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Citations for Millipore Sigma :
4051 - 4100 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one was henceforth treated with 2 mg/ml DOX (Beladox, Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo re-expression (DOX (+)) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Genomics 2021Quote: ... PTA was carried out by first further lysing the cells after freeze thawing by adding 2 μL a prechilled solution of a 1:1 mixture of 5% Triton X-100 (Sigma-Aldrich) and 20 mg/ml Proteinase K (Promega) ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed four times in 1X PBS for 5 minutes and blocked with 2% Normal Donkey Serum (Millipore S30-100ml) before incubation with secondary antibodies in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 1X penicillin/streptomycin and anti-mitotic reagents (20 μM 5-fluoro-2-deoxyuridine and 20 µM uridine, both from Sigma-Aldrich). The medium replacement removed the electroporation buffer and dead cells.
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... plated in 96-well at a concentration of 2×105 cells/well and cultured for 72 h with medium alone or with OVA V (5 μg/ml; Sigma-Aldrich). Their supernatants were analyzed for cytokine expression using the V-Plex proinflammatory panel 1 mouse kit (MesoScaleDiscovery ...
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bioRxiv - Plant Biology 2019Quote: ... Matrix-assisted laser-desorption ionization time of flight mass spectrometry of the XyG oligosaccharides was recorded with a MALDI/TOF Bruker Reflex III using super-DHB (9:1 mixture of 2,5-dihydroxy-benzoic acid and 2-hydroxy-5- methoxy-benzoic acid; Sigma-Aldrich, sigmaaldrich.com) as matrix.
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... This was sonicated in a water bath at room temperature for 15 min before addition of 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and 50 mM chloroacetamide (Sigma-Aldrich) and incubated at 37 °C while shaking ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... For induction of the RRM2 knockdown 2 mg/ml Dox (BelaDox, Bela-pharm, Germany) was added in drinking water containing 5% sucrose (Sigma-Aldrich) for ad libitum uptake (Dox (+)) ...
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bioRxiv - Cell Biology 2021Quote: L4 stage or young adult worms were mounted on 2% agarose pads with 10 mM levamisole (CAS 16595-80-5; Sigma-Aldrich). A Zeiss LSM i880 microscope with Airyscan equipped with 40× Fluar objective (N.A ...
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were labelled with a PBS-Hoescht solution at 2 μg/mL for 5 min at RT and samples were finally mounted in mounting medium (F4680, Sigma Aldrich). Labelled cells and EVs were analysed with an inverted LSM 880 confocal microscope with airyscan (Carl Zeiss Ltd.) ...
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bioRxiv - Microbiology 2021Quote: HAE mucus was allowed to accumulate for one week prior to the application of 5 μl of 2 μm red-fluorescent (Cy3) polystyrene microspheres (1:50 dilution, Sigma-Aldrich) to the apical chamber of the transwell ...
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bioRxiv - Neuroscience 2020Quote: ... To inhibit the proliferation of non-neuronal cells a mitotic inhibitor 5-fluoro-2’-deoxyuridine (FDU, final concentration 10 μM, Sigma-Aldrich) was added with the change of the medium.
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bioRxiv - Animal Behavior and Cognition 2021Quote: At the end of the treatment sessions mice were subjected to intraperitoneal injections of BrdU (5-bromo-2-deoxyuridine, Sigma-Aldrich) at 50 mg/kg once a day during 4 days to label adult-generated progenitor cells in the DG of the hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... and used to spinfect H1299-H2AZ at 1000 rcf for 2 hours at room temperature in the presence of 5 μg/mL polybrene (Sigma-Aldrich). ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2020Quote: ... were loaded with 30 – 50 µg protein in 5X SDS loading buffer (250 mM Tris-HCl, pH 6.8, 10% SDS, 30% glycerol, 0.02% bromophenol blue [Sigma-Aldrich, B8026], 5% 2-mercaptoethanol [Sigma-Aldrich, M6250]) after heating the samples for 5 min at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... at a density of 2×104cells/cm2 in RHB-A medium supplemented with 5 ng/ml murine bFGF (Sigma, SRP4038-50UG). Medium was changed again every 2 days for the next 3 weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with 15 μL Fluoromount (Sigma F4680) for image acquisition ...
