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Citations for Millipore Sigma :
3851 - 3900 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and/or eGFP fluorescence (Ex/Em: 488 nm / 510 nm) was measured in clear 96 well plates sealed with a Breathe-Easy® membrane (Sigma) with linear shaking at 37°C for 48 h using a Spark 10M plate reader (Tecan).
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bioRxiv - Systems Biology 2023Quote: ... set to “high” shake speed and linear shaking mode in a 24 well microtiter dish plate sealed with a Breathe-EasyⓇ transparent membrane (Sigma-Aldrich). For the data in Supplementary Figure 9 ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-L-Lysine and Laminin coating of 24-well plates was performed by 48 h incubation at 37°C using Tissue Culture water (Sigma, W3500) supplemented with 1 μg/ml Poly-L-Lysine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich). Before freezing the cells were incubated with ROCK inhibitor Y-27632 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 20,000 tumor cells were plated in Poly(2-hydroxyethyl methacrylate) (PolyHema)-coated 96-well flat bottom plates (Sigma-Aldrich #P3932-25G). Tumor cells were well mixed to ensure single-cell suspension prior to plating ...
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bioRxiv - Cancer Biology 2023Quote: ... The HLA complexes bound to the beads were then directly eluted into preconditioned Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters, 186002321) using 1% trifluoroacetic acid (TFA; Sigma Aldrich). After two washes of 1mL 0.1% TFA ...
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bioRxiv - Microbiology 2023Quote: ... pneumophila strains were routinely grown from frozen stocks stored at-80 °C on CYE (ACES-buffered charcoal yeast extract) agar plates (Sigma-Aldrich) adjusted to pH 6.90 with KOH and supplemented with 0.25 g/L L-cysteine and 0.4 g/L ferric pyrophosphate and incubated at 37 °C for 72 hours ...
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bioRxiv - Genetics 2023Quote: ... Tissues were then transferred into an enzymatic digestion buffer (200 µL, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... A whole colony was picked from this plate and inoculated into a preculture of 10mL HS media with 0.2% v/v cellulase (Sigma Aldrich C2730) in a 125mL flask ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded at a density of 15,000 cells per well on a 96-well low-attachment U-bottom plate (Sigma, cat. #CLS7007) in mTeSR plus 10 μM ROCK inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... for co-immunoprecipitation analysis and into 96-well cell culture plates (3×104 cells/well) coated with 0.01% poly-L-lysine (Sigma-Aldrich, #P9404) for ELISA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature sensory neurons were dissociated and seeded as single cells onto plates coated with poly-L-ornithine hydrobromide (20 µg/mL, P3655, Sigma-Aldrich), laminin (10 µg/mL ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...
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bioRxiv - Cell Biology 2023Quote: ... cells were serially diluted 10-fold and 2 μl of each dilution was spotted on plates containing 5 mM hydroxyurea (Sigma-Aldrich) or on YEPD and exposed to UV with a dose of 200 J/m2 ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 CHO cells were seeded per well in a 24-well plate in supplemented Minimal Essential Medium (MEM with 10 % FBS, 2 % non-essential amino acid solution (Sigma Aldrich), 1 % GlutaMAX-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were grown to 70–80% confluency (optionally with 1 μg/ml doxycycline for 24–48 h) in 24- or 12-well plates and treated with colchicine (10 μM, Sigma PRH1764), combretastatin A4 (Selleckchem S7783) ...
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bioRxiv - Immunology 2022Quote: ... the PBMC and CD4+ cell fractions were then added to either HCMV-infected or uninfected autologous primary dermal fibroblasts at E:T ratios of 10:1 in 24-well plates in Roswell Park Memorial Institute (RPMI)-1640 media (Sigma-Aldrich, UK) supplemented with 10% FBS and 100 units/ml of penicillin/streptomycin ...
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bioRxiv - Developmental Biology 2023Quote: ... while all exit and differentiation experiments were performed in plates coated with 10μg/ml laminin in PBS for at least 30 min at 37°C (Sigma Millipore CC095). For all experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... 3.0 × 103 iMeLCs were plated into ulta-low attachment U-bottom 96-well plate (PHCBI, MS-9096UZ) in GK15 supplemented with: 10 ng/ml LIF (Millipore, LIF1005), 200 ng/ml of BMP4 (314-BP-010 ...
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bioRxiv - Immunology 2023Quote: ... IDE12 cells were also grown to confluency in T25 flasks and either seeded at 1x106 cells/well in 6-well plates (Millipore Sigma) or 3x105 cells/well in 24-well plates (Corning) ...
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bioRxiv - Biochemistry 2023Quote: ... 50 µL of the culture was spread onto pre-warmed plates containing 100 µg/mL ampicillin (Sigma-Aldrich, St. Louis, MO) for antibiotic selection and incubated at 37 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: Worms at the L4s stage from strains expressing ChRimson were picked onto an NGM/OP50 plate coated with a 100 µM concentration of all-Trans Retinal (Sigma-Aldrich, Cat No. ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... 1×105 cells were plated on a 12-well cell culture multiwell plate containing poly-D lysine-coated coverslips (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were cultured at 37°C with agitation either in broth or on agar plates with Luria-Bertani (Becton-Dickinson) with appropriate antibiotics (Sigma-Aldrich) added for maintaining plasmids if needed ...
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bioRxiv - Microbiology 2023Quote: ... Enterococcal strains were cultured at 37°C with agitation either in broth or on agar plates with Brain Heart Infusion or Tryptic Soy Broth (Becton-Dickinson) with appropriate antibiotics (Sigma-Aldrich) added for maintaining plasmids if needed ...
