Labshake search
Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Loaded PDMS chambers were incubated for 30 minutes at 37°C and after polymerization muscle bundles were kept in normal proliferation medium containing 1.5 mg/ml 6-aminocaproic acid (6-ACA) (Sigma). After 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... Adult zebrafish (6 female and 6 male) were anesthetized in 0.02% tricaine (E10521, Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich). Shallow lesions were made on the skin (10 on each side ...
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bioRxiv - Neuroscience 2022Quote: Partial bilateral neostriatal dopamine lesions were made by injecting 6-OHDA (6 μg/μL, Sigma-Aldrich, St. Louis, MO) dissolved in ascorbic saline (de-ionized water containing 0.9% NaCl and 0.1% ascorbic acid ...
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bioRxiv - Cell Biology 2022Quote: C57Bl/6 mice (6 weeks-old) were treated with an ip injection of 1500 mg TAA/kg (Sigma-Aldrich) to induce ALF ...
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bioRxiv - Physiology 2022Quote: ... 0.5M K4[Fe(CN)6] and 0.5M K3[Fe(CN)6] with 1 mg/ml X-Gal diluted in DMF (all Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... each mouse received a bilateral injection of 1.25 μl of 6-hydroxydopamine hydrochloride (6-OHDA, Sigma-Aldrich, 4μg/μl) or vehicle (0.9 % NaCl + Ascorbic Acid 0.02% ...
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bioRxiv - Immunology 2024Quote: ... BEAS-2B.MR1-GFP cells were incubated with 94 μM 6-formylpterin (6-FP, Schircks) or the equivalent volume of 0.01 M NaOH (Sigma) overnight at 37°C at 5e4/well in a 24-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA rats received 2.3 μL bilateral injections of 6-OHDA hydrobromide (6 μg combined with ascorbic acid dissolved in 2.3 μL sterile 0.9% NaCl, Sigma Aldrich) in the SNc in each cerebral hemisphere ...
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bioRxiv - Developmental Biology 2023Quote: Pups were injected once daily intraperitoneally with 6-hydroxydopamine hydrochloride (6-OHDA) 50ug/g of body weight (Sigma H4381) from post-natal day 0 to 5 ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Neuroscience 2020Quote: ... animals were given vehicle or DMF (150 mg/kg) in 0.1% methyl cellulose via oral gavage in a total volume of 1 mL (Sigma, St. Louis, MO). Brains were collected an bulk mRNA isolated as described below 5 h after administration.
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bioRxiv - Developmental Biology 2020Quote: ... 10% nipagen (10% p-hydroxy benzoic acid methyl ester in 95% ethanol) and 1% propionic acid (Sigma Life Science, St. Louis, Missouri) to each solution ...
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bioRxiv - Plant Biology 2020Quote: Three-week-old tomato plants were placed under either WL or WL+FR for five days and were sprayed daily with a 50 μM methyl jasmonate (MeJA, Sigma-Aldrich®) or mock solution (0.1 % EtOH ...
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bioRxiv - Immunology 2020Quote: ... The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G) in PBS ...
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bioRxiv - Physiology 2022Quote: ... a sharp glass pipette (tip inner diameter: 15–30 μm) filled with NMDA (N-methyl-D-aspartate, Sigma-Aldrich, M3262, 0.2 mM), C21 (200 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: Cellulose scaffolds (Miniminit Products, R10, Scarborough, Canada) were partially infiltrated with a poly(methyl methacrylate) (PMMA, 120KDa, Sigma-Aldrich, St. Louis, USA) solution in acetone (Sigma-Aldrich ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: To extract polar metabolites from mouse (BLA tissue samples, a sample extraction buffer consisting of methyl tert-butyl ether (MTBE, Sigma-Aldrich, 650560), methanol (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... Tumour cells were cultured in 30 µl droplets containing 4000 tumour cells resuspended in spheroid medium (60 % growth medium and 40 % low viscosity methyl cellulose medium (25cp viscosity; Sigma Life Science), to which 3.6 µl of PureCol Type I Collagen (3.1 mg/ml stock solution ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Physiology 2023Quote: ... eNOS-independent relaxation was tested in presence of the pharmacologic eNOS inhibitor N-nitro-L-arginine methyl ester (L-NAME, 300 μM; Sigma-Aldrich N5751) either using increasing concentrations of the NO-donor dimethylamine nonoate (DEA-NONOate ...
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bioRxiv - Biophysics 2023Quote: ... delipidation solutions for 1–2 days at 37°C in a shaker, tB-PEG (containing 70% tB, 27% (v/v) poly (ethylene glycol) methyl ether methacrylate average Mn500 (Sigma-Aldrich, 447943) and 3% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were intravitreally injected with 1.5 μl of 20 mM N-methyl-d-aspartic acid (NMDA) (Sigma-Aldrich, St. Louis, MO). The 1.0mg/mL commercially available morphine solution (M-005-1ML in methanol ...
