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Citations for Millipore Sigma :
3751 - 3800 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Genomics 2021Quote: ... and CTCF-AID-eGFP19 mouse embryonic stem (mES) cells were cultured on plates pre-coated with 0.1% gelatin (Sigma-Aldrich, G1393) in Glasgow Modified Essential Medium (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Microbiology 2020Quote: Filter to remove host bacteria by transferring 200 µl (punch through pierceable tape) from each well to a 96-well filter Plate (0.45 µm) (e.g. MSHAS4510, Merck Millipore, Burlington MA US), pipette up and down a few times before extracting ...
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bioRxiv - Immunology 2021Quote: KP bacterial load was determined by plating ten-fold serial dilutions of the lung homogenates onto MacConkey agar plates (Sigma-Aldrich). The plates were incubated at 37°C overnight before bacterial CFUs were determined by colony counts.
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bioRxiv - Immunology 2020Quote: ... 50 µl of beads and 50 µl of diluted plasma was added to 96-well microtiter plates (MSBVS 1210, Millipore, USA) pre-wetted with ABE ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Immunology 2021Quote: ... 6000 OVCAR3 cells were seeded on the wells of a flat-bottom 96-well plate in RPMI containing L-glutamine (R8758, Sigma-Aldrich), supplemented with penicillin/streptomycin (100 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... albicans homozygous deletion library [44] arrayed in 96 colony format on YPD plates (145×20 mm) using a replica plater (Sigma #R2508). Control N/S plates were grown at 30 °C for 48 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... a single adult male or female worm was cultured in one well of a standard 24-well cell culture plate (Sigma-Aldrich). For infective L3 stage worms ...
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bioRxiv - Neuroscience 2020Quote: ... a single organoid per well was transferred on 6 well plates on day 77 and 120 in Diff2 medium with 20 ng/ml LPS serotype O111:B4 (Sigma # L2630) and incubated for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... These parasites were cultured (10 sex-separated adults or five adult pairs per well in a 48 well tissue culture plate) at 37°C in DMEM (Sigma-Aldrich) supplemented with 10% foetal calf serum ...
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bioRxiv - Molecular Biology 2021Quote: ... The MIC was determined using the broth dilution method on 96-well plate in cation-adjusted Mueller-Hinton broth 2 (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... Monocytes were seeded in 96 well plates at 1.5×106 cells/ml in the presence or absence of 10ng/ml of lipopolysaccharide (LPS; Sigma-Aldrich, USA). The aqueous extracts from the plants were added to the stimulated or unstimulated cells at three different concentrations of 50 ...
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bioRxiv - Immunology 2020Quote: ... Analytical thin-layer chromatography (TLC) was carried out on 250 μm silica gel 60G plates with fluorescent indicator F254 (EMD Millipore). Plates were visualized with UV light and treated with p-anisaldehyde ...
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bioRxiv - Microbiology 2021Quote: ... and then seeded at a 1:4 dome-to-well ratio in a 96-well plate coated in 100 µL of 34 µg/mL human collagen IV (Sigma C5533). Cells were fed every two days and were used for experiments after they were fully confluent (4-5 days) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... each piece of frozen tissue was placed into one well of a 6-well plate with salt-Tris buffer containing 146 mM NaCl (Cat#S6546-1L, Sigma-Aldrich), 1 mM CaCl2 (Cat#97062-820 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were grown overnight to exponential phase and five serial dilutions (1/4) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were incubated at the relevant temperature ...
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bioRxiv - Immunology 2022Quote: ... 10-15 µg of proteins were loaded onto a 96-well plate with an activated and primed polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma). Proteins adsorbed to the membrane were trypsinized by incubation with the protease for 2 hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The coating solution was then flicked off and the plate was washed three times with PBS containing 0.1% Tween® 20 (Sigma Aldrich). Plates were then blocked with PBS containing 10% FCS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into mouse hypothalamus Pomc neuronal cells (mHypoA-POMC/GFP-1 from Sanbio #CLU500) or human astrocytes (CCF-STTG, ATCC) grown in 96-well plates of 132 using X-tremeGene (Sigma, 6366244001) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Brains and spinal cords were dissected and placed in 6 well plate with cold dissection buffer (HBSS no cations with 5ug/ml actinomycin, 10uM triptolide, and 27ug/ml anisomycin (Sigma A9789)) protected from light ...
