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Citations for Millipore Sigma :
4451 - 4500 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The resulting suspension was transferred onto sterile 0,2 μm nitrocellulose filters (Advantec) that were placed over 10% TSB agar media (Sigma Aldrich) without antibiotic selection ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged GB1 and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to HEPES buffer containing 40 mM imidazole using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S75 Increase 10/300 column equilibrated in HEPES buffer without imidazole and a pH of 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... OD600 of yeast cultures were measured and supernatants collected by running the cultures through a Millipore filter using MultiScreen HTS Vacuum Manifold (Millipore) first ...
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bioRxiv - Microbiology 2021Quote: ... Samples taken from the reactor for analysis of acetate and ammonium were immediately centrifuged and filtered with a 0.22 µm pore size filter (PVDF membrane, Millipore, Ireland). PHB and biomass dry weight measurements are performed on 15 mL bioreactor samples ...
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bioRxiv - Cancer Biology 2020Quote: Solubility assays were performed using Millipore MultiScreen®HTS-PFC Filter Plates designed for solubility assays (EMD Millipore, Billerica, MA). The 96-well plates consist of two chambers separated by a filter ...
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bioRxiv - Cancer Biology 2021Quote: ... and EV-containing fractions were pooled and concentrated with a 10 kDa molecular weight cut-off Amicon Ultra-4 centrifugal filter unit (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: ... The reacted mixture was dialyzed in PBST buffer (DPBS with 0.05% v/v Tween 20) by a centrifugal filter with a 3 KDa molecular weight cut-off (Millipore UFC500324). The supernatant was immunoprecipitated by custom-made anti-RFP-beads (see Immunoprecipitation [IP] and Co-IP below) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was harvested after 48 and 72 h of culture and concentrated using an Amicon filter (100K NMWL, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... Fractions with high purity were identified by SDS– PAGE and concentrated using a centrifugation filter with a 10 kDa cutoff (Amicon, Millipore). MBP-FUS-CHOP fusion type II and MBP-CHOP proteins were then flash frozen in 25% glycerol.
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bioRxiv - Cell Biology 2021Quote: ... Filtered cells were pelleted at 300g for 5 minutes and the EV-containing supernatant was further filtered through using a 0.8um syringe filter (Millipore; SLAA033SB). The two-step double-enzymes digest method was performed as described 23 ...
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bioRxiv - Microbiology 2021Quote: ... and the dissolved high molecular weight DNA in the supernatant was concentrated to a volume of 100 μL using 50-kDa Amicon centrifugal filters (Millipore). The concentrated extract was cleaned of proteins and other non-genomic DNA organic matter using the PowerClean Pro DNA Cleanup Kit (MoBio) ...
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bioRxiv - Biophysics 2021Quote: ... The purified AVP–V2R–Gs complex was concentrated using a Amicon Ultra centrifugal filter (molecular weight cut-off of 100 kDa, Millipore) and then subjected to a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The main peak fractions were pooled and concentrated to ∼1 mg/ml using an Amicon Ultra 100K filter (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: ... The protein solution was then filtered through a 0.22 μm filter and free dye removed by buffer exchange into gel filtration buffer in Amicon ultra 10 kDa spin concentrator (Millipore). The extent of labeling was estimated based on absorbance at 280 and 647 nm.
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bioRxiv - Microbiology 2022Quote: ... The buffer was float on the Agar for 10 min and then it pumped out by syringe and filtered by PVDF sterile syringe filter (0.22 μm, Millipore, Ireland). To estimate the contribution of lysed host bacterium cells and diffused molecules from NA on the SF measurements ...
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bioRxiv - Biochemistry 2022Quote: ... The released glycans were collected by ultrafiltration using a filter with a molecular weight cut-off of 3,000 (Millipore, USA) at 12,000 rpm for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... was solubilized in 1 mL PBS to a stock concentration of 1mg/mL and sonicated with a probe tip sonicator for 5 seconds with 1-second pulse at 20% amplitude and then passed through a 1 mL syringe with a 0.22 μm filter (Millipore Sigma). Neurons were incubated with 25 μM of DQ-BSA Red solution diluted into neuronal media for 2 hours at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... with 150 mM KCl and 0.5 mM EDTA at 4C using Amicon Ultra-4 centrifugal filter unit (molecular weight cut-off of 10,000 Da) (Millipore Sigma). The Fab yield was quantified by measuring absorbance at 280 nm on a Nanodrop One (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and filtered through a 0.45μm membrane via centrifugation (Durapore PVDF centrifugal filters, Millipore, Billerica, MA, USA). The filtrate was kept on ice until analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample was filtered through a 0.22 μm centrifugal filter and loaded onto a HiLoad 16/60 Superdex 200 prep grade size exclusion column (Millipore Sigma) equilibrated in 25 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The purified RNA was buffer exchanged three times into RNase-free water using 30 kDa cutoff Amicon centrifugal filters (Millipore). The RNA was stored at -20°C until preparation of cryo-EM grids.
