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Citations for Millipore Sigma :
4051 - 4100 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The fractions containing the TOC-TIC supercomplex were pooled and concentrated to 16 μl in a 100-kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Unit (UFC5100, Millipore).
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... in HEPES buffer was exchanged with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0) using YM30K centrifugal filter unit (Amicon ultra-0.5 mL, Regenerated cellulose, Merck Millipore Ltd.,). It was further diluted with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0 ...
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bioRxiv - Biochemistry 2022Quote: rAAV samples for native MS measurements were buffer exchanged into 75 mM ammonium acetate pH 7.5 by serial dilution using 50 kDa Amicon-0.5 MWCO centrifuge filter units (Sigma-Aldrich). For each sample ...
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bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
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bioRxiv - Bioengineering 2022Quote: ... Collected samples of conditioned medium were filtered (0.2 μm) and then concentrated ten-fold (Amicon Ultra centrifugal filters; 3 kDa cut off; Millipore).
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bioRxiv - Biochemistry 2022Quote: ... The mixture was concentrated to ~ 450 μM of protein using Amicon Ultra Centrifugal Filters unit 3KDa cutoff (Millipore Sigma). Crystals were grown at 4°C ...
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bioRxiv - Biophysics 2022Quote: Peak fractions of the PEAK3/14-3-3 complex were pooled and concentrated using an Amicon Ultra-0.5 30k MWCO centrifugal filter (Millipore). Immediately before preparing cryo-EM grids ...
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bioRxiv - Biophysics 2022Quote: ... CM was concentrated ≈300 times to 0.5 mL using a Centricon Plus-70 centrifugal filter device with a 10K nominal molecular weight limit (Merck Millipore).
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bioRxiv - Bioengineering 2022Quote: ... The clarified supernatant was then filtered with a 0.45 um cellulose acetate filter (Sigma-Aldrich, cat. code CLS430516-12EA), poured into a sterile 750mL bottle along with 6% w/v PEG-8000 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was harvested after 24 and 48 h of incubation and filtered through a 0.22 μm syringe filter (Millipore). To infect primary myoblasts ...
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bioRxiv - Cell Biology 2022Quote: ... passed through a 0.45 µm nylon filter and used to transduce PSCs in the presence of 8 μg/mL polybrene (Sigma) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were prepared by filtering cells though 70μm filters after which cells were incubated in Red Blood Cell Lysis buffer (Sigma) for 3min at 37C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Fab protein was eluted with 50 mM acetate buffer at pH 5.0 and concentrated using Ultra-15 Centrifugal Filters (Millipore). Protein concentration was determined using the Qubit protein assay (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... Samples were centrifuged at 13,000 rpm for 10 min and the SUP was filtered through 0.2 μm nylon filters (Sigma CLS8169) before use.
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bioRxiv - Microbiology 2022Quote: Cells were then pelleted at 7000 × g for 3 min and resulting supernatants filtered through a 0.2 μm PVDF filter (Millipore). The no bacteria “fresh” 5-FU controls were also filtered in this way to account for any potential loss of 5-FU by filtration ...
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bioRxiv - Microbiology 2022Quote: ... The purified NS1 stocks were then subjected to dialysis against 1X PBS for 48 hours at 4°C and concentrated using Amicon filters with 10,000 molecular weight cut-off (Millipore). The Pierce BCA protein quantitation kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... They were then pooled and concentrated ten-fold using the Amicon Ultra centrifugal filter units (Sigma-Aldrich, Catalog # Z648043) with a MWCO of 100 kD.
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bioRxiv - Microbiology 2022Quote: ... The purified and concentrated solution of 60% sucrose density gradient pellet was then cleaned using an Amicon Ultra-0.5 (10 kD) centrifugal filter (Millipore) for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2022Quote: ... After concentrating the protein using a 30 kDa molecular weight cut-off Amicon Ultra-15 centrifugal filter unit (Millipore) the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
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bioRxiv - Plant Biology 2022Quote: ... The eluates were desalted via centrifugation with a salt-free buffer (50 mM HEPES-NaOH (pH 7.5)) using Amicon Ultra centrifugal filters (30K MWCO, Millipore). The centrifugation step was repeated five times ...
