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Citations for Millipore Sigma :
4151 - 4200 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 1.5 x103/cm2 on 13 mm coverslips in 24 well-plates and cultured in DMEM containing 2% with human AB serum (HS; Sigma Aldrich, H4522) in a 5% humidified CO2 atmosphere at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... cell culture medium from the plates was replaced by 175 µL/well of pre-warmed low-buffered serum-free minimal DMEM (D5030, SIGMA-Aldrich, USA) medium supplemented with 1 mM pyruvate ...
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bioRxiv - Genetics 2024Quote: ... and placed individually into wells of a 96 well tissue culture plate (Falcon: FAL353072) that contained 700uL of 2% agar (Sigma Aldrich: A7002). Before flies awoke ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used an isoamyl alcohol extraction protocol outlined in Harborne (1984) and separated pigments using TLC on Cellulose F glass plates (Millipore, Burlington, MA). We compared extracted anthocyanidin pigments to a multi-anthocyanidin standard solution containing pelargonidin ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Immunology 2024Quote: ... plates were again washed three times with PBST prior to the addition of 100 μL of o-phenylenediamine dihydrochloride (Sigma-Aldrich; OPD). Plates were allowed to develop for 10 minutes after which the reaction was stopped via the addition of 50 μL of 3M hydrochloric acid (HCl) ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Biochemistry 2024Quote: ... the insoluble fraction is removed by transferring TPP-PISA treated samples to a hydrophilic polytetrafluoroethylene 96-well filter plate with a 0.45 μm membrane pore size (MSRLN0410, Millipore, Burlington, Massachusetts, USA). The filter plate is pre-wetted with 100 μl of PBS and centrifuged for 2 minutes at 500xG ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Plant Biology 2023Quote: Overnight cultures were resuspended to OD600=0.05 in low-salt LB and 100 µL were supplemented with 100 µL of purified effector proteins or culture filtrates to a final OD600=0.025 in clear 96 well flat-bottom polystyrene tissue culture plates (Greiner CELLSTAR®, Sigma, Burlington, U.S.A). Culture plates were incubated in a CLARIOstar plate reader (BMG LABTECH ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were then seeded in 6-well plates and cultured for 7 to 14 days in the presence of 60 μM 6-thioguanine (Sigma-Aldrich, A4882) [23] ...
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bioRxiv - Immunology 2022Quote: ... the solution was removed and the plate was washed 2X with 200 µL wash solution and 100 μL TMB solution (Sigma, #T0440-100ML) is added ...
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bioRxiv - Immunology 2022Quote: ... and the plate was blocked with 200 µL blocking solution (1% BSA [Sigma, #A9418-5G], 0.05% Tween [Sigma, #P9416-100ML], in PBS). The solution was removed and sample or standard curve (made with mouse IFN-β [PBL Biomedical Laboratories ...
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bioRxiv - Immunology 2023Quote: ACE2-HEK293 ΔRIG-I cells were seeded at 5×10E4 cells per well in 24-well plates that had been treated with Poly-D-lysine hydrobromide (Sigma, P6407-5MG) and reverse transfected with 50 nM/well of control (CTRL ...
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bioRxiv - Immunology 2022Quote: ... serial dilution of splenocyte suspensions of □MT chimeric mice were added to NP20-BSA-coated MultiScreen HA mixed cellulose ester plates (Millipore, Watford, UK) previously washed and blocked with IMDM (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Neuroscience 2023Quote: ... These droplets were allowed to gel at 37°C for 25 minutes and were subsequently removed from the Parafilm and transferred to and maintained in low-attachment 24-well plates (Sigma, cat. #CLS3473) containing induction medium for the following 5 days ...
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bioRxiv - Plant Biology 2023Quote: ... the remained residue was dissolved in 2 ml EA and loaded onto a few TLC silica gel 60 plates (2 mm thickness, 20 cm × 20 cm, EMD Millipore, USA). The TLC plates were developed with a solvent mixture consisting of water ...
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bioRxiv - Neuroscience 2023Quote: ... NSCs (5 x 106) were seeded in NEM medium onto 100 mm plates coated with 100 μg/mL polyornithine (PLO) (Sigma Aldrich, P3655) and 20 μg/mL laminin ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... and adjusted to a proper concentration using antibiotics-free culture medium before seeding in plates which precoated with 10 μg/mL FN1 (Sigma, #F1141-1MG). Synthetic Silencer Select siRNAs against human NFATC4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 individual peptides (2 μg/ml) or blank control (complete medium with DMSO) onto sterile nitrocellulose MSIP 96-well plates (Millipore, Bedford, MA) coated with capture antibodies against mouse IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... ELISPOT plates were washed three times with sterile 1XPBS and blocked for two hours at 37°C using RPMI 1640 (Sigma, Cat. #R0883) supplemented with 1% L-glutamine (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... pylori strains were grown on blood agar plates (Oxoid blood agar plates base II) supplemented with 10% horse blood (Oxoid) and the following antibiotics (all purchased from SIGMA-Aldrich, USA): amphotericin B (4 mg/L) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS in fibronectin-coated cell culture flasks and seeded into plates coated with 20 μg/mL Poly-L-Lysine (Sigma-Aldrich, #9155) before experiments ...
