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Citations for Millipore Sigma :
4501 - 4550 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Fractions containing full-length CLAMP were identified by SDS-PAGE and concentrated using a centrifugation filter with a 10 kDa cutoff (Amicon, Millipore) and frozen as aliquots.
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were diluted to a final concentration of 50 mM NaCl and then concentrated on Amicon centrifugal filters 30K for FHL and 10K for LIM domains (Millipore). The proteins’ purities and identities were confirmed by SDS-PAGE and mass spectrometry ...
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Microbiology 2020Quote: ... the sorted sup-population were gently filtered on 13 mm diameter polycarbonate filters (GTTP, 0.2 μM pore size, Millipore, MA), washed twice with sterile sea water and air-dried ...
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was removed and filtered through a 0.45 µM filter and then concentrated using an Amicon concentrator (UFC910024 from Sigma Millipore).
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). For infectious SARS-CoV-2 immunization ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...
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bioRxiv - Developmental Biology 2021Quote: ... Carcasses were removed and the cell solution was transferred to a 1.5 mL Eppendorf tube containing 800 μL fresh media and then passed through at 70 μm Flowmi tip filter (Sigma). Cells were analysed on a MACSQuant Analyser.
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bioRxiv - Developmental Biology 2021Quote: ... The filtered supernatants were concentrated using a 100 kDa MW centrifugal filter (Amicon Ultra-15; EMD Millipore, Billerica, MA, USA) at 5,000 × g and then ultracentrifuged in a Beckmann optima XE with a SW 41 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... each sample was filtered through a net (size 27 μm) and then filtered through nitrocellulose filter with a pore size of 0.22 μm (Millipore, France), and the material from the filter was transferred to sterile flasks with 20 mL of lysis buffer (40 mmol L-1 EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
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bioRxiv - Microbiology 2020Quote: ... Up to 6.9 L of seawater from each sampling depth were filtered through a series of 20 μm Nylon Net filters (Millipore), 5 μm polycarbonate membrane filters (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Fractions that strongly reacted with both antibody pairs were pooled and concentrated using a 10 kDa Ultra-15 centrifugal filter (Millipore-Sigma).
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bioRxiv - Microbiology 2021Quote: ... The two aqueous phases were combined and then concentrated to a final volume of 2 mL by ultra-filtration using a 10 kDa MWCO Ultra-15 centrifugal filter (Millipore-Sigma).
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bioRxiv - Biophysics 2020Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
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bioRxiv - Immunology 2021Quote: ... After digestion for 2 min in a water ice bath the samples were separated by centrifugation at 1000 x g for 30 s at 0°C using a 22 µm filter (Merck, Millipore) and were immediately analyzed by LC-MS ...
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bioRxiv - Neuroscience 2020Quote: ... FVB/NJ primary microglia harvested from mixed glia cultures by the shaking method were seed onto Millicell cell culture inserts with 8-μm pore filter (Millipore), and treated with 50 μM GM1 for 4h or with 10 μM L-t-PDMP or 5 μM GENZ-123346 for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... After digestion the sample volume was brought to 100 μl with ddH20 followed by filtration using 0.22 μm Millex Syringe Filters (EMD Millipore). 5 μl of the filtered solution was analyzed by LC-MS/MS ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... The digested samples were spun at 3,364xg for 5 minutes and samples were transferred to a cellulose acetate filter plate (Millipore, MSUN03010) and spun at 3,364xg for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... clarified by centrifugation at 2,500 rpm for 10 mins and purified through a 0.45um pore-size filter (Millipore, Billerica, MA). Transfection derived supernatants were subsequently briefly passaged in either human PBMC while all other primary isolates were briefly passaged in PBMCs to generate virus stocks ...
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bioRxiv - Neuroscience 2021Quote: ... 200µl of total protein was further fractionated into soluble and aggregated forms using membrane based centrifugal filter unit (Millipore MRCF0R100) 500X g for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing EfgA were pooled and concentrated in an Amicon centrifugal filter concentrator with a 10 kDa cutoff membrane (Millipore). The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Distilled water was soaked off by a filter paper and the grid was stained with 5 μl 1% uranyl acetate (Sigma) in water for 7 sec ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Immunology 2020Quote: Supernatants from epithelial cells were centrifuged at 5000g for 1 hr at 4 °C and the residual volume collected using 3 kDa molecular weight cut off 50 mL or 15 mL tube filters (Millipore). The lower volume was collected and volume measured then the residual volume from the upper level was diluted to a similar volume to the lower fraction ...
