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Citations for Millipore Sigma :
3951 - 4000 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA, Sigma, cat. no A4544), 3 µM CHIR-99021 (Axon Medchem ...
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bioRxiv - Microbiology 2023Quote: The deep-well plate with the drugs and communities was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated anaerobically at 37 °C for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... NIH/3T3 cells were seeded at a density of 200 cells/well on a 96-well plate coated with fibronectin (Sigma, F4759). After seeding ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the filtered peptides eluted in 0.1% TFA in 50:50 acetonitrile and UHQ water are spotted in triplicate on a MALDI plate along with α-cyano-4-hydroxycinnamic acid (CHCA, Sigma) as a matrix solution ...
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bioRxiv - Developmental Biology 2024Quote: ... from the previously characterized Rex1-GFP line52 were cultured on tissue culture plastic Nunc plates that had been coated with 10µg/ml Laminin-111 (Sigma-Aldrich L2020) in PBS ...
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bioRxiv - Genetics 2024Quote: ... The samples were then transferred to an enzymatic digestion buffer (200 µl, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... the plates were analyzed using a GloMax® Explorer Multimode Microplate Reader after incubating with Resazurin (16 µg/ml; Sigma-Aldrich) for 6–8 h.
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bioRxiv - Synthetic Biology 2024Quote: ... Successfully recombinant clones switched the mCherry gene for EPO and were single-cell sorted into flat-bottom Corning 384-well plate (Sigma-Aldrich) containing 30 µL of CD CHO medium with 8 mM glutamine and 1.5% HEPES buffer (Gibco™ ...
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bioRxiv - Cancer Biology 2024Quote: Triplicates of WT and SRSF2P95H/+ K562 cells were seeded at 10,000 cells per well in 96-well plates and treated with indicated concentrations of CHIR (Cayman #13122) or Chloroquine (Sigma #C6628). Four days after culture ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells migrated for 2h and were then stained with calcein-AM and pipetted into a 96-well conical bottom plate (Sigma-Aldrich). A plate reader was used to measure florescence from the top of the plate at an excitation wavelength of 485 nm and an emission wavelength of 520 nm ...
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bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... 50µl from each well were transferred to a glass-bottom 384-well microscopy plate (Matrical Bioscience) coated with concanavalin A (Sigma-Aldrich). The logarithmic phase cultures were allowed to bind to the bottom of the plate for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... HepG2 cells were seeded in 96-well plates at a density of 5,000 cells per well in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich, Shanghai, China) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Neuroscience 2024Quote: ... CGNs were seeded in 12-well plates with cover glass (C018001, Matsunami Glass, Osaka, Japan) previously coated with 15 μg/ml poly-L-ornithine (P2533, Sigma-Aldrich) at a density ranging from 0.5 to 1 × 106 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... 4 to 5 x 104 cells/cm3 for protein harvest) onto culture plates coated with 50 µg/ml Poly-D-Lysine (PDL, Sigma Aldrich) and 20 µg/ml Cultrex™ 3D-laminin (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Cell culture plates (Denville Scientific, Inc., Plainfield, NJ, USA) were coated overnight with poly-L-lysine (50 µg/mL; Sigma-Aldrich), supplemented with 7.5 µg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... 500’000 cells per well were seeded as single cells onto new 6-well plates (6 WPs) coated with vitronectin and cultured in mTESR+ medium supplemented with thiazovivin (SML1045, Sigma Aldrich). 2 μL of each viral construct (TetO-Ngn2-P2A-puromycin ...
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bioRxiv - Molecular Biology 2024Quote: Cultured cells were scraped from tissue culture plates directly into RIPA buffer supplemented with mammalian protease inhibitor cocktail (mPIC, Millipore Sigma) and Pierce Universal Nuclease ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Microbiology 2024Quote: ... A freshly grown colony from an overnight culture was smeared onto a 96-spot steel target plate and coated with 1 μL of 70% formic acid (Sigma-Aldrich). Each spot was allowed to dry before being overlaid with 1 µL of α-cyano-4-hydroxycinnamic acid (CHCA ...
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bioRxiv - Cancer Biology 2024Quote: ... The microwell-containing culture plates were incubated with Si-PEG solution (1 mg/ml of mPEG5K-Silane (Sigma-Aldrich, JKA3037-1G) in 99% ethanol and 0.1% HCl ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times with 300 µl/well of PBS containing 0.05% Tween 20 (PBS-T) (Sigma-Aldrich, USA). Non-specific binding sites were blocked using 300 µl/well of 3% (w/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were incubated at 30 °C for the duration of the time course at “high” shake speed setting and linear shaking mode in a 96 well microtiter plate sealed with a Breathe-Easylll transparent membrane (Sigma-Aldrich). Three biological replicates were processed by growthcurver R package and doubling times were found for all strains (Sprouffske and Wagner 2016).