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bioRxiv - Immunology 2022Quote: ... the primary oligodendrocytes were plated at a density of 100,000 cells per 24 well plate on 10% PLL (Sigma, P4832-50ML) and 1.5% laminin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... the MEA plate was treated by removing half of the medium and replacing with medium containing 100 μM 4-Aminopyridine (275875, Sigma-Aldrich) at 2X ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures in the plates were then transferred by the liquid handler into glass-bottom 384-well microscope plates (Matrical Bioscience) coated with concanavalin A (Sigma-Aldrich). After 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Developmental Biology 2022Quote: NPCs cells were plated into Matrigel-coated 10mm2 plate and treated for 3 days with SAHA (20 nM, Sigma-Aldrich, in DMSO (0.05%, Sigma-Aldrich)) in culture media before performing western blot analysis ...
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bioRxiv - Genomics 2022Quote: ... cells were plated in a round bottom 96-well plate in complete RPMI medium containing PMA (Sigma; final concentration 100ng/mL) and Ionomycin (Sigma ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...
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bioRxiv - Genetics 2022Quote: ... 30 adult worms were dropped in 10 μl S-basal in a well of a Corning Costar® 12-well plate and covered with 2.3 ml of 26% w/v Pluronic F-127 (Sigma-Aldrich) at 14 °C ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were harvested by centrifuging for 5 minutes at 12,000 rpm and resuspended in LB and transferred to wells of 12-well plate containing glass coverslips coated with polylysine (Sigma-Aldrich). The plate was incubated at 37°C for 48 hours after which the biofilm specimens were prepared for electron microscopy as described by Ochmcke et al ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
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bioRxiv - Immunology 2022Quote: ... 5×105 reporter-expressing Jurkat cells were plated in a 96-well plate and cultured for 24 hours with complete RPMI supplemented with the indicated (10ng PMA (Sigma-Aldrich) / 200ng ionomycin (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... seedlings were grown vertically on half-strength MS plates supplemented with 1% (w/v) sucrose and 0.5% (w/v) Phytagel (Sigma-Aldrich, P8169). Five-day-old seedlings were moved to liquid half-strength MS medium containing 2 μM synthetic RALF1 peptide (Sangon Biotech Co ...
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bioRxiv - Microbiology 2022Quote: ... The culture plates were prepared by coating with 0.001% poly-L-lysine solution (Millipore Sigma, P4707, diluted in water 1:10) for plastic surfaces and 100 µg/ml poly-L-lysine hydrobromide (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... A yeast colony was picked from a YSM-Trp plate and resuspended in 100 µl of 5% w/v Chelex-100 (Sigma-Aldrich). Half the sample volume of acid washed glass beads were added and the sample vortexed at maximum speed for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... faecalis wild-type OG1RF and OG1RF ΔmptD were sub-cultured to OD600 of 0.01 in a 24-well microtiter plate containing 1 mL of Tryptone Soya Broth without dextrose (TSBd, Sigma-Aldrich, USA) supplemented with or without mannose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 5 isolated morphologically normal colonies selected from the agar plates were suspended in cation adjusted Mueller Hinton broth (CAMHB, Sigma-Aldrich). The bacterial suspensions were diluted with normal saline to achieve a turbidity equivalent to a 0.5 McFarland standard (latex ...
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bioRxiv - Microbiology 2022Quote: ... Equal numbers of cells were lysed in 96-well plates using 100 μl lysis buffer (1% Triton X-100 (Sigma Aldrich), 25 mM Tricine (VWR) ...
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bioRxiv - Neuroscience 2022Quote: ... 15,000 neurons were plated per well in 24-well plates on coverslips coated with poly-DL-ornithine (Sigma-Aldrich Cat#P0671) and laminin (Corning Cat#354232 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Each well of a cell capture plate contains a small 50 nl droplet of barcoded primers and 10 μl of mineral oil (Sigma M8410). After sorting ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Neuroscience 2023Quote: ... 4.000 hiPSCs per line were plated in matrigel-coated 96W plates and 2 days later and incubated with 0.5 µg/ml of cycloheximide (CHX, Sigma Aldrich, C1988) for the indicated time followed by fixation ...