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bioRxiv - Neuroscience 2021Quote: ... individual SEC peak fractions were pooled and pre-concentrated with an Amicon Ultra 4mL centrifugal filter (Merck Millipore, 30kDa NMWCO). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and fractions containing protein of the appropriate size were pooled and concentrated using 10 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Millipore, UFC505024) into buffer without imidazole (20 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The only difference between the base medium and drug-containing medium was the addition of filter-sterilized Ciprofloxacin (Sigma-Aldrich). Starting populations of E ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions were desalted into 1× BRB80 and 10 μM GTP with a PD10 desalting column and concentrated using an Amicon Ultra 30K MWCO centrifugal filter (Millipore). Tubulin was aliquoted and snap frozen in liquid nitrogen.
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bioRxiv - Biochemistry 2020Quote: ... culture supernatant was centrifuged at 400 g for 5 min and the supernatant was filtered by 0.22 μm PES syringe filter (Millipore, SLGP033RB). The filtered supernatant was concentrated by Amicon Ultra 2 mL 10K (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... Rsa4-GFP or Ytm1-GFP were diluted to 100 nM and filtered using a 0.22 μm centrifugal filter (Millipore UFC30GV00), and mixed 1:1 with MIDAS protein (initial stock 80 μM ...
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bioRxiv - Biophysics 2021Quote: ... The fractions of the flow-through containing MSP protein were pooled together and concentrated with a 10 kDa Amicon Ultra-filter (Millipore) to 100-200 μM ...
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bioRxiv - Biophysics 2020Quote: ... Unbound dye was removed by repeated dilution and centrifugation through an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore Sigma), prior to size exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... cells were removed from the filtrate by centrifugation (3000 x g for 15 min) and filter sterilization (Millipore GP express Steritop, 0.22-µm pore size (EMD Millipore) or 25-mm Pall Acrodisc with 0.22-µm pore size Supor membrane (Pall Corporation) ...
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bioRxiv - Microbiology 2021Quote: ... All samples were further concentrated to a final volume of 1-2 ml using 100 kDa Amicon-ultra filters (Merck Millipore).
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bioRxiv - Bioengineering 2020Quote: ... and the supernatants were harvested 48 hr and 72 hr post-transfection before passing through a 0.22-µm filter (Millipore, USA) and ultracentrifugation at RPM 25,000 at 4°C for 2 hr ...
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bioRxiv - Bioengineering 2020Quote: ... The EV sample was collected according to the 280 nm UV absorbance chromatogram and concentrated using an Amicon Ultra-15 10 kDa molecular weight cut-off spin-filter (Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... up to 100 μg of carrier-free IgG antibody was subjected to buffer exchange by washing with R-buffer using a 50 kDa Amicon filter (Millipore) that was pre-soaked with R-buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The assembled pores were then purified to remove excessive DNA strands using a 100k MWCO Amicon Ultra centrifugal filter device (Millipore). Following purification ...
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bioRxiv - Molecular Biology 2022Quote: ... After it was fully thawed the buffer was mixed by pipetting and then filtered with a 0.8 um syringe filter (Millex-AA, SLAA033DD, Millipore Sigma) to remove any cells or large cellular debris ...
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bioRxiv - Microbiology 2022Quote: ... uninfected and gonococci-infected Pex cell culture supernatants were collected and concentrated 10-fold using Centricon YM-30 (molecular weight cut off (MWCO) greater than 30 kDa) centrifugal filter units (Millipore). The filter retentates were collected and used to line 96-well microtiter plates ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dialyzed samples were further concentrated to 10 mg mL-1 using Amicon® Ultra-15 Centrifugal Filter Unit (Merck Millipore). The final concentration of glycerol was adjusted to 50% (v/v% ...
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bioRxiv - Neuroscience 2022Quote: ... The suspension containing the SGNs was filtered through a 40-μm cell filter and planted on glass coverslips pretreated with 0.5 mg/ml poly D-lysine (Sigma-Aldrich) and 1 mg/ml laminin (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Pelleted DNA was resuspended in 1 ml of TLE and washed twice by dyalisis with 8 ml of TLE using AMICON Ultra Centrifuge Filter Unit 15mL 30K (Millipore UFC903024 ...
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bioRxiv - Immunology 2022Quote: ... tachyzoites were purified from HFF cultures by needle-passaging scraped cells and filtering the parasites through a 5.0-μm filter (EMD Millipore). Mice were then inoculated i.p ...
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bioRxiv - Neuroscience 2022Quote: ... additionally filtered with a 735 nm LP filter eliciting an isomerization rate of ~17 R*/s) in oxygenated Ames’ medium (Sigma) with constant bubbling (95% O2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... normal (24 h) and low pH (24 h) cultures were filtered using a 0.22 μm PVDF filter (Merck Millipore, Germany). These samples were loaded onto an OnGuard column (Dionex OnGuard II Cartridge ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was concentrated to 30mg/ml with Amicon centrifugal filter units with 100 kDa molecular weight cutoff (Sigma Millipore).