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Citations for Millipore Sigma :
3951 - 4000 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... For tumor induction 8-week old mice and Cre negative Ptchf/f animals were orally administered three times with 1 mg TAM (Sigma, St Louis, MO) dissolved in 10% ethanol/corn oil ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 3 times over 45 mins before fixation in phosphate-buffered saline (PBS) containing 4 % paraformaldehyde and 4 % sucrose (PFA, Sigma-Aldrich, Cat.# P6148) at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Glycine was added to a final concentration of 0.125 M to quench the cross-linking reaction and washed three times with ice-cold PBS with cOmplete protease inhibitors (Millipore Sigma Cat# 11836170001). The cells were then prepared for sonication using the truChIP chromatin shearing kit (Covaris Cat# 520154) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 spike-pseudotyped viruses were then concentrated 10 times at 2000×g using 100 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore. Cat. No. UFC910024).
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bioRxiv - Cancer Biology 2020Quote: ... U373MG cells were seeded at a density of 1×104 (24 and 48hr exposure time points) in 96 well plates (Sigma-Aldrich, Arklow, Ireland) with 100µl media per well ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixing buffer was removed and cells were washed five times with 200 uL PBS containing 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) for permeabilization ...
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bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times with 1X PBS prior to blocking with 3% bovine serum albumin (BSA; Sigma #A7906, Saint Louis, MO) in 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... media was exchanged with pre-warmed DMEM without serum but supplemented with various inducers for different lengths of time (2 μM Ci A23187 (Sigma-Aldrich, C7522-1mg) for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... was washed with 5 L Milli-Q water for 4 times and then incubated with dialysis tubing (INTERCHIM, T4-25-30) filled with Dowex (Sigma-Aldrich, 14015-U) for 12 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The fatty acids were identified by comparison of the retention times of a standard solution of 37 fatty acid methyl esters (Sigma; 47885-U Supelco) in GC-FID and confirmed by high accuracy mass of molecular ions and their fragments after injection into a GC-MS (Q-Exactive™ ...
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bioRxiv - Cell Biology 2020Quote: Infected HepG2 or HeLa cells were fixed at the indicated time points in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS, 137 mM NaCl (Sigma-Aldrich, S9888-1Kg), 2.7 mM KCl (Fluka Chemie AG P9541-1kg) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue sections were incubated with primary antibodies at 4° C overnight and then washed three times with PBS containing 0.2% Triton X-100 (PBSTx; Sigma-Aldrich, St. Louis, MO). The following antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with TBST three times for 5 minutes and incubated with a 1:25,000 dilution of horseradish peroxidase-conjugated anti-mouse (Sigma, Cat no. # A9044-2ML6) or anti-rabbit (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... animals were boosted 5 times in a 3 week-interval with 0.2 mg human TNFa in incomplete adjuvant (Sigma Chemical, St. Louis, MO). Final boost was given intravenously with 0.4 mg the protein in PBS four days before splenectomy ...
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bioRxiv - Biochemistry 2021Quote: ... Identification of FAME was done by comparison of retention times and mass spectra with those of authentic reference chemicals (FAME: reference mixture of 37 FAME, Sigma-Aldrich, Deisenhofen, Germany). As labelled reference ...
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bioRxiv - Neuroscience 2020Quote: ... On the day of the experiment the cells were placed on ice and washed 3 times with PBS supplemented with 10mM EDTA (Sigma, 150-38-9) for 3 minutes each ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads were washed 3 times with lysis buffer and TFEB or TFE3 eluted using 200 ng/μL 3X-flag-peptide (Sigma-Aldrich, F4799-4MG).