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Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... FLAG-Ago2 was immunoprecipitated from 800 µg of tissue lysate using an Anti-FLAG M2 coated 96-well plates (P2983, Sigma-Aldrich, Ireland) as per the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... two biological replicates were transferred into SC dropout media in a 96 well plate (Fisherbrand, 12566611) sealed with gas-permeable membranes (Sigma-Aldrich, Z763624) and grown overnight shaking at 1000 rpm at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... The ligated fragment was transformed into the first-step constructed mutant strain and selected by using plates containing p-Cl-phe (4 mg/mL, Sigma, United States). Finally ...
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bioRxiv - Cancer Biology 2023Quote: Total cell lysates were extracted from adherent cells by incubating the cells for 10 minutes on plate in ice cold cell lysis buffer (150 mM NaCl (Sigma-Aldrich: S3014); 1 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... After lipid extraction against organic solvent — CHCl3/MeOH 2:1 (vol/vol) — samples were spotted on silica plates (Sigma-Aldrich, Steinheim, Germany) with the automatic TLC sampler 4 (CAMAG ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 × 104 cells were seeded per well in 24-well MatTek glass-bottom plates (P24G-1.5-10-F) coated with 0.1 mg/ml poly-D-lysine (Sigma; 30,000-70,000 mol wt). Cultures were maintained in organoid base medium without small molecules ...
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bioRxiv - Plant Biology 2023Quote: ... An aliquot of each sample was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, Burlington, USA), using a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water at a ratio of 80:40:10:10:10 (v/v) ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... 30 µL of each sample was transferred into a clear-bottom plate (two technical replicates per biological sample) together with 100 µL of free glycerol reagent (Sigma‒Aldrich, F6428) and incubated at 37°C for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: A feeder layer of inactivated murine embryonic fibroblast cells was cultured in 6-well plates on gelatin (0.1%, Sigma-Aldrich G7041-100G) at a seeding density of 1.6 x 104 cells/cm2 in base medium consisting of DMEM (1x ...
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bioRxiv - Developmental Biology 2023Quote: ... a feeder layer of inactive murine embryonic fibroblast cells was cultured in 6-well plates coated with 0.1% gelatin (Sigma-Aldrich G7041-100G) at a density of 1.6 x 104 cells/cm2 in a base medium containing DMEM (1x ...
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bioRxiv - Neuroscience 2023Quote: ... cells were sorted via flow-cytometry based on GFP signal intensity to yield GFP-positive neurons that were plated on PDL/laminin-coated plates (Sigma, Life technologies). Neurons were maintained in Neurobasal medium (Life Technologies ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... at room temperature for 30 minutes in a 96-well flat-bottom plate pre-coated with poly-D-lysine (Sigma-Aldrich #P7280). An Axio Observer 7 microscope was used to image 10% of each well immediately after staining ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVECs on gelatin-coated plates were incubated with gcEVs labeled with the fluorescent dye PKH-26 (Sigma-Aldrich, 10 min at RT). After washing ...
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bioRxiv - Cancer Biology 2023Quote: ... and seeded at 1 x 105 in a 24 well plate and then pulsed with 1ng/mL OVA peptide (Sigma, #S7951-01mg) for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ARPE19 cells were plated at 40,000 cells per well on a Seahorse XF platform compatible 96-well plate pre-coated with poly-D-lysine (Sigma Aldrich, USA) and allowed to adhere and grow for 24 h28 ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial suspension was adjusted to obtain an OD600 of 0.05 into 96-well microtiter plates to apply final volume of 200 µl per well-adjusted with TSA medium (Sigma-Aldrich, India) supplemented with 20% (vol/vol ...
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bioRxiv - Cell Biology 2023Quote: Zoospores were adhered for 5 min to plasma cleaned 96-well plates (MatriPlate, Cat. No. MGB096-1-2-LG-L) coated with 0.5 mg/mL Concanavalin A (Sigma, Cat. No. C2010). We fixed the cells in 4% PFA and 50 mM sodium cacodylate ...
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bioRxiv - Immunology 2023Quote: ... Plates were then blocked for 6 h with PBST (PBS with 0.01% Tween-20) containing 2% BSA (Sigma-Aldrich; cat. no. 10735094001) at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Plasma samples were briefly centrifuged at 10,000 × g for 10 min or filtered through a 1.2-µm filter plate (Millipore Sigma, Cat. No. MSBVN1210) prior to use ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts were then placed into culture plate wells containing Neurobasal-A medium supplemented with 25 µM L-Glutamic acid (Sigma-Aldrich, G8415), 2 mM Glutamax (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Chemically defined medium (CDM) editing plates supplemented with 10 ng/ml BDNF and 10 ng/ml GDNF on poly-L-ornithine (Sigma-Aldrich, #P4957) and laminin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 2.5 x 105 splenocytes from OT-1 mice were seeded in one ml of complete media in 24 well plates and 0.5µg/ml of peptide (Sigma-Aldrich, cat. number S7951). After 48 hours blasts were expanded with 100 U/ml of rmIL-2 in fresh media and harvested on day four or six after initial stimulation.
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bioRxiv - Cell Biology 2023Quote: ... In the meantime, the mold placed in a 24-well plate (costar, #3527) and was coated with trichloro silane (Sigma-Aldrich; #448931) in another desiccator under vacuum system for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were assayed on a GloMax® Explorer Multimode Microplate Reader after incubation with Resazurin (16 µg ml-1; Sigma-Aldrich) for 4- 6 h.