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Citations for Millipore Sigma :
4401 - 4450 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The withdrawn volume was recorded in each operation and approximately 10 ml of culture medium from positive wells were filtered through disposable 0.22 μm polysulfone filters (Millipore Sigma). DNA was extracted using a modified phenol-chloroform method (Urakawa et al. ...
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bioRxiv - Biophysics 2020Quote: ... dialyzed against Milli-Q water and concentrated to 4 mg ml-1 using Amicon Ultra centrifugal filter units with Ultracel-50K (Merck Millipore). UMODe filaments were obtained by limited digestion of UMODfl with elastase (Jovine et al. ...
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bioRxiv - Biochemistry 2020Quote: The S-variant and RBD coupled bead sets were incubated with SARS-CoV-2 patient sera at 3 dilutions (1/100; 1/1000 and 1/10000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) at room temperature in the dark on a horizontal shaker ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions with high purity were identified by SDS-PAGE and concentrated using a centrifugation filter with a 10 kDa cutoff (Amicon, Millipore).
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bioRxiv - Bioengineering 2021Quote: ... 4 mL of MP suspension was placed in a centrifugal filter unit (MWCO 10,000, Amicon® Ultra, Millipore, MA, USA) and centrifuged at 8,000 rpm for 1 h to separate the un-entrapped PEITC ...
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bioRxiv - Bioengineering 2021Quote: ... Liposomal purification was accomplished with Amicon Ultra-4 centrifugal filter units with a 100kDa MWCO (Millipore Sigma, Burlington, MA, USA) to eliminate free 7,8-DHF in the mixture ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant was replaced with fresh trehalose-HEPES-ACSF and the resuspended cell pellet was strained with a 20 μm nylon net filter (NY2004700, Millipore). After resuspension in trehalose-HEPES-ACSF ...
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bioRxiv - Bioengineering 2020Quote: ... The solution was removed from the centrifuge and filtered through a syringe filter with a 25 mm diameter (Merck Millipore) into a clean syringe at least twice to ensure no precipitates were present ...
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bioRxiv - Bioengineering 2020Quote: ... The HTM cell culture supernatants were collected and concentrated using Amicon® Ultra Centrifugal Filters (Millipore Sigma, Burlington, MA, USA) for immunoblot analysis ...
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bioRxiv - Bioengineering 2020Quote: ... Dialyzed LNP solutions were concentrated using Amicon® Ultra centrifugal filter units with 10,000 Da molecular-weight-cut-off (Millipore). Hydrodynamic size and PDI of the LNPs were measured in dynamic light scattering using the Zetasizer Nano ZSP (Malvern Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing pure LPMO were identified by SDS-PAGE and subsequently pooled and concentrated using Amicon Ultra centrifugal filters (Millipore) with a molecular weight cut-off of 10 kDa ...
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bioRxiv - Microbiology 2020Quote: ... were sequentially filtered under a gentle vacuum through 10.0-μm and 0.2-μm pore-size polytetrafluoroethylene (PTFE) filters (Millipore, Burlington, MA). Sequential size filtering was implemented in order to separate vegetative acantharian cells and cysts from reproductive swarmer cells (< 5 μm ...
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bioRxiv - Biochemistry 2021Quote: ... mAbs were concentrated using Amicon Ultra-4 Centrifugal Filter Units with a 50 kDa cut-off (Millipore Sigma Cat # UFC805024) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... and the fractions containing the CCT single subunit were combined and concentrated by Amicon Ultra 100 kDa MWCO centrifugal filter (Millipore). The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The PLL-dye conjugates were purified by filtration through Amicon Ultra-0.5 mL centrifugal filters with a molecular weight cutoff (MWCO) of 3 kDa (Millipore Sigma). The final concentration of PLL was determined with a bicinchoninic acid (BCA ...
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bioRxiv - Biophysics 2021Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
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bioRxiv - Neuroscience 2020Quote: ... Cell-free supernatant was collected and concentrated 10-fold through Amicon-Ultra 100 kDa MWCO spin filter units (Millipore UFC905008) prior to aliquoting and storage at -80°C ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... the dried metabolite pellets were resuspended in 90 μl of the LC-MS-grade water (Milli-Q 7000 equipped with an LC-Pak and a Millipak filter, Millipore). Then ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated and buffer exchanged to the same buffer supplemented with 10% glycerol using a 10 kDa MWCO centrifugal filter (Amicon Ultra, Millipore). Aliquots were snap frozen in LN2 and stored at −80°C ...
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bioRxiv - Cell Biology 2021Quote: ... were pooled and buffer exchanged through iterative rounds of dilution and concentration on a 10 kDa MWCO centrifugal filter (Amicon Ultra, Millipore) into final storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... The virus band was extracted and buffer exchanged to the NTE buffer using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Millipore).
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bioRxiv - Biophysics 2020Quote: ... Proteins were eluted by adding 10 ml HND buffer supplemented with 0.1% ANAPOE-X-100 and 2.5 mM desthiobiotin and then concentrated using an Amicon Ultra 5,000 MW cutoff filter (Millipore, Billerica, MA). Protein purity was assessed by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Bioengineering 2020Quote: ... The collagen solutions were filtered with a 0.45 μm pore size syringe filter (Biomed Scientific) whereas fibrinogen solutions were filtered with a 0.22 μm pore size syringe filter (cat. no. SLGPM33RS, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... His-Trap fractions containing GFP-nanobody-6xHis were pooled and concentrated using a 10 kDa MWCO centrifugal filter (Amicon Ultra, Millipore). Concentrated GFP-nanobody-6xHis was applied to Superdex S75 Increase 10/300 GL Column connected to an AKTA FPLC (Cytvia ...
