Labshake search
Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The non-adherent cardiomyocytes were seeded in complete Cardiomyocyte plating medium supplemented with 10% fetal calf serum and antibiotics at a density of 4-5 × 105 cells/well on Poly-L-Lysine (Sigma P-7890) and Gelatin (Sigma G9891)-coated coverslips in 6-well plates ...
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bioRxiv - Biophysics 2020Quote: ... 20 µL of 10 % Tween® 20 and 20 µL of a potassium phosphate buffer (4:5 mixture of 1 M monobasic and dibasic potassium phosphate, Sigma Aldrich, USA) were added as well as 10 µL of a 2 nmol thiol-modified single-stranded DNA solution (5’-T20-SH-3’ ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Bioengineering 2022Quote: ... The reaction was started by adding 30 µL of 100 mM (final concentration 5 mM) substrate 4-nitrophenyl β-D-glucopyranoside (Sigma-Aldrich). At regular five-minute intervals ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: Mice were treated topically on postnatal day 2.5 and 3.5 with 100 μl of an acetone solution containing 5 mg/ml 4-hydroxytamoxifen (4OHT) (Sigma, St Louis, MO, USA). Neonatal mice were irradiated on postnatal day 3.5 as previously described (Recio et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The cuticle’s pigmentation was bleached with 20% peroxide for 4-5 hours and the exoskeleton and tendons were stained with Congo Red (0.5 mg/mL, Sigma-Aldrich #C676-25G) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... were recovered from Matrigel using 3 mM EDTA in DPBS, washed, centrifuged (200g, 5 min, 4°C) and lysed with 1x RIPA buffer supplemented with PPI (1:100, Sigma- Aldrich, 04693132001), and benzonase (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Post-fixation and sequential decalcification were carried out at 4°C by immersing in the same fixative and with the addition of 5% EDTA (Sigma-Aldrich, US) for 5 hours each ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µl of the sample solution was mixed with 5 µl of a saturated solution of α-cyano-4-hydroxycinnamic acid matrix for MALDI-MS (Sigma-Aldrich) in acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... to an optical density at 600 nm (OD600) of 0.5 before 5-mL aliquots were treated with 180 uM 4-DPS (Sigma; Cat# 143057-1G) for 20 min at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were then rinsed 3 times with PBS and mounted onto microscope slides with 5 μl Mowiol 4-88 (Sigma-Aldrich #81381). Cells were visualized and imaged with a Nikon Ti-Eclipse A1M Confocal Microscope using a 60X objective ...
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bioRxiv - Physiology 2023Quote: ... Fixed cells were blocked and permeabilized overnight at 4°C in block solution (1x PBS + 5% bovine serum albumin (Sigma-Aldrich #A3803) + 0.5% Triton-X-100) ...
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bioRxiv - Developmental Biology 2024Quote: ... differentiation day 18 control and proximal-biased organoids were incubated at 37°C/5% CO2 for 4 hours in TeSR-E6 supplemented with 10 µg/mL TRITC-albumin (Sigma Aldrich, A2289). Control organoids were incubated with TeSR-E6 alone ...
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bioRxiv - Neuroscience 2024Quote: ... 4-5 non-embedded organoids at day 30 of organoid culture were pooled per sample and dissociated using Accuatse (Sigma, cat.no. A6964). Samples were first incubated with Accutase for one hour at 370C under dynamic conditions ...
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bioRxiv - Biochemistry 2024Quote: ... blotted and incubated overnight in 5% skim milk (GERBU Biotechnik, 70166) in TBST at 4°C with an anti-RAS antibody (Millipore, 05-516). As a loading control ...
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bioRxiv - Physiology 2019Quote: ... transgenic soybean hairy roots and tobacco leaves were used to determine GUS enzyme activity by measuring the fluorescence of 4-methylumbelliferone (4-MU) produced by GUS cleavage of 4-methylumbelliferyl-β-d-glucuronide (4-MUG, Sigma, USA) according to the published procedure as described previously (Jefferson ...
