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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... they were washed again 2 times with the wash buffer and incubated with 5% v/v goat serum (Sigma Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Tamoxifen was administered to Rosa26-MEK1 × SKA-MCM and Rosa26-MEK1 controls via intraperitoneal injections at 2 months of age for 5 consecutive days (75 mg/kg) using pharmaceutical-grade tamoxifen (Sigma) dissolved in corn oil (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Neuroscience 2020Quote: ... Worms were transferred at the L4 larval stage to NGM plates containing 50 μM 5-fluoro-2’deoxyuridine (FUDR, Sigma). The day of the timed egg laying was considered day 0 for lifespan analysis.
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bioRxiv - Cell Biology 2021Quote: ... which were diluted in the fluorescence dilution buffer (PBS supplemented with 5% FBS and 2% bovine serum albumin) containing 0.1% saponin (Sigma-Aldrich). After extensive washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were separated by chromatography by injecting 2-10 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, EMD Millipore). Flow rate was set to 150 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... was performed by intraperitoneal (IP) injection of 0.5 ml bacterial suspension containing 2×107 CFU/ml and 5% (wt/vol) mucin (Sigma-Aldrich). During experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduction was performed at a multiplicity of infection (MOI) of 2 in the presence of 5 µg/ml polybrene (Sigma). Cells were selected with 0.5-2.5 and µg/ml puromycin (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate, Sigma). After the desired color had developed ...
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bioRxiv - Microbiology 2020Quote: A549 cells infected with arboviruses or ACE2-SK-N-SH cells infected with SARS-CoV-2 (MOI=0.05-5) were treated with the RIPK2 inhibitor GSK583 (28) (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... but the slides had to be deparaffinized first with the following serial incubations: 2×5 minutes xylenes (Sigma, cat# 534056) twice ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Cancer Biology 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... The matrix consisted of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB, Sigma-Aldrich) at a final concentration of 10 mg/ml dissolved in chloroform ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... reduced with 1 mM tris(2-carboxyethyl)phosphine (TCEP), and alkylated by 5 mM iodoacetamide (30 min, room temperature, in the dark) (Sigma) and then quenched by 10 mM DTT ...
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bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained at 37°C in 5% CO2 atmosphere and treated with 2 µg/ml tunicamycin (Sigma-Aldrich), 10 nM-1µM thapsigargin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: Animals were injected intraperitoneally with 50 mg/kg BrdU (5-Bromo-2’-Deoxyuridine) (B5002, Sigma-Aldrich, St. Louis, MO, USA) for five days starting from PND 72 ...
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bioRxiv - Microbiology 2019Quote: ... PfCERLI1HAGlmS parasites were transfected with the an episomal cytosolic GFP expressing plasmid pHGBrHrBl-1/2 and maintenance of this plasmid was selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). Maintenance of the SLI-TGD plasmid was selected for using 20 μM WR99210 ...
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bioRxiv - Cell Biology 2019Quote: Cell proliferation was determined using the 5-ethynyl-2’-deoxyuridine (EdU)-Click 488 kit (BCK-EDU488, Sigma-Aldrich Co. LLC). Larvae were washed and resuspended in FASW to a density of ~500 larvae per ml ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed in dilutions of SSC (2x and 0.2x) and TNT prior to blocking (1-2 hours in blocking solution of 5% heat-inactivated horse serum (Millipore Sigma), 0.5% Western Blocking Regent (Millipore Sigma) ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... 1.7 M sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1x protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]). The sample was centrifuged at 16,000 g at 4°C for 45 min ...
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bioRxiv - Biochemistry 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Cell Biology 2021Quote: ... were resuspended in DPBS at 2 - 5 million cells per ml and incubated with 1 µl (25 - 29 U) benzonase (Millipore) at 37 °C for a minimum of 1 hr ...
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bioRxiv - Physiology 2020Quote: Excised hearts were placed in 1 μM Di-4-ANBDQPQ in Evans for 5 minutes and were placed in a dish containing 2% solution low melting point agarose (Sigma) dissolved in Evans solution ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Confluent ESCs monolayers were decidualized in DMEM/F12 containing 2 % FBS supplemented with 0.3 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma-Aldrich, USA), 10 nM β-Estradiol (E2 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were first incubated in 60% isopropanol for 5 minutes followed by staining with 2% Oil Red O (Cat# 00625, Sigma) in 60% isopropanol for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... samples were blocked and permeabilized with 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma-Aldrich now Merck) in PBS for 30 minutes at RT ...
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bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Biochemistry 2022Quote: ... The cysteine bonds were reduced with a final concentration of 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma, 646547) for 60 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Around 3 g of cells were suspended 1:5 in 50 mM Mops/KOH pH 7.0 supplemented with 2 mM dithiothreitol (DTT, Sigma-Aldrich) and disrupted by sonication while in an ice bath using a SONOPULS GM200 (Bandelin ...
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bioRxiv - Biophysics 2022Quote: ... 10 μM of biotin-labeled tubulin and 3.5 μM of Alexa647-labelled microtubules tubulin were incubated with 2 mM of GTP and 12 mg/ml CPA (Sigma) for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...