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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 6 % (w/v) SDS (Sigma-Aldrich, #8.22050), 0.3 % (w/v ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cancer Biology 2020Quote: ... Each spheroid was made by resuspending 8,000 cells in 15 μl media with 0.24% methyl cellulose (4,000 cpi, Sigma-Aldrich, St. Louis, MO) by the hanging drop method 34 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Immunology 2019Quote: ... Methyl-β-cyclodextrin (MBCD) and water-soluble cholesterol (Xu et al. 2010) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... LMPs were cultured in this cholesterol-free medium for 20 t0 24 hrs and then treated with 10mM methyl-β-cyclodextrin (MβCD, Sigma C4555) for 15min at 37°C to remove cholesterol from plasma membranes ...
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bioRxiv - Systems Biology 2020Quote: ... TBDMS (N-methyl-N-(t-butyldimethylsilyl) trifluoroacetamide + 1% t-butyl-dimethylchlorosilane) and MSTFA (N-methyl-N-(trimethylsilyl) trifluroacetamide) were also purchased from Sigma-Aldrich. Media components and other reagents were purchased from Himedia and Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Plant Biology 2021Quote: ... tomato leaflets from the 3rd leaf were detached and immediately dipped for 10 sec in a 50 μM or 100 μM methyl-jasmonate (MeJA; Sigma-Aldrich) or mock solution (0.1 % EtOH ...
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bioRxiv - Bioengineering 2021Quote: ... with the control and labeling diets containing regular L-methionine (Met) and methyl-13C-L-methionine (13C-Met, 99%, Sigma-Aldrich), respectively (i.e. ...
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bioRxiv - Plant Biology 2021Quote: ... were permethylated by two rounds of treatment with sodium hydroxide (15 minutes each) and methyl iodide (45 minutes each) in DMSO (Sigma, USA) (46) ...
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bioRxiv - Physiology 2022Quote: ... 70 animals were placed onto each 50 mm tight-lidded petri plate with modified NGM containing 40 mM paraquat (or methyl viologen dichloride, from Sigma-Aldrich), 51 μM FUdR ...
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bioRxiv - Physiology 2022Quote: ... young adult animals were transferred to 1 mM auxin plates supplemented with 20 mM paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich). Animals were scored as in lifespan assays but twice a day ...
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bioRxiv - Physiology 2021Quote: ... animals were transferred at a density of 70 worms per plate to 50 mm tight-lidded plates with modified NGM containing 40 mM paraquat (or methyl viologen dichloride, from Sigma-Aldrich), 51 μM FUdR ...
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bioRxiv - Immunology 2020Quote: ... cells were treated for 1 h with a range of concentrations of the DNA methylating agent methyl methanesulfonate (MMS) (Sigma-Aldrich), and subsequently cultured for an additional 48 h ...
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bioRxiv - Biochemistry 2019Quote: ... trimethylsilyl-N-methyl trifluoroacetamide (MSTFA) and N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) were obtained from CS-Chromatographie Service and pyridine from Sigma-Aldrich at >99.98% purity ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... reacted with a mixture of 50 μL N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide (MTBSTFA; Sigma-Aldrich Co. LLC., St Louis, MO, U.S.A.) and 50 μL acetonitrile at 60°C for an hour.
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bioRxiv - Biochemistry 2020Quote: ... Samples were collected at various timepoints and the reaction was terminated by addition of SDS-PAGE sample buffer containing 100 mM methyl methanethiosulfonate (MMTS) (Sigma Aldrich). The PEGylation reaction samples were analysed using non-reducing polyacrylamide gels ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione) was purchased from Sigma-Aldrich (St. Louis, MO). 8MM-IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of PVDF-TrFE (70/30) (400 kDa., PDI of 2.1, Solvay Solexis, Inc., Thorofare, NJ) in methyl ethyl ketone (MEK; Sigma-Aldrich, Inc.) was loaded into a syringe with an 18-gauge needle ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were randomized and treated with 25 mg/kg Crizotinib or the corresponding dose of vehicle (hydroxypropyl methyl cellulose, Sigma Aldrich) (oral gavage ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were derivatized for GC-MS analysis by adding 10 μL of pyridine and 50 μL of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Sigma-Aldrich) to the residue ...
