resuspended in DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered with a Spin-X centrifuge tube filter (Costar) with a pore size of 0.22 µM and concentrated using a centrifugal spin concentrator (Millipore) with an MWCO of 10 kDa ...
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bioRxiv - Microbiology 2023Quote: ... were filtered using vacuum filtration (ca. -200 mm Hg) onto 47 mm diameter 0.2 µm hydrophilic Durapore filters (Millipore). Samples were immediately flash-frozen and stored in liquid nitrogen (-196 °C ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to ∼2 mL with an Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). The HIS6-SUMO-tag was cleaved by proteolysis with 1 mg TEV protease for 2 hours at 4°C in the cold room ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to 0.5 mL with a Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Biophysics 2023Quote: ... The media was centrifuged for 5 minutes at 500 g and filtered through a 0.45 μm syringe filter (Millipore). The media was concentrated with a UV-sterilized Amicon ultra-15 100 kDa centrifugal filter until the solution was concentrated by 30 times its original volume ...
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bioRxiv - Biochemistry 2023Quote: 3G124.ex# and Off.ex# DARPins were concentrated to 10 mg/mL in TBS using Amicon Ultra-4 10K centrifugal filters (Millipore). Sitting drops were dispensed on Intelli-plates 96-3 LVR (Hampton Research ...
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bioRxiv - Biochemistry 2023Quote: ... After overnight incubation at 4 °C the BAPc-MNBs were extruded through a sterile 0.22 μM syringe filter (Millex-GS, Millipore-Sigma). This sterilizes the BAPc-MNBs and excludes any large aggregated BAPc-MNBs ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... microinjection solutions containing contained 100 μM of final morpholino concentration were heated up to 75°C for 5 min and then passed through a 0.22 μm PVDF micro-filter (Millipore) placed in 500 μl tube by centrifugation at 2500 g for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Immunology 2023Quote: ... The eluted solution was desalted and concentrated with preservative buffer (PBS) in an Amicon Ultra centrifugal filter device with a 50,000 molecular weight cutoff (Millipore). The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated and buffer exchanged into 1x TBS (20 mM Tris, 150 mM NaCl, pH 8.0) using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa) (Millipore). Purified proteins were snap frozen and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using centrifugal filter units (Amicon, 10KDa cut-off; Millipore, Ultra-4 UFC801024 and/or Ultra-0.5 UFC501024) ...
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bioRxiv - Microbiology 2023Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3 kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Cancer Biology 2023Quote: ... the antibody-linker solution was washed once using a 30 kDa Amicon Ultra-2 centrifugal filter (UFC203024, Sigma-Aldrich). Annealed DNA oligonucleotides with a 5′ azide modification (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified by centrifugation (8000 X g for 2 min) and sterile-filtered using a 0.45 µm SFCA syringe filter (Millipore). Phage lysates were stored at 4°C.
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bioRxiv - Microbiology 2023Quote: The first morning urine from five healthy volunteers was pooled and filtered using a 0.22 μm vacuum filter (Sigma). Here ...
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bioRxiv - Microbiology 2023Quote: ... approximately 8 L of seawater was filtered onto 0.22-μm Sterivex-GP pressure filter units (Millipore, Billerica, MA, USA). The filters were immediately soaked in 2 mL of RNAlater Stabilization Solution (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: We measured TEER in of RPE plated on transwell filter membranes using an Epithelial Volt- Ohm Meter (Millipore, #MERS00002) and ERS Probes (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were buffer exchanged into 1x PBS (pH8.0) using Amicon Ultra-15 Centrifugal Filter Unit (10 kDa) (Millipore) and flash-frozen.
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bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... THLE-2 cells were maintained on plates coated with 10% filter-sterilized collagen from calf skin (Sigma Aldrich, C8919) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess reagents were removed by MeOH/CHCl3 protein precipitation or concentration-dilution using a 0.5 mL Centrifugal Filter (3K, Millipore). Pellets were washed by 500 μL MeOH/H2O (9:1 ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein was concentrated to 0.6 mL using a 10 kD cut-off Amicon Ultra centrifugal filter (Millipore). The sample was then loaded into a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was further concentrated to volumes <1 mL using 100kDa MWCO Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). Tricorn 10/300 chromatography columns (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Microbiology 2022Quote: ... After full lysis the lysates were gently filtered through a 0.45 µm filter (either 33 mm Millex SLHV033RS Millipore, or 75 mm Nalgene rapid flow filters – Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 9 and 10 hrs post infection) 2 ml culture was filtered through a 0.45 µm filter (Millex, SLHV033RS, Millipore) and the filtrate was kept at 4°C until analysis (for a maximum of 2 weeks) ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing the TOC-TIC supercomplex were pooled and concentrated to 16 μl in a 100-kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Unit (UFC5100, Millipore).
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... in HEPES buffer was exchanged with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0) using YM30K centrifugal filter unit (Amicon ultra-0.5 mL, Regenerated cellulose, Merck Millipore Ltd.,). It was further diluted with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0 ...
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bioRxiv - Biochemistry 2022Quote: rAAV samples for native MS measurements were buffer exchanged into 75 mM ammonium acetate pH 7.5 by serial dilution using 50 kDa Amicon-0.5 MWCO centrifuge filter units (Sigma-Aldrich). For each sample ...
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bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
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bioRxiv - Bioengineering 2022Quote: ... Collected samples of conditioned medium were filtered (0.2 μm) and then concentrated ten-fold (Amicon Ultra centrifugal filters; 3 kDa cut off; Millipore).
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bioRxiv - Biochemistry 2022Quote: ... The mixture was concentrated to ~ 450 μM of protein using Amicon Ultra Centrifugal Filters unit 3KDa cutoff (Millipore Sigma). Crystals were grown at 4°C ...
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bioRxiv - Biophysics 2022Quote: Peak fractions of the PEAK3/14-3-3 complex were pooled and concentrated using an Amicon Ultra-0.5 30k MWCO centrifugal filter (Millipore). Immediately before preparing cryo-EM grids ...