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bioRxiv - Cell Biology 2021Quote: ... The peak fractions containing Alexa-660-HaloTag-FHIP1B co-eluted with SNAP-FLAG were pooled and buffer exchanged through iterative rounds of dilution and concentration on a 100 kDa MWCO centrifugal filter (Amicon Ultra, Millipore) into final storage buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... were pooled and buffer exchanged through iterative rounds of dilution and concentration on a 100 kDa MWCO centrifugal filter (Amicon Ultra, Millipore) using GF150 buffer with 10% glycerol ...
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bioRxiv - Cell Biology 2021Quote: ... were pooled and buffer exchanged through iterative rounds of dilution and concentration on a 30 kDa MWCO centrifugal filter (Amicon Ultra, Millipore) using GF150 buffer with 10% glycerol ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing pure protein were pooled and buffer exchanged through iterative rounds of dilution and concentration on a 50 or 100 kDa MWCO centrifugal filter (Amicon Ultra, Millipore) into final storage buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned media of GFP- or G029442-expressing HEK293T cells was concentrated using 3kDa size exclusion filter tubes (Millipore, Burlington, MA) by a factor of 5-fold ...
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bioRxiv - Neuroscience 2020Quote: ... The diluted AAVs were then desalted and concentrated by centrifugation at 5000 rpm for 30 minutes at 20°C in a pre-rinsed Amicon Ultra-15 filter (Millipore) in 1X PBS-MK ...
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bioRxiv - Biochemistry 2021Quote: ... A cloudy liposome suspension was freeze-and-thawed 10x and extruded 17x through 100 nm polycarbonate filters (Merck Millipore, USA) to reduce multi-lamellarity and achieve a consistent size distribution of vesicles ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions of 1.5 ml were collected and those containing the target were concentrated with Amicon Ultra centrifugal filter units (50 kDa cutoff; Millipore) to a final concentration of around 8 mg/ml (20-25 μM) ...
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bioRxiv - Biochemistry 2021Quote: Conditioned cell culture medium was collected and concentrated 20-fold by using Amicon Ultra-0.5 mL 10K MWCO Centrifugal Filters (Millipore Sigma). LOX activity was measured with an Amplite Fluorimetric Lysyl Oxidase Assay kit (AAT Bioquest ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
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bioRxiv - Biochemistry 2021Quote: ... The pellet was dissolved in 100 μl of 20% methanol and a 1:10 dilution was filtered through a 0.20-μm polytetrafluoroethylene filter (Millipore, U.S.A).
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bioRxiv - Biochemistry 2020Quote: ... Mixture was incubated at RT for 18 hours and desalted into 150 mM ammonium acetate for MS analysis using 100 kDa MWCO spin filters (Millipore-Sigma).
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged Mistics and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to Tris buffer containing 40 mM imidazole in an Amicon centrifugal filter (Merck Millipore) with adequate cutoff ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture was centrifuged at 400 × g for 5 min and concentrated three times with a Centriprep centrifugal filter (Cat. 4310, Millipore) at 1,220 × g (20 min each ...
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bioRxiv - Microbiology 2020Quote: Selenomethionine incorporated SgEsaA235-829 was concentrated to 10mg/ml by spin filtration using an Amicon Ultra Centrifugal filter unit with a 30kDa pore size (Millipore). Concentrated protein was screened for crystallization with the MCSG Crystallization Suite (Anatrace) ...
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bioRxiv - Microbiology 2020Quote: ... The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: Fractions containing the DnaA-6xHis protein were pooled together and applied to Amicon Ultra Centrifugal Filters with 30 kDa cutoff (Millipore). Buffer was exchanged to Buffer A (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... A spore suspension (1 × 1011 spores/ml) produced from the maintained inoculum was filtered with a nylon filter with 20µm pore size (Merck Millipore Ltd.) to capture P ...
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bioRxiv - Plant Biology 2021Quote: ... After a 10 min centrifugation step (15,000 x g) the samples were filtered in a 96-well MultiScreen filter plate (0.65 μm, Millipore, Burlington, MA). Reverse-phase liquid chromatography was performed using a DionexUltimate 3000 Series LC system (HPG-3400 RS High-Pressure pump ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing pure DHFRHuman (>90% as judged by running samples on an SDS-PAGE gel) were pooled and using an ultra-centrifugal filter concentrated (Millipore) in storage buffer (50 mM KPO4 ...
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bioRxiv - Neuroscience 2020Quote: ... 140 µL of each sample supernatant was added to a separate Ultrafree-MC VV Centrifugal Filter (Sigma-Aldrich; LOT: R7JA29961) and centrifuged for 4 minutes at 8,000 g at original temperature ...
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bioRxiv - Biochemistry 2021Quote: ... samples were taken from the reactions and the soluble fractions were separated from the insoluble substrate particles using a 96-well filter plate (Millipore) operated with a vacuum manifold ...
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bioRxiv - Microbiology 2021Quote: ... The enriched CD138+ cells were incubated overnight in RPMI 1640 supplemented with 10% FBS in MultiScreen-HA Filter Plates (Millipore) pre-coated with SARS-CoV-2 S protein ...