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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Single cell kinetic modeling of redox-based drug metabolism in head and neck squamous cell carcinomabioRxiv - Systems Biology 2022Quote: ... cells were washed three times with PBS and siRNA packaged in lipid nanoparticles using the N-TER Nanoparticle siRNA Transfection System (Sigma-Aldrich, Cat#N2913) was applied to each well at 50 nM in 100 uL of serum-free media for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... we filtered 500 ml of the collected water through a 0.22 μm Sterivex filter (Millipore Sigma, Burlington, MA; n=4 per time point) using peristaltic pressure ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The sugar feeder was a yellow plastic plate surrounded with 4 filter papers containing a 5µl drop of 100 times diluted linalool racemic mixture (Sigma-Aldrich, St. Louis, Missouri). Then ...
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2021Quote: ... cells were diluted 1:1000 times into fresh MH medium supplemented with a surfactant (Pluronic F-108; 542342; Sigma-Aldrich; 0.085% (wt/vol) final concentration) ...
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bioRxiv - Immunology 2019Quote: ... the plates were further washed with PBST for three times and incubated with 50 µL 1000X diluted SA-HRP (Sigma-Aldrich, MO, USA). After 3 washed with PBST ...
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bioRxiv - Genomics 2021Quote: ... aliquoted in equal cell numbers to T-25 or T-100 ThermoFisher Tissue Culture Flasks (each flask corresponding to one time point) and treated with Triptolide (Sigma-Aldrich, T3652-1MG) or Trichostatin A (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained neurovascular units ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then washed three times with PBS (5 minutes each wash) and permeabilised for 15 min using 0.5% triton-X100/PBS (Sigma-Aldrich. Ref: T9284-500ml). Cells were blocked for 1 hour in 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed with 1X PBS three times (1×10 min, 2×5 min) and incubated for 10 min in 0.5% Thio-S (T1892; Sigma-Aldrich, St. Louis, MO) diluted in 50% ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Quenched reactions were then removed from the glovebox and washed with buffer three times in a 10 kDa mini-concentrator (Millipore-Sigma, Burlington Massachusetts) and 5 uL of each sample was aliquoted into a screwcap vial (Waters ...
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bioRxiv - Biochemistry 2021Quote: ... were transiently transfected with expression plasmid encoding HexaPro/Furin using FectoPro transfection reagent (Polyplus-transfection® SA, Cat. #116-010) with 5 mM sodium butyrate being added at the time of transfection (Sigma, Cat. # 303410). After 4-5 days post-transfection time cells culture was harvested ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Microbiology 2021Quote: ... were orally gavaged at the beginning of the light cycle (between Zeitgeber time 0-3) with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO) or 0.3 ...
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bioRxiv - Genomics 2021Quote: ... Cells were then washed 3 times with PBS and incubated with 0.05 mg/ml Filipin III from Streptomyces filipinensis (Sigma; Cat. No. F4767-1MG) in PBS for 2 h at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were subsequently washed 3 times with PBS for 10 minutes and mounted in Duolink in situ mounting medium with DAPI (Sigma-Aldrich, #DUO82020-5ML).
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed four times with 500 μL of Binding Buffer and then eluted with 2X Sample Buffer (Millipore Sigma #S3401-10VL). The input and bound fractions were then analyzed via Western blotting using standard conditions.
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were washed three times with PBS and lysed in RIPA buffer containing protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO). Total protein concentrations in the lysates were determined by bicinchoninic acid (BCA ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in citrate-PBS and washed 4 times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Sigma-Aldrich, Zwijndrecht, The Netherlands) to remove all Lymphoprep ...
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The organoids were washed three times for 15 minutes with 1x PBS (Roth, Cat. no. 1105.1) and then incubated in 30% sucrose (Sigma Aldrich, Cat. no. S7903) in PBS solution at 4°C until they sunk to the bottom of the dish ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Cell Biology 2023Quote: ... PDMS substrates were rinsed with sterile water three times and coated with 50 µg/mL of fibronectin (F4759, Sigma-Aldrich, St. Louis, MO) diluted in water for one hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike-pseudotyped viruses were then concentrated 10 times at 2000×g using 100 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore. Cat. No. UFC910024).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... dehydrated through a series of increasing concentrations of ethanol and cleared three times for 5 minutes in xylene (Sigma Aldrich, cat. no. 247642). At the end ...