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bioRxiv - Biochemistry 2022Quote: ... Immobilized pepsin was removed by centrifugation at 1,000 x g for 30 sec at 0 °C using a 0.65 µm filter inlet (Merck Millipore, Darmstadt, Germany) and the flow through was injected immediately into the HPLC-MS/MS system ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing MbxA were pooled and concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore). Histidine was removed with a PD10 desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was purified by collecting the flow-through from a second Ni-Sepharose column and concentrated using Amicon spin filters (Millipore). Protein was stored at -20 □.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cell media was filtered with an Amicon Ultra-0.5 Centrifugal Filter Unit (MWCO 10 kDa, Millipore Sigma, Burlington, MA, USA). 5-MeO-DMT was quantified using a UPLC – Xevo TQ-S micro MS system (Waters Corporation ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions containing pure Pae87 were desalted and concentrated with Amicon Ultra centrifugal filters of 3 kDa or 10 kDa MWCO (Millipore). Concentrated samples were centrifuged at 15,000 × g for 10 min prior to crystallization trials ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Immunology 2022Quote: Immune complexes comprising inactivated CV-A21 viral particles and CV-A21-elicited polyclonal antibodies were concentrated to 2.5 mg/ml using Amicon Ultra centrifugal filter units with 100 kDa cutoff (Millipore Sigma). Cryo-grids were prepared on a Vitrobot mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Fractions corresponding to Coxsackievirus A21 particles were pooled and concentrated to 0.5mg/ml using Amicon Ultra centrifugal filter units with 100 kDa cutoff (Millipore Sigma).
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bioRxiv - Microbiology 2022Quote: ... Medium was harvested 48 and 72 hours post transfection and filtered through a 0.45 µM filter (Merck Millipore, Overijse, Belgium). MLV was concentrated by ultrafiltration using Vivaspin MWCP 50 KDa filter tubes (Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: ... the PSI-tetramer fraction was obtained in a 25–30% trehalose layer and then concentrated using a 100 kDa cut-off filter (Amicon Ultra; Millipore) at 4,000 × g.
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bioRxiv - Microbiology 2022Quote: ... and buffer exchanged three times into protease buffer (20 mM Tris pH 8.0, 10% glycerol) using an Amicon Ultra centrifugal filter (Sigma-Aldrich). Purified His6-tagged TEV protease was added to all proteins at a 1:20 ratio (except for SpoVD ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: All of the water samples were initially filtered by a vacuum filter with 0.2-μm pore-size polycarbonate membranes (Millipore, USA). The filters were used to extract iDNA by FastDNA™ Spin Kit for Soil (MP Biomedicals ...
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bioRxiv - Neuroscience 2022Quote: ... Viral particles were collected at 72 hours by filtering spent medium through a 0.45 μm cellulose acetate filter (Millipore Sigma), then concentrating the supernatant with AAVpro® concentrator (TaKaRa).
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was collected from the column and concentrated approximately 10-fold using an Amicon Ultra Centrifugal Filter Unit (Millipore) with a 3,000 Da cutoff ...
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bioRxiv - Cancer Biology 2022Quote: ... The media from both uninfected and infected cells was retrieved from the flasks and filtered through a 0.22 μm syringe filter (Millipore Sigma). For proliferation assays ...
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bioRxiv - Cancer Biology 2022Quote: ... Pooled samples were diluted to a total volume of 500 μL and transferred to Amicon Ultra-0.5 30 kDa centrifugal filter devices (Millipore Sigma). Filter devices were centrifuged at 14000 g for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial culture from each sample was concentrated using cellulose syringe filters with a 0.22 μm pore size (Millipore, Burlington, MA). P ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Immunology 2022Quote: ... with a SuperdexTM 200 Increase 10/300 GL (Cytiva) prior to up-concentration using Amicon® Ultra-0.5 Centrifugal Filter Units (Merck Millipore). The protein eluted as a dimer.
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bioRxiv - Microbiology 2022Quote: ... the collected supernatants of each isolate were concentrated using a Centricon Plus-70 centrifugal filter device (100 K nominal molecular weight limit; Millipore) resulting in an approximately 100x concentrated stock (v/v ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated to 30-40 mg ml-1 using a 50-kDa cutoff Amicon Ultra centrifugal filter unit (Millipore). NpSxph crystals were obtained by hanging drop vapor diffusion at 4°C using 1:1 v/v ratio of protein and precipitant ...
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bioRxiv - Biophysics 2022Quote: ... Betaine uptake was measured at various intervals after cell samples were passed through glass fiber filters (APFF02500, Millipore, Schwalbach, Germany), and washed twice with 2.5 ml of 0.6 M KPi buffer ...
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bioRxiv - Biophysics 2022Quote: ... filtered through a 0.45 μm PES filter and concentrated at 4C using an Amicon Ultra-15 centrifugal filter unit (molecular weight cut-off of 10,000 Da) (Millipore Sigma). Protein was further purified using CaptureSelect CH1-XL affinity resin (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... The purified protein was desalted and exchanged into 50 mM HEPES buffer containing 50 mM NaCl (pH 7.2) with an Amicon Ultra-15 centrifugal filter (10 kDa MWCO, Merk Millipore Inc.). Protein was diluted to the required concentration before in vitro assays.
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bioRxiv - Molecular Biology 2022Quote: ... The incubated RdRp complex was then concentrated with a 100 kDa molecular weight cut-off centrifugal filter unit (Millipore Corporation) and then further purified by size exclusion chromatography using a Superdex 200 10/300 GL column in 25 mM TrisHCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... solution at pH 7.4 and concentrated to 0.5 mg/ml using Amicon Ultra centrifugal filters with a 10 kDa molecular weight cutoff (Millipore, UFC901096). Dithiothreitol (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing AniA were concentrated over an Amicon® Ultra-15 Centrifugal Filter Unit with 30 kDa MWCO (Millipore). The buffer was exchanged to 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... glutathione or flag peptide were removed by buffer exchange and the purified proteins were concentrated on Amicon Ultra 50K filters (Millipore). Purified proteins were recovered in 20 mM Hepes ...