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bioRxiv - Microbiology 2024Quote: ... were seeded into a glass-bottom 24-well plate which had been pre-treated with poly-D-lysine hydrobromide (PDL, Sigma-Aldrich) for 1-24 h ...
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bioRxiv - Immunology 2024Quote: ... respectively) were seeded in Seahorse XF 96 designated plates using 100 µg/ml poly-D-lysine (Sigma-Aldrich, A-003-E) and assayed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cells in each culture plate were transfected with 10µg of pNL4-3 plasmid DNA complexed with 50μL PEI reagent (Sigma-Aldrich catalog # 764604), and the culture media was changed 16 hours post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... 100 µL of sample was transferred to a 96 well plate and 100 µL DMMB solution was added (0.348 mM DMMB (Sigma-Aldrich Ltd.) + 34.78 mM sodium chloride (Sigma-Aldrich Ltd. ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were stored in 24-well plates in PBS (100 mH, pH 7.4 with 0.05% Sodium Azide (Sigma-Aldrich, pH 7.4) for the IHC experiments.
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Molecular Biology 2024Quote: EBs were generated by the hanging drop method and differentiated in two dimensions (2D) in tissue culture plates coated with 1% gelatin (Sigma, G1890) in Phosphate-buffered Saline (PBS) ...
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bioRxiv - Immunology 2024Quote: ... cFibs cultured on Matrigel-coated cell culture plates were harvested and stained for Anti-Fibroblast Antibody Clone TE-7 (Millipore Sigma) and Antibody to Sarcomeric Myosin MF20 (University of Iowa Developmental Studies Hybridoma Bank ...
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bioRxiv - Microbiology 2021Quote: ... and oriT from pUC18T-mini-Tn7T-Tp-dsRedExpress were PCR amplified and introduced into a linearized pYES1L plasmid (Novagen) using the Gibson assembly method 46 (Supplementary Fig ...
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bioRxiv - Immunology 2021Quote: ... The gene sequence was amplified without the signal sequence and then PCR amplicons were ligated into the E.coli expression vector pET28a (Novagen) at the restriction sites shown in Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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bioRxiv - Microbiology 2019Quote: ... Size of the PCR products was verified by gel electrophoresis and purified using an Amicon Ultra 0.5 ml kit (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... and 2μL of purified gDNA was added to 5μL of the 2X REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich), 0.2μL 10μM forward/reverse primer mix ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with KiCqStart™SYBR®Green qPCR ReadyMix™(Sigma-Aldrich, St. Louis, MO, USA) using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... while PCRs to generate specific knock-in constructs (mAID fusions and epitope tagging) were performed with KOD polymerase (Novagen). Specific gRNA were generated using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... PCRs for genotyping of bacterial colonies after transformation were performed using the KAPA2G Fast ReadyMix kit (Sigma Aldrich, #KK5102) with custom designed primers and the following cycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Microbiology 2022Quote: The PCR products were inserted into the pET-30 Ek/LIC vector according to manufacturer’s instructions (LIC Kit, Novagen) (Table S2) ...
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bioRxiv - Microbiology 2022Quote: ... the TAR vectors were PCR amplified from pCC1BAC-his3 with KOD Xtreme Hot Start DNA polymerase (Millipore, Burlington, MA) using the construction primers (labeled “Con” ...
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bioRxiv - Developmental Biology 2022Quote: DNA fragments were released by restriction from existing plasmids or amplified by PCR using primers synthesized by Sigma-Aldrich or Eurofins ...
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bioRxiv - Plant Biology 2021Quote: ... Half of the MET3 PCR product was digested for 1h30 at 37°C with XbaI restriction enzyme (Sigma-Aldrich). ACT2 amplification was used as a control.
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bioRxiv - Plant Biology 2021Quote: ... RNA was crosslinked using UV light and hybridized with a DIG labelled probe (PCR DIG probe synthesis kit, Sigma). For detection of LbuCas13a the membrane was stripped and probed with DIG labelled Cas13a specific probe and signal detected on a Licor Odyssey imaging system (LI-COR Bioscience ...
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bioRxiv - Plant Biology 2021Quote: ... a 2.25-kb fragment containing the PHOT1 CDS was amplified by PCR with KOD hot start DNA polymerase (Novagen) using PHOT1 FW and PHOT1 RV primers (Table 1) ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was digested with restriction enzymes and then ligated into pET-17b (Merck Millipore, Burlington, MA, USA) to construct the expression vector pStSOR ...