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Citations for Millipore Sigma :
3851 - 3900 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with PBS-T at 4°C and the specific proteins were visualized with a chemiluminescence HRP substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Microbiology 2020Quote: ... aureus USA300 JE2 WT grown to OD1 and ΔesxC were washed three times before being treated with Proteomics grade trypsin from porcine pancreas (Sigma-Aldrich, UK) for 15 min as described (Solis et al. ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2021Quote: ... Quenched reactions were then removed from the glovebox and washed with buffer three times in a 10 kDa mini-concentrator (Millipore-Sigma, Burlington Massachusetts) and 5 uL of each sample was aliquoted into a screwcap vial (Waters ...
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bioRxiv - Biophysics 2021Quote: ... The samples were washed with PBS three times and then blocked in PBS with 5% (w/v) bovine serum albumin (BSA, Sigma-Aldrich A3059) overnight at 4°C.
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bioRxiv - Neuroscience 2021Quote: ... The cells were washed five times in PBS before mounting in polyvinyl alcohol (PVA) mounting medium with the anti-fading DABCO reagent (Sigma-Aldrich, Switzerland). The cells plated on CS were then examined with a microscope (EVOS ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction mixture was desalted three times and concentrated by ultrafiltration (Ultracel 100 kDa Ultrafiltration disc with 100 mM phosphate buffer, pH 6.9, Merck Millipore, Burlington, MA, USA) to a final volume of 12 ml.
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times and HRP-conjugated Abs (goat anti-mice IgG and IgM) (Sigma-Aldrich, St. Louis, MO, USA) were added at 1:1500 dilution in 0.5% HSA/PBST (100 µL/well ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the percentage of infected RBCs) was assessed at each time point by microscopic counts of thin blood smears stained with Giemsa solution (Sigma -Aldrich, USA).
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three more times in 1x PBS for 10 minutes and coverslipped using a Mowiol solution (10% Mowiol - Sigma, 25% glycerol). Slides were allowed to dry in the dark at room temperature and subsequently imaged using fluorescent microscopy ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were then washed 3 times in 0.5% PBSTx for 5 minutes per time and incubated in Hoechst 33258 (use: 1 in 1000; stock: 20mg/ml; Sigma-Aldrich; B2883) for 30-60 minutes.
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bioRxiv - Bioengineering 2022Quote: ... cells were grown to 100% confluency and then fed 3 times a week with adipogenic medium (growth medium with 10 μg/mL insulin (Sigma Aldrich #I0516), 0.5 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized in ice-cold methanol by dipping the coverslip in it 20 times followed by blocking in 3% BSA (Sigma-Aldrich; A9647) in PBS for 15 min (Figures 2 A-H and S1) ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 times with D-PBSX before incubation with the corresponding secondary antibodies (all diluted 1:500) and DAPI (Sigma, #32670-25mg) for 1 h (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... Then the samples were washed 3 times with PBS and then incubated for 1 hour with the donkey α mouse Cy3 (1:200; Millipore Chemicon), donkey α rabbit Cy5 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Cortex were microdissected and homogenized on ice with polypropylene pellet pestles in three times the brain tissue weight in RIPA lysis buffer (Millipore 20-188) containing PhosSTOP (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... and absolute for 1 min each and washed three times with 1× DEPC-PBST (1x DEPC supplemented with 0.05% Tween-20, Cat No. P9416; Sigma-Aldrich, MO, USA). Permeabilization of tissue was carried out in 0.1 M HCl for 5 min and then washed three times with 1× DEPC-PBST.
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore, Billerica, Massachusetts, USA) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore, Billerica, Massachusetts, USA). After filtration through a 0.22 µm filter (Whatman ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting conjugated antibody was purified by washing for five times with an Amicon Ultra filter with 50 kDa NMWL (Millipore Sigma, UFC505096). The conjugated antibody was quantified in IgG mode at A280 using a NanoDrop (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed five times for 10 minutes each in TBS-T and detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... or 50 µM indolmycin (BioAustralis Fine Chemicals) for ∼ 50 times the delayed death IC₅₀) and supplemented with 5 µM GGOH (Sigma Aldrich, G3278) to induce delayed death while rescuing the prenylation defect ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed several times in PBSDT and endogenous peroxidase activity was inactivated by treatment with 6%H2O2 (Sigma-Aldrich, Cat# H1009) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the coverslips were washed three times in PBS and incubated with donkey anti-rabbit CF 647 (Sigma, SAB4600177, 1:2000 dilution) and goat anti-rat AlexaFluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... They were then washed three more times with PBS and blocked for 1 hour at room temperature using 10% BSA (Sigma, Cat# A7906) in 1X PBST (PBS + 0.2% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated parasites were washed three times with ice-cold PBS containing a protease inhibitor cocktail (Roche cOmplete mini, Sigma Cat. No. 11836153001) before lysis in 1x Laemmli Sample Buffer (Biorad Cat ...
