Labshake search
Citations for Millipore Sigma :
4201 - 4250 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The collected peak fractions were concentrated to ~0.8 mg/ml using Amicon Ultra centrifugal filter (100-kDa cut-off; Millipore) and immediately used for the cryo-EM grid preparation for the hTMEM87A structure.
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of supernatant was concentrated and desalted using an Amicon Ultra-0.5 centrifugal filter device according to the manufacturer’s instructions (Millipore, Inc.), re-constituted in an equal volume of 50 mM Tris 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Transfected cells were grown for 72 hr and then the supernatant containing the viral particles was collected and passed through a sterile 0.45 µm Millex syringe filter (Millipore). HEK293 FLAG-Cas9 cells (a gift from Alice Ting ...
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bioRxiv - Cancer Biology 2022Quote: ... centrifuged for 10 min at 200 x g and filtered thought a 0.45 μM filter (Millipore, Temecula, CA, USA) and concentrated using Vivaspin concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell debris was removed by spin at 400 RCF for 5 min followed by filtration through 0.45-μm PVDF syringe filter units (Millipore).
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bioRxiv - Immunology 2023Quote: ... For lab scale: supernatant was subjected to concentrating filtration against a Centricon Plus-70 Centrifugal filter unit (Millipore, UFC710008), then subjected to size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2023Quote: ... For lab scale: supernatant was subjected to concentrating filtration against a Centricon Plus-70 Centrifugal filter unit (Millipore, UFC710008), then subjected to size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Biochemistry 2023Quote: ... MWRA flow-through protein solution was concentrated to ∼15 mL using a 30 kDa MWCO centrifugal filter (EMD Millipore), ensuring not to concentrate to where solution became yellow/cloudy and viscous ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 30 K (Merk Millipore). Finally ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
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bioRxiv - Biochemistry 2023Quote: ... 5 and 7 hours 100 µL of were transferred from each reaction to a 96-well MultiScreenHTS HV Filter Plate (0.45 µm pore size; Millipore), and the reactions were stopped by vacuum filtering using a Vac-Man 96 Vaccum Manifold (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell culture media was changed the following day and lentiviral supernatant was harvested 48 h and 72 h later and filtered through a 0.45 μm filter (Millipore). Lentiviruses were aliquoted and stored at - 80 °C until use.
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected and concentrated 10-fold in an Amicon centrifugal filter unit (MWCO 3k Da, EMD Millipore) and subsequently diluted 5-fold with MSC media ...
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bioRxiv - Bioengineering 2023Quote: ... The eluted fraction was concentrated 10 times using 100 kDa Amicon Ultra-15 centrifugal filters (Millipore Sigma, Cat# UFC903008) and buffer exchanged onto a pre-equilibrated PD-10 column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 50 mM Tris-HCl (pH 7.5) buffer and concentrated the eluate with a pre-washed 10 kDa MWCO Amicon Ultra spin filter (Millipore). The concentrated eluate was buffer-exchanged into 20 mM K-phosphate (pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Viruses were concentrated from the supernatant after 48 hours using Amicon® Ultra-15 Centrifugal Filters (Millipore UFC-9100). MCF-10A cells were transduced with the concentrated viruses ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants (30ml) were clarified by centrifugation (5 min at 800g) and filtered through a 0.2μm diameter pore filter (Millipore, MA) to remove cell debris and the viral biofilms potentially detached from cultured cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell supernatant containing lentivirus was collected at 48-72 hr after transfection and filtered with 0.45□m filter (Millipore). For infection into MDA-MB231 cells ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was carefully collected without disturbing the loose pellet and sterile-filtered through a 0.22 µm PVDF filter bottle (Millipore), aliquoted and frozen at -20°C until used.