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bioRxiv - Pathology 2022Quote: ... The cultured C3H/10T1/2 cells were infected with 5 ml of retroviral supernatant for 48 h with 10 µg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... For immunohistochemical analysis of bronchiolar epithelium paraffin sections were first blocked with 5% goat serum and 2% fish gelatin (Both from Sigma-Aldrich) at 25 0C for 45 mins ...
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bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
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bioRxiv - Neuroscience 2021Quote: ... coupled to an electrochemical detector (Decade Lite-Antec Scientific) with a 5-μm Spherisorb ODS-2 reversed-phase column (Sigma-Aldrich) and the results were expressed as norepinephrine concentration in ng/g of tissue.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2021Quote: ... For blocking non-specific sites cells were incubated 30 minutes in PBG (5% BSA, 2% gelatin from cold water fish skin, from Sigma-Aldrich) and then stained for 2 till 16 hours with rabbit anti-IFITM3 polyclonal antibody (1:200 dilution from proteintech catalogue number 11714-1-AP ...
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bioRxiv - Immunology 2021Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/ml-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich) to the cell surface ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Hepatocytes were infected with HBV virus GTC (PXB Strain PBB004, Lot; 20151109) at 5 genome equivalents per cell in DMEM inoculum medium containing 2% DMSO (Sigma-Aldrich), 4% PEG-8000 (Promega) ...
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bioRxiv - Immunology 2022Quote: ... containing 100 nl water containing 7.5 ng/μl unique Cellseq-2 primers (Table S6) and 5 μl miniral oil (Sigma Aldrich, USA). Plates were stored at -80 °C until further use ...
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bioRxiv - Neuroscience 2019Quote: ... Dissociated DRG neurons were plated onto plastic coverslips in 24-well plates after washing and grown in culture medium (MEM in 5% fetal bovine serum, 20 μM 5-fluoro-2-deoxyuridine, 20 μM uridine, 100 units /ml penicillin and 100 ng/ml streptomycin, Sigma Aldrich) for 3 days at 37°C in a CO2-humidified incubator ...
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bioRxiv - Bioengineering 2020Quote: ... cells were centrifuged at 300 g for 5 minutes and resuspended in Essential 8 Flex medium containing 2 µM ROCK inhibitor Thiazovivin (Millipore Sigma). hPSCs were then counted using a Moxi Cell Counter (Orflo Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were randomized in two groups of which one henceforth was treated with 2 mg/ml BelaDox (Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo knockdown (Dox (+)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 15 minutes at −20°C and washed again with PBS after which aspecific antibody-binding was blocked for 2 hours in 5% bovine serum albumin (BSA, Sigma-Aldrich). Organoids were incubated overnight at 4°C with the primary antibodies (mouse anti-acetylated α-tubulin (Santa Cruz ...
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Microbiology 2019Quote: The extracted lipids were concentrated to dryness under nitrogen stream and then methylated by the presence of methanol and sulphuric acid (5% v/v) for 2 h at 85°C in the presence of 50 μg heptanoic acid (Sigma Aldrich) as internal standard ...
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bioRxiv - Physiology 2021Quote: ... 100 ng/ml basic fibroblast growth factor (FGF-2, ORF Genetis, Kopavogur, Iceland), 100 ng/ml epidermal growth factor (EGF, ORF Genetis) and 5 μg/ml heparin (Sigma-Aldrich). After 1 day in culture (5 % CO2/5 % O2) ...
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bioRxiv - Physiology 2021Quote: ... The samples were then washed twice for 5 minutes with the wash buffer containing 2% FBS (Sigma-Aldrich, cat. no. F7524) in PBS.
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bioRxiv - Plant Biology 2021Quote: ... 10 mM MgCl2, 250 mM sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1X protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]), and carefully overlaid on top of 500 μL of nuclear resuspension buffer 3 (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... 10 mM MgCl2, 1.7 M sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1x protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]). The sample was centrifuged at 16,000 g at 4°C for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin conjugates were eluted at 65 °C for 15 min in 2x Laemmli buffer containing 200 mM imidazole and 5% 2-mercaptoetanol (Sigma, M6250), vortexed for 90 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were equilibrated at 37°C for 5 min and subsequently initiated by addition of NADPH (Sigma, final concentration 2 mM). The reaction was allowed to proceed at 37°C and timepoints at 0 ...
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bioRxiv - Genomics 2021Quote: ... after which it was quenched with 5 mM of ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, Sigma, E3889). The quenched reaction was subsequently centrifuged for 5 minutes at 300 rcf and the supernatant was carefully discarded ...