Labshake search
Citations for Millipore Sigma :
3901 - 3950 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: hα7-nAChR/m5-HT3A chimera-transfected Vero cells (105 cells/mL) were coated onto 96-well plates pretreated with poly-D-Lysine (Sigma-Aldrich), according to manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: Virus medium mixed with fresh medium (1:1) was added to 10cm or 6-well plates with 10 µg/mL polybrene (TR-1003, Sigma Aldrich). 24 hours after infection the medium was discarded and changed to fresh medium ...
-
bioRxiv - Systems Biology 2023Quote: ... single cells were sorted based on their GFP signal into 384-well plates containing 100 nl of unique CEL-seq2 compatible primer (7 ng/µl) and 5 µl mineral oil (Sigma-Aldrich). Following cell lysis and denaturation ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell density was adjusted to OD600 = 0.4 and 4 μL of the bacterial suspensions were spotted on R2A agar plates either containing 0.5% sodium carboxymethyl cellulose (CMC; Sigma-Aldrich, C5678), 0.05% Remazol Brilliant Blue-Xylan (RBB-Xylan ...
-
bioRxiv - Systems Biology 2023Quote: ... 2*10^4 HEK293T cells in a well of a 96-well plate (coated with 0.05 mg/ml poly-D-lysin, Sigma Aldrich, P7405) were transiently transfected with 80 ng of a pcDNA3.1PA-D57 construct and 80 ng of a pFireV5-DM construct using PEI (1mg/ml ...
-
bioRxiv - Biochemistry 2023Quote: ... They were serial diluted and spotted to the YPD plates with and without addition of the indicated concentrations of myriocin (Sigma Aldrich). Plates were incubated for 2 days at 30 °C.
-
bioRxiv - Cancer Biology 2023Quote: Inserts and reservoirs in an IncuCyte ClearView migration plate (Essen BioScience, 4582) were coated with a solution consisting of 10 ug/ml fibronectin (Sigma, FC0105MG) to 1% BSA (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10 ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
-
bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
-
bioRxiv - Bioengineering 2023Quote: ... Radiochemical purity was assessed via thin layer chromatography (TLC) performed using aluminum TLC silica gel 60 F254 plates (Millipore Sigma, 105554) and a 75%:25% mixture of methanol and 1 M sodium acetate (pH 6.8 ...
-
bioRxiv - Cell Biology 2023Quote: ... 20’000 transduced MEFs were then seeded into one well of a 6 well plate in ‘MES medium’ supplemented with 2ug/ml Doxycycline (D9891-5G, Sigma-Aldrich), 3μM CHIR99021 (4423/50 ...
-
bioRxiv - Microbiology 2023Quote: ... A stock solution of 0.5 mM NPN in acetone was diluted to 40 μM in a clear flat bottom Corning 96 well plate (cat#CLS2585 from Millipore Sigma) with 5 mM HEPES buffer ...
-
bioRxiv - Microbiology 2023Quote: ... in the dark and all measurements took place in a clear flat bottom Corning 96 well plate (cat#CLS2585 from Millipore Sigma) with measured fluorescence at λex/λem 535/580 ± 20 nm on a Tecan Spark Plate Reader ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal volumes of cell suspension were plated onto the centre of the 96-well plates coated with poly-L-ornithine (P- 8638, Sigma-Aldrich). The cells were grown in DMEM/F12 medium (21331–020 ...
-
bioRxiv - Cell Biology 2023Quote: ... MPP4 (7 x 104 cells per well) were sorted directly into 8-well plates pre-coated with poly-lysine (Sigma-Aldrich) and containing XF DMEM medium ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 μL of the diluted cultures were transferred to a 96-well plate and sealed with a Breathe-Easy sealing membrane (Z380059, Sigma Aldrich). Growth at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... 4a-e, 4g, 4j, 5d-f) were cultured in 24-well plates (Falcon, 353047) on mixed cellulose ester Millicell inserts (Millipore, PIHA01250). Cells were plated at 100,000 cells/insert and cultured 6-8 weeks before experimental manipulations ...
-
bioRxiv - Immunology 2023Quote: ... These were subsequently plated in a 96-well V-bottom plate either in T cell media or T cell media containing PMA (50 ng/mL; Sigma-Aldrich), Ionomycin (500ng/mL ...
-
bioRxiv - Biochemistry 2023Quote: HEK 293FT cells were plated on six-well or ten-centimeter plates pre-coated with 0.1% (w/v) gelatin (Sigma-Aldrich # G9391). Once cells reached ~90% confluency ...
-
bioRxiv - Microbiology 2023Quote: The deep-well plate with the drugs and communities was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated anaerobically at 37 °C for 24 h ...
-
bioRxiv - Neuroscience 2023Quote: ... 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA, Sigma, cat. no A4544), 3 µM CHIR-99021 (Axon Medchem ...
-
bioRxiv - Microbiology 2023Quote: Cells were seeded the day before into 24-well plates containing sterile glass coverslips coated with poly-L-Lysine (Sigma-Aldrich). The seeding density per well was 150,000 cells for HEK293T and 50,000 cells for U2OS and Huh7 cells ...
