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Citations for Millipore Sigma :
4001 - 4050 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... or 4% paraformaldehyde/4% sucrose/1% TritonX-100 (Sigma-Aldrich), as described in (62 ...
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bioRxiv - Neuroscience 2019Quote: ... 4-Methylumbelliferyl β-D-glucopyranoside (4-MU) GBA substrate (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... and 100 µM 4-Aminopyridine (4-AP, #275875, Sigma Aldrich). GluR inhibition was performed using 40 µM D(-)-2-amino-5-phosphonopentanoic acid (APV ...
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bioRxiv - Cell Biology 2022Quote: ... and 2.5 μM 4-hydroxytamoxifen (4-OHT) (Sigma, Cat#H7904) to shuttle C/EBPα into the cell nucleus.
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bioRxiv - Cancer Biology 2022Quote: 10 mg/ml of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) was dissolved in acetone with gentle heating ...
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bioRxiv - Neuroscience 2024Quote: ... 4-hydroxy-tamoxfen (4-OHT) was purchased from Sigma (H6728) and was dissolved in 100% DMSO and stored in the -20 until use ...
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bioRxiv - Neuroscience 2024Quote: Recombination was induced with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich), as previously reported [6,11] ...
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bioRxiv - Neuroscience 2023Quote: ... 4-methylumbelliferone (4-MU) standard (Sigma-Aldrich®; Cat# M1381) was included in 0 ...
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bioRxiv - Physiology 2023Quote: ... and 4-aminopyridine (4-AP) were obtained from Sigma-Aldrich. We used standard locust saline to prepare all pharmacological agents ...
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bioRxiv - Neuroscience 2023Quote: ... 10mg of 4-hydroxytamoxifen (4-OHT, Sigma Aldrich #H6278-10mg) was added with 500μl ethanol and shaken at room temperature until the powder was completely dissolved (20mg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... The 4-OHT (4-OHT; Sigma, MO, USA Cat# H6278) was dissolved in ethanol to create a 20mg/ml stock solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-Methylumbelliferyl sulfate (4-MUS) substrate was from Sigma-Aldrich and GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tamoxifen (4-hydroxytamoxifen, 4-OHT) was obtained from Sigma (#H6278) and was reconstituted in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 4% paraformaldehyde (4% PFA; Sigma-Aldrich, cat# P6148) in 1x PBS pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... truncatula seedlings were transferred to Fahräeus medium supplemented with water (mock treatment) or 1 μM of 6-BAP (6-benzylaminopurine; Sigma-Aldrich) and maintained under the same growth conditions ...
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bioRxiv - Plant Biology 2019Quote: ... Inflorescences of each GUS line were pre-treated with ice cold acetone for 1h at −20°C and washed two times for 5 minutes with 100 mM sodium phosphate buffer followed by one wash with sodium phosphate buffer containing 1 mM K3Fe(CN)6 and 1 mM K4Fe(CN)6 (both Sigma) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Genetics 2020Quote: ... Transduction was carried out by infecting 2x105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... Transduction was carried out by infecting 2×105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... Some of the groups were then treated with 100μM 6-aminonicotinamide (6-AN) or dehydroepiandrosterone (DHEA) (both from Sigma-Aldrich), two pentose phosphate pathway inhibitors18 ...
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bioRxiv - Cell Biology 2023Quote: ... the newly formed bundles were kept in standard 2D muscle progenitor proliferation medium containing 1.5 mg/mL 6-aminocaproic acid (6-ACA) (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: C57BL/6 ES and C57BL/6-tdTomato+ ES cell lines were cultured on feeder cells with 2il medium: DMEM (Millipore) supplemented with 15% FBS (Gibco) ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Systems Biology 2022Quote: ... the pellicles were first digested by shaking for 16 h at 4 °C in 15 ml of PBS buffer with 2% cellulase (Sigma Aldrich, C2730).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: Zn2+release from DksA was measured using the metal chelator 4-(2-pyridylazo)resorcinol (PAR) (Sigma-Aldrich, St. Louis, MO, United States) (59) ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Biochemistry 2022Quote: ... sodium hydroxide (1310-73-2) and α-amylase (DFP Treated, Type I-A, saline suspension, 647-015-00-4) were purchased from Sigma-Aldrich Company Ltd. ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Biophysics 2023Quote: ... with a voxel-size of 0.636 × 0.636 × 2 μm) are recorded once before and 4 h after actin-depolymerization using 10 μM cytochalasin D (Sigma-Aldrich, St. Louis) using brightfield and second-harmonic generation imaging microscopy ...
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bioRxiv - Genomics 2023Quote: ... 160 μl of 2x lysis buffer (2 % Triton x-100, 100 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% sodium deoxycholate (Sigma-Aldrich, 302-95-4), 10 mM sodium butyrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... An amount of 2-4 mg of whole cell extract as incubated with 25 μL ANTI-FLAG® M2 affinity gel (Millipore Sigma) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells exposed to glutamine starvation were also treated with a freshly made 2 mM methionine sulfoximine (MSX, Sigma Aldrich 15985-39-4). The TCA precipitated samples were then centrifuged at 4000 rpm for 5 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... counted and 2-4×106 cells were washed twice in ice cold 0.9% saline made with NaCl (Sigma Aldrich, 31434-500G-M) and ultrapure HPLC grade water (Alfa Aesar ...
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bioRxiv - Evolutionary Biology 2019Quote: Five skate embryos were cultured from stage 23–30 in 900 mL of salt water (Instant Ocean) with/without all-trans RA (Sigma; final concentration of 2 × 10−6 M). After the culture ...
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bioRxiv - Cancer Biology 2021Quote: ... at 30 °C The lysate solution was added 2.5 μL 800 mM tris(2-carboxyethyl)phosphine (ALDRICH®) and 5 μL 100 mM iodoacetamide (SIGMA®) to in PCT-Micro tube to dilute into a final concentration of 10 mM and 40 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml DOX (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce TCF7L1 re-expression (DOX (+)) ...