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bioRxiv - Cell Biology 2024Quote: ... Inhibition of NO in cell system was achieved by treating the cells with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, (1 mM) (#N5751; Sigma Aldrich) which is an analog of arginine ...
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bioRxiv - Biochemistry 2023Quote: ... Poly(methyl methacrylate-co-methacrylic acid) (85:15 monomer ratio) (Cat. No. 376914) (hereafter, referred to as acrylic copolymer (ACP)) (Sigma, USA) was purchased ...
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bioRxiv - Microbiology 2024Quote: ... In-cell SHAPE modifications were made by adding fresh 500µl of 100mM 1-Methyl-7-nitroisatoic anhydride (1M7) (Sigma-Aldrich, 908401) in DMSO to 4.5ml pre-warmed culture media to the dish and incubated for 3min at 37C ...
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bioRxiv - Immunology 2024Quote: ... the trypsin was used as is and the pepsin one was labeled at the C-terminal end using Arginine methyl ester dihydrochloride (Sigma-Aldrich) using the method used in (36).
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bioRxiv - Bioengineering 2024Quote: ... as inhibitor), and poly(ethylene glycol) methyl ether methacrylate (MEMA-PEG, 950 g/mol, MEHQ as inhibitor) are purchased from Sigma Aldrich and purified by recrystallizing in acetone to remove inhibitors ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and the remaining un-labelled EdU sites were then saturated by click-it chemistry with an excess of non-fluorescent azide (Azido-methyl-phenyl-sulfide 95%, Sigma, #244546): the reaction was prepared and achieved according to the manufacturer’s recommendations except the standard fluorescent azide was replaced by the non-fluorescent one at a final concentration of 2mM ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Cancer Biology 2023Quote: ... Nω-Nitro-L-arginine (L-NNA) (Catalog # N5501) and Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) (Catalog # N5751) were both obtained from Millipore Sigma and solubilized in 1M HCL and dH2O ...
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bioRxiv - Microbiology 2023Quote: ... The normal group was intraperitoneally administered a dose of 8 mL/kg of 0.5% carboxy methyl cellulose (Sigma-Aldrich, MO, USA) as an excipient ...
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bioRxiv - Synthetic Biology 2024Quote: ... The strain was transformed with a plasmid containing a 3-methyl benzoate (3MB; m-Toluic Acid; Sigma Aldrich Catalog No. T36609) inducible recT recombinase and mutLE36K (pTE583 ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Immunology 2021Quote: ... The membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-PALS-5 diluted 1:1,000 and mouse anti-tubulin (Sigma, catalog number T9026) diluted 1:3000 in blocking buffer) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Bioengineering 2021Quote: ... The gel compartment was removed after 1 to 5 days of incubation (depending on fusion) by adding 4 w/v% sodium citrate (Sigma Aldrich Inc.) for 15 min as a lyase for alginate (Supplementary figure 5 ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Neuroscience 2022Quote: ... The aqueous phase was transferred to a tube containing 500 μl of isopropanol (Fisher, A416P-4) and 5 μg of glycogen (Sigma, G1767-1VL). Then samples were pelleted at 18,213 x g for 15 min ...
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bioRxiv - Immunology 2022Quote: ... and was further purified by centrifugation at 10,000 xg for 5 minutes at 4°C through an Amicon Ultra 0.5 ml 100 kDa cutoff spin filter (Millipore, Billerica, MA, USA). The NEU3 was analyzed for size and purity by PAGE on ...
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bioRxiv - Bioengineering 2021Quote: ... tissue was washed 2X in methanol 100% for 4hrs and bleached overnight at 4°C in 5% hydrogen peroxide (Sigma-Aldrich 216763) in methanol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml; Sigma-Aldrich, www.sigmaaldrich.com). We measured mass spectra using an Ultraflex III MALDI-TOF (Bruker Daltonics ...
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bioRxiv - Pathology 2019Quote: ... The membranes were then washed in PBS-T and incubated overnight at 4°C in PBS-T containing 5% milk powder and primary antibody: monoclonal anti-GFAP (Sigma-Aldrich, G3893) in dilution 1:400 or monoclonal anti-actin (MP Biomedicals ...