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bioRxiv - Plant Biology 2023Quote: ... followed by a 30-min treatment with 70 µL MSTFA [N-methyl-N(trimethylsilyl)-trifluoroacetamide (69479, Sigma-Aldrich, St. Luis, MO, USA) at 37°C and centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... cholesterol was delivered by incubating cells for 1 h with 100 μM of water-soluble methyl-β-cyclodextrin–cholesterol complex (Sigma-Aldrich) in DMEM/F-12 ...
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bioRxiv - Bioengineering 2024Quote: Polymer sheets were obtained by radical polymerization of a solution of either MA (methyl acrylate) or EA (ethyl acrylate) (Sigma-Aldrich, UK), using 1 and 0.35 weight percent benzoin (98% pure ...
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bioRxiv - Genetics 2021Quote: The Extensor Digitorum Longus (EDL) muscle was injured by intramuscular injection of 50 µL of 5 µM cardiotoxin (CTX) (Sigma, 11061-96-4). Mice were treated with carprofen 20 minutes prior to CTX injection and were injected with CTX under anesthesia by isoflurane ...
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bioRxiv - Plant Biology 2019Quote: ... raised against gel-purified ATLP1 using alkaline phosphatase(AP)-conjugated goat anti-rabbit IgG (1:10000 dilution) as the secondary antibody and 5-bromo,4-chloro,3-indolylphosphate(BCIP)/nitroblue tetrazolium (NBT)(Sigma Chemical Co. MS) as the substrates (Safadi et al. ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Sigma Aldrich, Taufkirchen, Germany) for 5-30 min.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Immunology 2020Quote: ... the Jurkat cells were transduced with TCR lentivirus at an estimated multiplicity of infection (MOI) of 5 with 1 μl of polybrene at a final concentration of 4 μg/ml (Sigma, St. Louis, MO). Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were infected with the corresponding lentiviruses (1/5 – lentivirus/cell culture medium) in the presence of 4 μg/mL polybrene (Sigma-Aldrich, cat# 107689). 4 days post-infection ...
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bioRxiv - Immunology 2024Quote: ... The biopsy had 4 mL of 37°C-warmed digestion buffer added (DPBS+/+ + 40 mL 5 mg/mL Liberase (Sigma Aldrich; SKU 5401127001) + 4 mL 1 mg/mL DNAse I (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Infective-stage promastigotes (metacyclics) were isolated from 4–5-day old stationary culture by density gradient separation by Ficoll (Sigma-Aldrich, Cat #F9378). Mice were infected with 105 L ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 μM DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) and 4 μM TAT-cre (Millipore, cat. no. SCR508) overnight.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from groups of 4-5 aphids of the above-described strains using TRI Reagent® (T9424, Sigma-Aldrich, USA) and Direct-Zol RNA extraction Kit (R2052 ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were perfused first for 5 min with NaCl 0.9% and then for 15 min with 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO) in sodium phosphate buffer (PB 0.1 M ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The colorimetric signal was subsequently detected using nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma-Aldrich, MO, USA) at 37 °C for a duration of 10 minutes.
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bioRxiv - Immunology 2024Quote: ... single cell suspensions were incubated for 4 hours at 37°C and 5% CO2 in supplemented RPMI 1640 media supplemented with PMA (50ng/mL, Sigma Aldrich, P8139-1MG), ionomycin (750ng/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Ireland) for 5 min at 4 °C using a Centricon 50 kDa molecular weight cut off (MWCO) concentrator (Millipore: MA, USA, Lot R0KA67379). After the 5 min spin ...
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bioRxiv - Plant Biology 2024Quote: ... medium lacking Trp but containing 40 μg mL-1 5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside (X-α-gal; Sigma‒Aldrich) at 30°C for 3 days ...
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bioRxiv - Microbiology 2024Quote: ... Membrane protein topology was assayed by plating the resulting reporter strains on dual-indicator plates containing LB agar supplemented with 80 μg/ml 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) ([Sigma Aldrich], RES1364C-A101X) and 100 μg/mL 6-chloro-3-indolyl-β-d-galactoside (Red-Gal ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Treatment with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich cat #H7904) and Shield-1 (Cheminpharma cat #CIP-S1-0005 ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-HOT) (H-7904, Sigma, St. Louis, MO), the active metabolite of tamoxifen ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μM 4–hydroxytamoxifen (4–OHT) (H7904–5MG, Sigma) for 3 hr to induce mCherry–FokI expression and 1 mg/ml doxycycline hyclate (D9891–G ...
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bioRxiv - Synthetic Biology 2020Quote: ... (Z)-4- hydroxytamoxifen (4-HT, H7904, Sigma-Aldrich, 10 mM), 3,3’,5-triiodo-L-thyronine (T3 ...