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bioRxiv - Neuroscience 2023Quote: ... and neurons plated in NPM onto 24-well plates (5 x 104 cells/well) precoated with poly-L-lysine hydrobromide (Sigma P2636) (1 mg/mL for glass coverslips or 0.1 mg/mL for plasticware ...
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bioRxiv - Bioengineering 2023Quote: ... was then added to the construct and the posts containing the muscle construct was removed from the agarose mould and placed into a new 12-well plate with myoblast medium containing 33 µg/µl aprotinin (Sigma, A3428) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Cells cultured in the 24-well plates in ESTEM-HE medium were treated with 100 nM vitaminD3 (D1530-10UG, Sigma-Aldrich)or 40 μM β-naphthoflavone (N3633-1G ...
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bioRxiv - Cancer Biology 2023Quote: The 12-well plates used for tumor sphere formation were pre-treated with 12 mg/ml poly HEMA (SIGMA, Catalog # P3932) and dried on a clean bench ...
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bioRxiv - Cancer Biology 2023Quote: ... the 96-well collection plate was submitted to the Molecular Biology Core Facilities at Dana-Farber Cancer Institute where the collection plate was dried down using a permeable membrane (Sigma, A9924) and resuspended in 10 µl of molecular biology grade nuclease-free water ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 target tumour cells were seeded in 6 well plates and infected with lentiviruses + polybrene (8 µg/mL) before selection of transduced cells using 4ug/mL puromycin (Sigma-Aldrich).
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... and cells were pipetted into individual wells of a 96-well white opaque optical plate pre-loaded with 50 units of HRP (Sigma-Aldrich). This was introduced into a warmed (37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in Feeder-free on pre-coated plates with 0.5% growth factor-reduced Matrigel (CORNING 356231) (vol/vol in PBS with MgCl2/CaCl2, Sigma-Aldrich D8662) in E8 medium (made in-house according to Chen et al. ...
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bioRxiv - Cell Biology 2023Quote: Human neuroblastoma SH-SY5Y cells (1 ×105) were seeded into a 24-well plate coated with poly-D-lysine (p6407, Sigma-Aldrich) one day before pseudo-lentiviral particle transduction ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded according to FUJIFILM Cellular Dynamics user protocol onto the 96-well plates coated with poly-L-ornithine (P3655, Sigma-Aldrich) and laminin (L2020 ...
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bioRxiv - Microbiology 2023Quote: ... Βecton Dickinson Company) broth or agar plates or TY medium (48) in the presence of 1-5 µg/mL thiamphenicol (Sigma-Aldrich) or anhydrotetracycline (ATc ...
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bioRxiv - Neuroscience 2023Quote: ... 30µl of sample was transferred into a 96-well plate and added 100µl of Hexokinase enzyme reagent (HK) (Sigma-Aldrich, UK), and then mixed and incubated at room temperature for 15mins (Fig ...
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bioRxiv - Immunology 2023Quote: ... Liquid cultures were prepared from bacterial plates before washing in PBS and diluting in 2% (w/v) polyvinylpyrrolidone40 (PVP40, Sigma-Aldrich) for injection as described previously (Benard et al ...
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bioRxiv - Plant Biology 2023Quote: Individual 7-day-old Arabidopsis seedlings were transferred to white 96-well plates containing 200 µl reconstitution buffer (2.5 mM MES pH 5.7 (Sigma-Aldrich, Taufkirchen, Germany), 10 mM CaCl2 (Roth ...
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bioRxiv - Bioengineering 2022Quote: ... The mix for the tumour mass was then set into 96-well plates (Corning® Costar®, Sigma-Aldrich, Dorset, UK) and incubated in 37°C for 15 min to allow crosslinking ...
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bioRxiv - Bioengineering 2022Quote: ... 650 μl of the stromal gel mix was cast on 24-well plate (Corning® Costar ®, Sigma-Aldrich, Dorset, UK). The tumour mass was placed in the middle of the first stromal layer and covered by a second stromal layer of 650 μl of the stromal gel mix.
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bioRxiv - Cell Biology 2023Quote: ... in buffer Carbonate (7,13g/L NaHCO3 + 1,59 g/L Na2CO3 pH 9,5) was used to immobilize 96 well plaques (Nunc Immuno™ MicroWell™ 96 well solid plates, Sigma), o.n ...
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bioRxiv - Cancer Biology 2023Quote: ... and GSC #161) were obtained from the Mayo Clinic [44] and cultured on laminin-coated plates (1–2 μg/cm2) (Sigma #L2020) in StemPro neural stem cell (NSC ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of cells were sonicated for 5 s before being loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Y04C, Millipore) connected to the ONIX2 (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then inoculated to the upper chamber of the transwell plate while in lower chamber DMEM supplemented with 0.5% FBS and 40μg/ml collagen I (Sigma-Aldrich, Missouri, USA) was added ...
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bioRxiv - Cancer Biology 2023Quote: Cells seeded in six-well plates were cultured overnight with 2 mL fresh medium with or without 25 mM D-glucose-1,2-[13C2] (Sigma-Aldrich, 453188) before treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... 30 μl was transferred to a 96-well plate and incubated with 100 μl of Free Glycerol Reagent (Sigma F6428-40ML) for 5 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Peanut lectin-coated chamber slides or plates were prepared by incubating peanut lectin (0.5 mg/mL) (Sigma-Aldrich, St. Louis, MO) suspended in water in chamber slide wells for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells of BC tissues were concentrated in six-well plates coated with 2% poly-2-hydroxyethyl methacrylate (Sigma-Aldrich) at a density of 1×104 cells/ml in serum-free DMEM/F12 (Gibco ...