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bioRxiv - Molecular Biology 2021Quote: ... male WT (GR fl/fl, Cre/−) and GR knockout (GR fl/fl, Cre/+) mice (N=6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Plant Biology 2023Quote: CFDA: A 100 μl drop of 5(6)-carboxyfluorescein diacetate (CFDA, optimum excitation 490 nm, emission 515 nm) (C8166, Sigma, stock solution 50 mg/ml in acetone) was applied to the cut edge of a single cotyledon at a concentration of 0.1 mg/ml in distilled water ...
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bioRxiv - Cell Biology 2020Quote: ... 100 nM 4-hydroxy Tamoxifen (4 OT, Sigma, H7904) for 7 days was added on budding large organoids.
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bioRxiv - Neuroscience 2022Quote: ... and fixed with 4% PFA / 4% sucrose (Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 4-Nitroquinoline 1-oxide (4-NQO) (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 mM ethylenediaminetraacetic acid (60-00-4, Sigma-Aldrich), 0.1 M tris-HCl (1185-53-1 ...
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bioRxiv - Neuroscience 2021Quote: ... 100-300 μM 4-aminopyridine (4-AP, Sigma-Aldrich) and 10-30 μM XE-991 dihydrochloride (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... α-cyano-4-hydroxycinnamate (4-CIN, 250 µM, Sigma-Aldrich); iodoacetic acid (IAA ...
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bioRxiv - Neuroscience 2020Quote: ... then 4 % PFA (paraformaldehyde, Sigma Aldrich, 30525-89-4) in 0.1 M PB (phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 4-hydroxytamoxifen (4-HT; Sigma # H7904, St. Louis, MO) was injected into 2-3 months old PDGFRα-CreERTM;ROSA26-iDTa mice ...
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bioRxiv - Developmental Biology 2022Quote: ... 4-Hydroxytamoxifen (4-OHT, Sigma-Aldrich, cat. no. H7904) was added on day 5 at a concentration of 0.3 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 4-hydroxytamoxifen (4-OHT; H6278; Millipore Sigma, MA, USA) was made as described previously [19] ...
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bioRxiv - Cancer Biology 2022Quote: ... Treatment with 1.5μl 4 hydroxy tamoxifen (4-HT) (Sigma) (8 mg/ml) ...
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bioRxiv - Immunology 2023Quote: ... and 50 nM 4-hydroxy-tamoxifen (4-OHT) (Sigma) and re-fed at day 3 ...
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bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma Aldrich) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma-Aldrich #N8141) was added to a concentration of 1 μg/mL or 5 μg/mL ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were then incubated overnight at 4°C with rabbit polyclonal to elafin in 2% BSA at 1:100 dilution (Sigma, HPA017737). The signal was detected using the rabbit horseradish peroxidase EnVision kit (DAKOCytomation ...
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bioRxiv - Neuroscience 2019Quote: ... were expressed in 3T3 cells and IPed using 1µg anti-Fyn (mAb, sc-434, SantaCruz) or 4 µg anti-MAP2 (HM-2, mAb, Sigma, Cat # M4403) and Protein G beads (CalBiochem) ...
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bioRxiv - Immunology 2021Quote: ... mice were anaesthetised using inhaled isoflurane (4% (v/v) isoflurane and 2 l O2/min) and 30 μl of LPS (250 μg/mL, Sigma-Aldrich) or vehicle PBS were administered intranasally ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Samco Trident Vial Tall with PP Screw Cap) were incubated for 4 hours with 2 % aqueous Tween solution (Tween20, Sigma-Aldrich), then rinsed with deion ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Biophysics 2020Quote: ... maintains a pluripotent state of the stem cells.2–4 Cells were cultured in Glasgow modified Eagle’s medium (GMEM, Sigma Aldrich, Germany) supplied with 5.5 ml non-essential amino acids (Gibco Science ...