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bioRxiv - Microbiology 2023Quote: ... cells were grown to an OD600 of 0.3 in 4 mL and filtered through 0.22 μm nylon filters (Millipore GNWP04700). The cells were quenched by placing the filter upside down in a 60 mm dish containing 1.2 mL pre-chilled quenching solution (40:40:20 Acetonitrile:methanol:H2O + 0.5% formic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned medium containing recombinant lentivirus was collected 48hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 μm (Merck Millipore). Samples of these supernatants were applied immediately to target cells together with Polybrene (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... diluted the reaction mixture to 50 μL with water and filtered the samples through 0.22 μm Millex-GV PVDF filters (Millipore). Five microliter sample was injected into ZORBAX SB-Aq 4.6 x 50 mm column (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Plant Biology 2023Quote: ... production we centrifuged YM-grown bacteria at 8,220 × g for 20 min and filtered the supernatant through 0.22 μm cellulose filters (Millipore, USA). Then ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated and buffer exchanged into 1x PBS or 1x TBS (20 mM Tris, 150 mM NaCl, pH 8.0) using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa) (Millipore). Purified proteins were snap frozen and stored at – 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg of soluble proteins in TEAB buffer were added onto 30 kDa cutoff filter (Sigma-Aldrich, cat#MRCF0R030) and centrifuged at 11,000 g for 15 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using centrifugal filter units (Amicon, 10KDa cut-off; Millipore, Ultra-4 UFC801024 and/or Ultra-0.5 UFC501024) ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated and buffer exchanged into 1x TBS (20 mM Tris, 150 mM NaCl, pH 8.0) using Amicon Ultra-15 Centrifugal Filter Unit (100 kDa) (Millipore). Purified proteins were snap frozen and stored at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... the antibody-linker solution was washed once using a 30 kDa Amicon Ultra-2 centrifugal filter (UFC203024, Sigma-Aldrich). Annealed DNA oligonucleotides with a 5′ azide modification (Integrated DNA Technologies ...
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bioRxiv - Immunology 2023Quote: ... The eluted solution was desalted and concentrated with preservative buffer (PBS) in an Amicon Ultra centrifugal filter device with a 50,000 molecular weight cutoff (Millipore). The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Microbiology 2023Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3 kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Developmental Biology 2023Quote: ... filtered through 0.45 μm sterilization filters and added to HC11 cells together with 8 μg/mL polybrene (Sigma-Aldrich). Transduction efficiency was monitored by checking the expression of green fluorescent protein (GFP) ...
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bioRxiv - Microbiology 2023Quote: ... were filtered using vacuum filtration (ca. -200 mm Hg) onto 47 mm diameter 0.2 µm hydrophilic Durapore filters (Millipore). Samples were immediately flash-frozen and stored in liquid nitrogen (-196 °C ...
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bioRxiv - Microbiology 2023Quote: ... Fractions corresponding to CVB5 were combined and concentrated to 4.5 mg ml-1 using Amicon Ultra centrifugal filter units with 100 kDa MWCO (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50 kDa cut-off (Millipore-Sigma) and quantified using a NanoDrop spectrophotometer (280 nm) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 4% SDS and 0.1 M DTE) and buffer exchange was performed in Amicon Ultra Centrifugal filter devices (0.5 mL, 30 kDa MWCO; Merck Millipore) at 14,000 rcf for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered with a Spin-X centrifuge tube filter (Costar) with a pore size of 0.22 µM and concentrated using a centrifugal spin concentrator (Millipore) with an MWCO of 10 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphopeptides were eluted in 1% ammonia and clarified by filtration using a 0.45 μm filter plate (Millipore, Sigma–Aldrich) before being loaded onto Evotip Pure trap columns.
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bioRxiv - Cell Biology 2023Quote: ... Conditioned media were collected after 48 h and centrifuged at 3,000 g for 10 minutes before passing through a 0.22 μm Millex® syringe filters (Merck Millipore). For viral infection ...
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bioRxiv - Cell Biology 2023Quote: ... Eluates were pooled together and concentrated to ∼75 μL using a centrifugal filter (Amicon Ultra 0.5 mL 3,000, Millipore UFC500324), supplemented with 25 µL of NuPage 4x LDS sample buffer (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell debris were removed by centrifugation at 30,000×g and 4 °C for 60 min and the lysate was filtered through 45 μm filters (Millipore). The lysate was loaded onto a Fractogel DEAE anion exchange column (Merck) ...
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bioRxiv - Biophysics 2023Quote: ... The media was centrifuged for 5 minutes at 500 g and filtered through a 0.45 μm syringe filter (Millipore). The media was concentrated with a UV-sterilized Amicon ultra-15 100 kDa centrifugal filter until the solution was concentrated by 30 times its original volume ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to 0.5 mL with a Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). Finally ...