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Citations for Millipore Sigma :
3801 - 3850 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... neutrophils were plated in 24-well plates containing glass coverslips covered with 0.01% poly-L-lysine solution (Sigma-Aldrich; cat. P8920), fixed with PFA 4% at RT for 10 min ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Neuroscience 2022Quote: ... 20 young adult animals were placed in the center of an unseeded NGM agar plate supplemented with 1 mM aldicarb (Sigma-Aldrich). Animals were scored every 10 or 20 min as paralyzed if they did not move when gently touched with a platinum wire on the tail ...
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bioRxiv - Neuroscience 2022Quote: ... containing the corresponding cell suspension for 9000 cells/well were plated for each cell line using low attachment 96-well plates (Sigma CLS7007). Briefly ...
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3MDR, a microcomputer-controlled visual stimulation device for psychotherapy-like treatments of micebioRxiv - Neuroscience 2022Quote: ... height 20 cm) on a smooth acrylic glass base plate in no-visible-light conditions and scented with 1,4-cineol (Sigma Aldrich, USA). Context C consists of a square chamber (16 cm * 16.5 cm * 25 cm ...
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bioRxiv - Molecular Biology 2022Quote: ... were grown in TYG medium (with/without kanamycin; 8 µg/ml) in 96 well microtiter plates after washing with PBS (Nunclon; Sigma-Aldrich). Optical density at 600nm was measured to examine the growth in replicates at 32 °C for 18 h using Synergy H1 Hybrid multi-mode microplate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured on poly-D-lysine-coated coverslips in 24-well plates and were differentiated by adding 10 µM all-trans retinoic acid (Sigma; R2625) on day 2 and conducting a media exchange to brain-derived neurotrophic factor (BDNF)-containing media (DMEM with 50 ng/ml BDNF ...
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bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
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bioRxiv - Microbiology 2022Quote: ... Aerobic assays were incubated in a 28°C shaker at 225 rpm in 96-well plates sealed with Breathe-Easy membrane (Sigma-Aldrich). All other O2 conditions were generated in a gas-controlled chamber (Ruskinn Invivo2400 ...
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bioRxiv - Cell Biology 2024Quote: ... 1×105 ESCs were placed per well of a 12-well plate coated with 16.7 μg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) in N2B27 medium containing 20 ng/mL Activin A (Peprotech ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells cultured in 96-well plates (50000 cells/well) were starved for 30 min in DMEM supplemented with 500 µM IBMX (Sigma Aldrich). To induce de novo synthesis of cAMP ...
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bioRxiv - Systems Biology 2024Quote: ... either resuspended from a plate or from liquid culture and was further purified by spot dialysis on a nitrocellulose filter (Millipore VSWP02500) against 0.1 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Each well of a cell capture plate contains a small 50 nl droplet of barcoded primers and 10 µl of mineral oil (Sigma M8410). After sorting ...
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bioRxiv - Biochemistry 2023Quote: ... Two hundred microliters of a cell suspension at OD 0.04 were loaded in the well of a 96-well plate (PS96B-G175, SwissCI) coated previously with Concanavalin A (L7647, Sigma-Aldrich). The imaging was performed on a Nikon Ti2 inverted microscope enclosed in a temperature incubation chamber set at 30°C and controlled by micro-manager35 ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL x 3 from each sample was add to a well in Corning transparent 96-well plate (cat#CLS2585 from Millipore Sigma) and absorbance was measured at 690 nm in a Tecan Spark Plate Reader ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... KD-LX2 and Scr-LX2 cells were seeded in 6-well-plates (4x104 cells/well) for 48hrs before being stained with Nile Red (Sigma-Aldrich) and evaluated with densitometry by ImageJ software to measure lipid content [24] ...
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bioRxiv - Physiology 2024Quote: ... were spread onto NGM plates containing 100 μg/ml ampicillin and 1 mM isopropyl 1-thio-β-Dgalactopyranoside (IPTG, 420322, Millipore) for 24 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... and plated at single-cell onto matrigel coated plates with induction media containing 10 μM Y-27632 and 2 μg/mL doxycycline (Sigma D9891) as previously described40 ...
