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Citations for Millipore Sigma :
3901 - 3950 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated for 1 – 1.5 h at 37°C followed by addition of warm DMEM with 5% fetal bovine serum and 2% carboxymethyl cellulose (CMC; Sigma) to achieve a 1% CMC overlay ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
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bioRxiv - Physiology 2023Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described (Crouch et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and exposed to blocking solution containing 2% bovine serum albumin or 5% normal goat serum (all from Sigma Aldrich, USA). The fixed cells were incubated overnight at 4 °C with the following primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were separated by chromatography by injecting 2 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, EMD Millipore). The flow rate was set to 150 μl/min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by chromatography by injecting 2 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, Millipore Sigma). Flow rate was set to 150 μl min ...
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bioRxiv - Cancer Biology 2024Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was injected into a ZIC-pHILIC 150 x 2.1 mm (5 um particle size) column (EMD Millipore). operated on Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... media was changed once per week with the addition of 1 μM 5-Fluoro-2′-deoxyuridine (Cat.#F0503, Sigma-Aldrich) and 1 μM Uridine (Cat.#U3003 ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... gilal cell growth and proliferation was inhibited by treating the cell cultures with 50μM 5-fluoro-2-deoxyuridine (Sigma-Aldrich). Mature cultures were used at DIV 11-12 for experiments and contained more than 95% neurons.
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was inj ected into a ZIC-pHILIC 150 × 2.1 mm (5 μm particle size) column (EMD Millipore). operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was dried by speedvac and reconstituted in 7.5 µL of 66% acetonitrile and 2 µL was separated by a ZIC□HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Day 25 differentiated kidney organoids were incubated with 10 µM of 5-Bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich, #B5002-100MG) in basal medium for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... washed three times in PBS/2%BSA for 5 min and then permeabilized using 0.1% Triton X-100 (Sigma-Aldrich) for 20 min followed by another washing cycle ...
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bioRxiv - Neuroscience 2023Quote: Cell proliferation was assessed at DIV2 by co-incubating cells with 10μM of 5-bromo-2’-deoxyuridine (BrdU) (#B5002, Sigma-Aldrich) in the last 4h of the pharmacological treatments ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mL of a 2.0×10^5 cells/mL solution were added to single cell culture dishes (EP0030700112, Sigma-Aldrich) and incubated overnight ...
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bioRxiv - Neuroscience 2023Quote: 5 dpf WT and scn1lab larvae were incubated in acridine orange (AO) (10 μM, Sigma Aldrich, CAS: 65-61-2) solution for 20 minutes followed by three 5 minute washes in embryo media ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Genetics 2022Quote: Fertilised eggs were identified by the presence of a sperm entry point and placed in wells cut into 2% noble agar lined 50 mm petri dishes containing 5% Ficoll 4000 (Sigma) in 1xMMR ...
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bioRxiv - Biophysics 2023Quote: ... The samples were blocked and simultaneously permeabilized using 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma Aldrich), in PBS (room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized in 0.3% PBS-Triton X-100 for 40 minutes and blocked in PBS/0.1% Triton X-100 containing 2% BSA and 5% donkey serum (#D9663, Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally three times daily for five consecutive days with 5-bromo-2’-deoxyuridine (BrdU, Sigma B9285-1G) diluted to 30 mg/kg in PBS ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Cell Biology 2024Quote: Propidium staining was conducted by 2 min incubation of 5-day-old Arabidopsis seedlings in propidium iodide (Sigma Aldrich P4864) diluted to 1 µg/mL in water ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
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bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
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bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
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bioRxiv - Cancer Biology 2021Quote: 5-Fluoro-5’-Deoxyuridine (Sigma Aldrich, F8791) and Oxaliplatin (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of HNO3 5% (Sigma, 84378) were added to each sample and they were placed 1h at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-PVA (5-phenylvaleric acid, Sigma) were dissolved in equimolar amount of NaOH to prepare a 0.5M stock solution with pH 7.
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bioRxiv - Microbiology 2021Quote: ... 5-aza-cytidine (5-AZAC, Sigma-Aldrich) and 5-deoxy-azacytidine (DAC-5 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5-fluorouacil (5-FU; Sigma-Aldrich) were dissolved in DMSO ...
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bioRxiv - Physiology 2024Quote: ... 5-hydroxytryptamine (5-HT, serotonin) (Sigma-Aldrich) and histamine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatants were incubated with 6 µl anti-HA (12CA5, in-house preparation) or 6 µl anti-T7 (69522, Millipore) and protein G-dynabeads (ThermoFisher Scientific ...