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Citations for Millipore Sigma :
4051 - 4100 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and the recovery plate containing biofilm-recovered bacteria was covered with a breathable sealing membrane (Breathe-Easy® sealing membrane, Sigma, Australia), and incubated at 37 °C for 20 hours with shaking in microtiter plate reader (BMG ...
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bioRxiv - Bioengineering 2019Quote: ... One hundred microliters of samples were added to a black 96-well plate with equal volume of 2 mM monobromobimane (Sigma Aldrich, MO) in 50 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773) was mixed 1:1 prior to plating on the surface LB Agar plates containing 100ug/mL Ampicillin (Sigma Aldrich #A9518) and this was allowed to dry prior to the transformation reaction being plated ...
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bioRxiv - Genetics 2019Quote: ... and then passaged to a culture plate at a low density in the DMEM growth medium supplemented with 2 mM thymidine (Sigma-Aldrich T9250) for 17 hours ...
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bioRxiv - Microbiology 2021Quote: ... Lentiviral vectors were co-transduced with MD2G and PAX2 in 293T cells (5 million cells/10cm plate) with 25ul of XtremeGene9 (Millipore Sigma, #6365787001) and supernatant was harvested at 48hr and 72hr post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... age-synchronized animals at the L4 stage were collected in M9 buffer and plated on NGM plates containing 5-fluoro-2’-deoxyuridine (FUDR, Sigma, 50 μM) to prevent egg-laying (84).
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Neuroscience 2020Quote: ... 48-well MEA plates (Axion Biosystems #M768-KAP-48) were coated with 50 µg/mL poly-D-lysine (Sigma-Aldrich #P0899-50MG) in borate buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... To prevent attachment of cells all dishes/plates were coated with polyhydroxyethylmethacrylate (PolyHEMA) (12 mg/ml in 95% ethanol, Sigma-Aldrich, Germany) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Small fractions of the samples were removed and spotted on a poly(ethyleneimine)-cellulose plate (EMD Millipore – TLC PEI Cellulose F – 1.05579.0001) and let air-dried for 5-10 min ...
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bioRxiv - Cell Biology 2021Quote: Aligned electrospun PDO fibres were stretched and secured over the surface of a 24-well plate Cell Crown™ insert (Sigma-Aldrich). The whole construct was sterilised in 100% ethanol for 60 mins and rinsed five times in sterile 1 x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... dried under a stream of nitrogen and reconstituted in 25 μL of chloroform:methanol (2:1, vol/vol) and loaded on a TLC plate (Sigma Aldrich Cat #1118450001). The plate was activated using 1.8% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were back diluted to 0.1 OD600 with a final volume of 200 µL of LBM and appropriate antibiotics in a flat bottom 96 well plate (Sigma, cat#P8116) and sealed with a gas-permeable transparent seal (Sigma cat# Z380059) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the pelleted cells were resuspended in 200 µL of phosphate-buffered saline (PBS) and transferred into a black clear bottom 96-well plates (Sigma, cat#CL3603). Resuspended cells were stored at 4°C overnight to allow for sfGFP maturation [31] ...
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bioRxiv - Microbiology 2020Quote: ... TLC analysis was performed using plates coated with silica gel matrix containing a fluorescent indicator (#99876; Sigma-Aldrich, St. Louis, MO, USA) and run in the solvent system ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were surface-sterilized and imbibed for 4 days at 4°C in the dark on 0.7% (w/v) agar plates containing half-strength Murashige and Skoog (MS) media (Sigma-Aldrich; pH 5.7). Seeds were subsequently germinated and grown at 22°C under short-day conditions (8 h light/16 h dark) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended and plated on 24-well plates containing cover slips coated with 10 μg/ml poly-D-lysine (Sigma-Aldrich, Germany) at a density of 0.8×105/ml in RPMI medium (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... NCI-H2107 cells were plated at a density of 3×105 cells/ml in 12-well plates and transfected by adding dropwise 25-100 nM siRNA mixed to 20 μl X-Treme siRNA transfection reagent (Sigma-Aldrich 4476093001) in Opti-MEM™ reduced serum medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Putative transformants were transferred to PDA plates supplemented with 200 μg/mL cefotaxime and 25 μg/mL nourseothricin (Sigma-Aldrich, Missouri, USA) and absence of Mgx1LysM was confirmed with PCR using the gene-specific primers Mgx1LysM-F/Mgx1LysM-R and the primer pair with NAT-F as the forward primer that targets the nourseothricin cassette and the reverse primer Mgx1LysM-out-R targeting the downstream fragment of Mgx1LysM (Table S1).
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bioRxiv - Microbiology 2020Quote: ... Each plate included the following controls: a ‘pulldown’ of the prey being investigated using anti-FLAG antibody (Sigma Aldrich, Cat. No. F1804), to confirm the addition of functional prey ...