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bioRxiv - Microbiology 2024Quote: ... samples were washed three times with PBS and cell nuclei were stained with DAPI (1 μg/ml in PBS, Sigma-Aldrich D9542) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... plates were again washed three times with PBST prior to the addition of 100 μL of o-phenylenediamine dihydrochloride (Sigma-Aldrich; OPD). Plates were allowed to develop for 10 minutes after which the reaction was stopped via the addition of 50 μL of 3M hydrochloric acid (HCl) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Bioengineering 2024Quote: ... embryos were washed five times in PTW and finally transferred into optical clearing solution46 (20% (wt/vol) urea (Sigma Aldrich, Cat#: V900119), 30% (wt/vol ...
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bioRxiv - Neuroscience 2024Quote: ... The GFP selector beads along with bound proteins were recovered by a brief centrifugation and washed two times with LB containing 0.1% SDS before incubating with 1 M hydroxylamine hydrochloride (HAM, Sigma, St. Louis, MO) in LB (pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Samples for RNA extraction were washed in M9/0.1% Tween-20 in 15 mL conical tubes at least three times before adding 1 mL of TRI-Reagent® (Sigma-Aldrich). Total RNA extraction and ethanol precipitation protocols was performed as per Tri-Reagent® manufacturers instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed two times for 5 min with PBS 1X and coverslip mounted with Fluoroshield™ with DAPI (Sigma, F6057). Z-stack images were obtained at a 60-x magnification using an FV1000 Fluoview Confocal Laser Scanning Biological Microscope (Olympus® ...
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bioRxiv - Immunology 2022Quote: ... ELISPOT plates were washed three times with sterile 1XPBS and blocked for two hours at 37°C using RPMI 1640 (Sigma, Cat. #R0883) supplemented with 1% L-glutamine (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... 70 and two times in 100%) for 12 h each at room temperature and cleared with ethyl cinnamate (Sigma-Aldrich, Taufkirchen, Germany) at room temperature for 24 h according to the simpleCLEAR protocol15 (Fig ...
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bioRxiv - Bioengineering 2023Quote: ... The surface was washed with sterile Milli-Q® water three times prior to a laminin solution (20 μg/ml, Sigma-Aldrich) being added and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: Wild-type and tg-Xist mice were injected with a one-time injection of 0.5 mL of pristane (Sigma-Aldrich, P9622-10X1ML) at 8-10 weeks old (SJL/J strain ...
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bioRxiv - Plant Biology 2023Quote: ... Sugars and were identified by comparison of retention time and mass spectra with authentic standards (Sigma, Sigma-Aldrich, St. Luis, MO, USA), while other metabolites were identified using NIST14 (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and pellet was washed once with PBS and another time with 1 ml ice-cold 155 mM ammoinium bicarbonate (AB, Sigma-Aldrich, FL40867) until finally resuspended in same buffer to 5000 cells/µl ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed three times with PBS/ 1% BSA and then incubated with 0.5 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for seven minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Each sample was obtained by flushing three to five times the vagina with saline solution (NaCl 0.9%, Sigma-Aldrich, St. Louis, MO). The final flush was placed on a glass slide ...
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bioRxiv - Biochemistry 2022Quote: ... 0.6 mL bed volume of resin was washed three times with GF1 buffer and blocked by incubation with 100 μM biotin (Sigma, cat. #B4501) in 50 mM HEPES/KOH pH 7.5 for 5 min on ice with occasional mixing ...
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bioRxiv - Cancer Biology 2023Quote: ... beads washed five times with pre-chilled NET resuspension buffer and eluted under native conditions by competition using 3x FLAG peptide (Millipore Sigma, F4799). Samples were boiled in the Laemmli SDS sample buffer (Thermofisher ...
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bioRxiv - Biophysics 2023Quote: ... We washed the membrane 3 times with TBST before incubating with a 1:1 ratio of horseradish peroxidase substrate and enzyme mixture (Millipore, Burlington, Massachusetts). We imaged using a Biorad Chemidoc camera set to detect chemiluminescence ...