-
bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
-
bioRxiv - Neuroscience 2023Quote: ... Astrocytes were cultured in Poly-L-Ornitine pre-coated flat bottom 12-well plate in a high glucose DMEM (Sigma, D7777) medium supplemented with 44 mM NaHCO3 (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... 104 iPSCs were seeded per well on ultra-low attachment V bottom 96-well plates in mTeSR medium supplemented with rock-inhibitor (Sigma-Aldrich). Twenty-four hours after seeding ...
-
bioRxiv - Cell Biology 2023Quote: ... 24-well glass-bottomed plates coated with Poly-L-ornithine/laminin (15 µg/mL Sigma Aldrich 10 µg/mL Sigma Aldrich), respectively ...
-
bioRxiv - Microbiology 2023Quote: ... 105 UFCs from an overnight culture were inoculated in 96-well plates (SPL Life Sciences) containing 200 µL of fresh MH (Oxoid) with doubling concentrations of trimethoprim (Sigma Aldrich). Plates were incubated at 37°C for 20 h without shaking ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was gently disaggregated and neurons plated for 90 minutes with DMEM 10% HS medium in P6 well plates previously treated with 1 mg/ml poly-L-lysine (Sigma Aldrich) at a density of 300,000 cells/cm2 ...
-
bioRxiv - Neuroscience 2023Quote: ... 48-well MEA plates (Axion, M768-tMEA-48B-5) were first coated with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich, P7886) and incubated in 37°C ...
-
bioRxiv - Neuroscience 2023Quote: Transfection of all cell lines (SH, NB7 and their Drosha k.o. clones) was performed in TPP 96 well plates (Millipore Sigma # 92696) in 250 μl final volume ...
-
bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were seeded on coated plates (Poly-L-ornithine [0.001%; A-004-C; Sigma-Aldrich] and laminin [300ng/mL; L2020; Sigma-Aldrich]) in 2i+LIF culture medium (N2B27 Media ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were suspended in plating medium either in 6-well plates coated with 0.05 M poly-D-lysine (Sigma, Cat# P7280) diluted in 0.05 M borate buffer (for protein extraction ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 µg/mL, Sigma-Aldrich, St. Louis, MO) and cultivated in DMEM medium containing 10% FCS and 2 mM L-glutamine at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: ... 1x106 cells per well were seeded in a 96-well round-bottom plate and stimulated with 100 μg/mL free OVA (Sigma-Aldrich) for 4 days ...
-
bioRxiv - Microbiology 2023Quote: ... cells were seeded on coverslips in 12-well plate and challenged with 100 pg/ml LPS (Sigma, L4391; diluted in PBS) for 12 hours ...
-
bioRxiv - Microbiology 2023Quote: ... 5 × 105 cells/mL were seeded in the wells of flat-bottom tissue culture plates and matured into macrophages with 20 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) during 72 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at 5×103 cells/50 µL/well of 96-well round-bottomed plates coated with Poly-2-hydroxyethyl methacrylate (12mg/ml; Sigma-Aldrich), and centrifuged at 200 x g for 5 min to form clusters ...
-
bioRxiv - Cell Biology 2023Quote: ... 24-well glass-bottomed plates coated with Poly-L-ornithine/laminin (15 µg/mL Sigma Aldrich 10 µg/mL Sigma Aldrich), respectively ...
-
bioRxiv - Developmental Biology 2023Quote: ... were cultured in Feeder-free on pre-coated plates with 0.5% growth factor-reduced Matrigel (CORNING 356231) (vol/vol in PBS with MgCl2/CaCl2, Sigma-Aldrich D8662) in E8 medium (made in-house according to39 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were seeded per well in 96-well plates with the indicated drug concentrations of entacapone and/or HLM006474 (SML1260, Sigma-Aldrich). After 72 hours cell viability was determined using a CellTiter-Fluor™ Cell Viability Assay (Promega ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Greiner Bio-One CELLSTAR Polystyrene 6-well Cell Culture Multiwell Plates coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS (Sigma-Aldrich), 1 h at 37℃ ...
-
bioRxiv - Bioengineering 2023Quote: We used 1x106 cells per sample for flow cytometry staining in a polypropylene 96-well round bottom plate (Sigma, cat#P6866). All staining steps were performed in 100 μL volume in the dark at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... substrates were placed in the 6-well plates and they were coated with poly-l-lysine (PLL, Sigma-Aldrich, MO, USA) solution prepared in ddH2O ...
-
bioRxiv - Bioengineering 2023Quote: ... For flow-NMR measurements 250 µL broth were sampled to a 96-deep well plate (DWP) and mixed with 500 µL Acetonitrile (Sigma Aldrich) by pipetting ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips placed in 24-well plates and treated with 10 μM of MG132 (M7449-200UL, Sigma Aldrich) diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
-
bioRxiv - Microbiology 2023Quote: ... and each transferred to its own well in a 96-well growth plate containing 1 mL of LB broth (Sigma-Aldrich) with 50 µg/mL kanamycin ...
-
bioRxiv - Microbiology 2023Quote: ... were measured to load approximately 5000 cpm for each sample on a silica gel 60 F254 thin layer chromatography (TLC) plate (Sigma-Aldrich). The TLC plate was eluted three times with petroleum ether/ethyl acetate 98/2 ...
-
bioRxiv - Neuroscience 2023Quote: ... the neurons were plated in 4-well plates coated with poly-L-lysine (1 mg/ml) (Cat. #: P2636-500MG, Sigma-Aldrich) and homemade laminin (Chauvet et al. ...