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bioRxiv - Microbiology 2022Quote: ... The eluted solution was concentrated and desalted with preservative buffer (PBS) in an Amicon Ultra centrifugal filter devices with a 50,000 molecular weight cutoff (Millipore). The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The eluate was then dialyzed overnight in PBS and protein was concentrated by using Amicon Pro centrifugal filter (Millipore). The protein was quantified by using BCA Protein Assay Kit (Thermofisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and 15 mL of supernatants were transferred into a 30 kDa Amicon® Ultra-15 Centrifugal Filter Units (Sigma). The residue from the filtration device was then transferred to a 1.7 mL microfuge tube and spun at 12,000xg for 5 minutes to pellet any remaining cells ...
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bioRxiv - Microbiology 2022Quote: SEC-purified LCMV pfGP-TD and LCMV pfGP-TD/M28 Fab complex in TBS was concentrated to 0.8 and 1.2mg/mL using a 30K Amicon centrifugal filter (Sigma Aldrich). For LCMV pfGP-TDapo ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatant was passed through a 1.2 µM filter before batch binding to ANTI-FLAG M2 affinity resin (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated for a further 48 hours before all media was collected and filtered using a 0.45 µm filter (Sigma). Equally loaded tubes (Beckman ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were filtrated through 0,45 µm pore-sized cellulose membrane filters (Millipore Sigma Aldrich, St. Louis, MO) with a vacuum/pressure pump system (Pall Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Sequential fraction 3.5 until 5.5 mL was collected from the flow-through and further concentrated using the Amicon Ultra 0.5 mL 10 kDa filter (Millipore). To isolate EV-associated lipids ...
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bioRxiv - Neuroscience 2022Quote: ... fractions corresponding to Atx3 were pooled and concentrated on an Amicon Ultra-15 centrifugal filter (10 kDa MWCO, Millipore) to 20-30 mg.mL-1 in buffer A (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... After 24 and 48 h the supernatants containing viral particles were collected and filtered using a 0.22 µm syringe filter (Millipore).
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 0.5 mL centrifugal filter units (3-kDa molecular weight cutoff, Millipore), using centrifugation at 14,000 g ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were concentrated by 30 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore, Cat. No. UFC903096).
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bioRxiv - Molecular Biology 2023Quote: ... Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Synthetic Biology 2022Quote: ... The folded constructs were purified from staple strands excess using the Amicon Ultra 0.5 mL 100 kDa centrifugal filters (Millipore). Capped Amicon Ultra were rinsed with 500 μL of RNase free folding buffer and centrifuged at 14000g for 1 min at 11 °C ...
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bioRxiv - Immunology 2022Quote: ... Fractions containing IgG were identified by SDS-PAGE under reducing/non-reducing conditions and concentrated using Centrifugal Filters (Millipore). Protein concentration was quantified with the BCA Assay Kit (Beyotime ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then filtered our playa suspensions into glass funnels through autoclaved 5.0 μm filters (Millipore-Sigma, Burlington, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then filtered our playa suspensions into glass funnels through autoclaved 5.0 μm filters (Millipore-Sigma, Burlington, MA, USA).
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bioRxiv - Microbiology 2022Quote: Cleaved protein was concentrated to ~1 mL using an Amicon Ultra-15 centrifugal filter 30 kDa MWCO (EMD Millipore), then loaded onto a HiLoad Superdex S200 pg gel filtration column (GE Healthcare Life Science) ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions from the single monomer peak were combined and diluted ten-fold and buffer exchanged three times into 10 mM sodium phosphate buffer at pH 6.5 with an Amicon Ultra-15 Centrifugal Filter Unit Ultracel-10 (Millipore) by centrifugation at 3,000g at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The ‘Positive Cell Detection’ function was used to identify Ki67+ and Ki67-cells using the following parameters: Nucleus Parameters (Requested pixel size 0.5 µm, Background radius 8 µm, Median filter radius 0 µm, Sigma 1.5 µm ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were collected at 42-48 h after transfection and filtered through a 0.45 um low-protein binding filter (Millipore). Patient-derived TCL were infected with lentiviral supernatants containing polybrene (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were prepared in PBT and were filtered through a syringe filter (Millipore Millex-GV 13mm x 0.22um). The secondary antibody solution was added to the slides and incubated for 60 minutes at room temperature in a humidified chamber ...
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bioRxiv - Microbiology 2024Quote: ... from the resin was immediately neutralized with 1/10 faction volume of 1 M Tris-HCl pH 8.5 and concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 100kDa NWMCO (Merck Millipore), in which the elution buffer was exchanged with PBS (GIBCO).
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bioRxiv - Neuroscience 2023Quote: ... Sections were protected from an excessive pressure wave by interposing a filter between the section and the gene gun (3μm pore size, 8x105 pores/cm2; Millipore). The filter also prevented large clusters of dyes from landing on the sections ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...