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bioRxiv - Cell Biology 2021Quote: ... Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787], 137 mM NaCl [Sigma-Aldrich S9888] ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and then blocked for 1 h at 37 °C with blocking buffer containing 1% BSA (Sigma-Aldrich, Taufkirchen, Germany) and 0.15M sucrose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: HUVEC plated on glass-bottom plates were rinsed in phosphate buffered saline (PBS) and fixed in 4% paraformaldehyde (Sigma-Aldrich, Rehovot, Israel) for 20 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293 cells transfected with either V2RWT or V2RT360A were seeded at a density of 0.2 million per well in a 24-well plate precoated with 0.01% poly-D-Lysine (Sigma-Aldrich; cat. no. P0899). After 24hr ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... in a 24-well plate were coated with 4 mL per plate or 500 μL per well of 50 μg/ml poly-D-lysine (PDL, Sigma, catalog #: P6407) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... WT DRG neurons were plated in 20 μg/ml PLL + 5 μg/ml Laminin coated 24 well plates at a density of 5000 cells per well in complete medium (DMEM/F12 (Sigma-Aldrich, D8437) supplemented with 1× B27 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... Vero’76 cells were seeded in DMEM with 10% FBS and 1X Pen-Strep in 96-well flat-bottom tissue culture plates (Millipore Sigma, CLS3595). The SARS-CoV-2/BetaCoV/Canada/ON-VIDO-01/2020 (Sequence available at GISAID ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated primary microglia were seeded at 500,000 cells per well (24 wells plate) and treated one day after with 2 μg/ml of Lipopolysaccharide (LPS, Sigma, #L3024-5MG) for 1h ...
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bioRxiv - Microbiology 2021Quote: ... the pegged lid was removed and washed with gentle agitation for 1 min in two new Nunc plates each containing 180 μl of phosphate buffer solution (PBS; Millipore Sigma, Canada). The pegged lid was then transferred to a new plate containing 180 μl of 0.1% crystal violet (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Neuroscience 2021Quote: Microtissues were fixed within the agarose-molded wells of the tissue culture plate with cold PFA solution (4%(w/v) paraformaldehyde, 8% (w/v) sucrose) (F79-500, Sigma-Aldrich) (S7903, Sigma-Aldrich). Microtissues were fixed for 2 hours followed by 3 PBS washes ...
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bioRxiv - Microbiology 2020Quote: ... 8% bleach and sterile water, were sown on 1/2 Murashige and Skoog (MS) salts agar (1%, w/v) plates (Sigma-Aldrich, CAD) with 1% sucrose ...
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bioRxiv - Neuroscience 2021Quote: ... cells were again dissociated with Accutase and re-plated into a 96-well plate coated with poly-L-ornithine hydrobromide (PLO, 0.1 mg/mL, Sigma-Aldrich Cat. #P3655), with 30,000 cells per well ...
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bioRxiv - Immunology 2021Quote: ... Total cell extracts were prepared and bound to Nunc MaxiSorp plates (eBioscience, Frankfurt, Germany) precoated with murine anti-FLAG mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Microbiology 2022Quote: ... each plate lid was coated with 3mL of an aqueous solution with 20% ethanol and 0.01% Triton X-100 (Sigma, cat # X100-100ML). Excess liquid was removed after 30 seconds and the lid was allowed to air-dry for 30min under a UV light for sterilization ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at >10,000 rpm to remove cellular debris and the supernatant containing extracted protein was transferred to a black flat-bottom microwell plate (Millipore Sigma, M9685). Relative fluorescence intensity of each sample was measured on a SpectraMax iD3 microplate reader (Molecular Devices ...
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bioRxiv - Bioengineering 2019Quote: ... 200μL of standard kynurenine concentrations and samples was then added to a 96 well plate followed by 100μL of 30% (w/v) trichloroacetic acid (Sigma-Aldrich, T9159-100G) to each well ...
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bioRxiv - Plant Biology 2019Quote: ... Sterilized Arabidopsis seeds were spread on agar plates containing 1⁄2 strength Murashige and Skoog (MS) medium with 1% sucrose and 0.8% agar (A1296; Sigma, St. Louis, MO, USA) and stratified at 4 °C in the dark for 2 days ...
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bioRxiv - Molecular Biology 2019Quote: ... Five thousand cells were plated in each well of a white Greiner CELLSTAR 96-well plate (Sigma-Aldrich, St-Louis, MO, USA) and transfection was performed 24 h after plating ...
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bioRxiv - Systems Biology 2020Quote: Mouse E14 embryonic stem cells (male) were routinely cultured in feeder-free conditions on gelatin-coated plates with ESGRO-2i medium (Millipore, cat:SF016-200) at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... 48-well MEA plates (Axion Biosystems #M768-KAP-48) were coated with 50 µg/mL poly-D-lysine (Sigma-Aldrich #P0899-50MG) in borate buffer ...
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bioRxiv - Immunology 2020Quote: ... 5 × 106 isolated neutrophils were cultivated in 60 cm2 plate with the addition of 100 nM PMA (Sigma-Aldrich S.r.l.,Milan, Italy) for 4 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and the cells were grown overnight at 37 °C on LB agar plates containing 50 μg/ml of kanamycin (Sigma Aldrich, USA). A single colony was selected ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We diluted the saturated culture 1:10 in 96-well plates containing 200 μL of SC[MSG] -ura -leu containing Estradiol (β-estradiol, Sigma-Aldrich) at different concentrations (0 ...
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bioRxiv - Physiology 2020Quote: ... Healthy animals were then transferred to a 96-well plate in a container filled with silica gel beads (Sigma-Aldrich, MO, USA) to produce a low humidity environment (app ...
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bioRxiv - Microbiology 2021Quote: ... the enriched cells were streaked onto a ST Gelrite plate (containing 32 g of sea salt (Sigma Aldrich, St. Louis, Missouri, USA), 1 g Na2S2O3 5H2O ...