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bioRxiv - Microbiology 2024Quote: ... cells were diluted (1:10000) into new microtiter plates with fresh TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK). The plates were sealed with a gas permeable membrane (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 24 h in 24-well plates in complete culture media supplemented with 50 ng/mL PMA and 1 μg/mL ionomycin (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Two hundred thousand cells per well were seeded into a 12-well plate pre-coated with poly-D-lysine (5 μg/ml) in 0.1 M borate buffer (Sigma-Aldrich, #P7280). Cultures were grown in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Spinfection plates were pre-coated using 500 uL of PBS with 10 uG/mL of purified human plasma fibronectin (Sigma-Aldrich) and incubated at 37°C several hours prior to transduction ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Neuroscience 2024Quote: Young adult worms were harvested from standard NGM plates and subjected to an overnight (∼16 hours) treatment by placing them on NGM plates supplemented with 15 mM Lithium Chloride (LiCl, Catalogue No. L9650, Sigma-Aldrich), lithium treatment protocol was adapted from Meisel & Kim et al. ...
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bioRxiv - Cancer Biology 2024Quote: 0.5 to 1 million cells were plated into 6-well plate and treated with 0.5 or 1 mg/ml Dox (Sigma-Aldrich #D9891) to induce FGFR4 overexpression or knockdown for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... were inserted into 24-well culture plates (Falcon) and coated with 100 μg/mL poly-D-lysine solution (Sigma-Aldrich #1804) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... The liquid handler was then used to transfer strains into glass-bottom 384-well microscope plates (Brooks Bioscience) coated with Concanavalin A (Sigma-Aldrich) to allow cell adhesion ...
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bioRxiv - Cell Biology 2024Quote: ... were produced by transfecting HEK293T cells plated at a density of 1 × 107 cells/15 cm plate one day prior to transfection using polyethyleneimine (Linear PEI, MW 25 kDa, Sigma-Aldrich) as the transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: Cells were incubated at 37 °C for 6 hr to allow adhesion and spreading onto a cell culture plate before the addition of 15 μM NCT503 (#19718, Cayman) or 10 μM WQ2101 (#SML1970, Millipore Sigma). After 48-hr treatment with the drug ...
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bioRxiv - Molecular Biology 2024Quote: ... for differentiation induction 100.000 cells were plated in a 6-well plate and maintained in complete media supplemented with 20μM retinoic acid (Sigma-Aldrich, R2625) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells were seeded on 0.5 mg/ml poly-D-lysine-coated sterile 12-mm diameter coverslips or aligned 700-nm diameter nanofibers in 24 well plates (Sigma, Z694533). Growth medium was changed on days in vitro (DIV ...
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bioRxiv - Cell Biology 2024Quote: MSCs were seeded overnight on 24 or 6 well plates in normal α-MEM supplemented with 10% fetal bovine serum (FBS, Sigma), 2 mM glutamine ...
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bioRxiv - Genomics 2024Quote: ... 1:1000 and 1:10,000 dilutions of the outgrowth were plated on LB plates carrying 50 μg/mL kanamycin (LB+Kan50, Sigma-Aldrich #K1377) and incubated at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: The experimental plate for HepG2/C3A cells was prepared by coating with 100 µg/mL of rat tail collagen (Sigma-Aldrich) and 0.02 M glacial acetic acid in PBS for a minimum of 2h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... was added to a well of a 24 well plate and a 6.5 mm Transwell® Polycarbonate Membrane insert with a 5.0 µm pore (Costar #3421/Sigma # CLS3421-48EA) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... For the optogenetics: ZX299 transgenic worms were cultivated on agar plates with OP50 bacteria in the presence of all-trans retinal (Sigma-Aldrich) (Nagel et al. ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The supernatant (approximately 850 µL) was then transferred into separate wells of a HybridSPE-plus 96 well filter plate (Sigma, Japan).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The supernatant (approximately 850 µL) was then transferred into separate wells of a lipid HybridSPE-plus 96 well filter plate (Sigma, Japan) taking care not to disturb the pellet.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10 mg/mL, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... a 384-well hanging-drop (HD) plate custom designed with injection molding (Xcentric) was soaked in 0.1% solution of Pluronic F108 (Sigma-Aldrich, 542342) in distilled water (diH2O ...
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bioRxiv - Biochemistry 2023Quote: ... Sigma FastTM p-nitrophenyl phosphate substrate was reconstituted in sterile Milli-Q water and 180 μl of it was added to each well of the 96 well plate and incubated at room temperature in the dark for up to 24h (Sigma-Aldrich). Plates were read at 405 nm on an Emax precision microplate reader (Molecular Devices) ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...