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Citations for Millipore Sigma :
3651 - 3700 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the plates were washed with PBS four times and horseradish peroxidase-conjugated anti-mouse IgG or horseradish peroxidase-conjugated anti-mouse IgA (Sigma Aldrich) diluted 1 ...
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bioRxiv - Developmental Biology 2020Quote: Larval brains of various genotypes expressing EB1-GFP under grh-Gal4 at various time points were dissected in Shield and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue was washed 3 times with RPMI and then resuspended in a solution of 10 % Dispase (Cornig) 30 μg/ml DNAse I (Sigma-Aldrich) in RPMI (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: ... ovaries from 12-16 weeks old females were dissected and GV oocytes were released from the follicles by puncturing the ovaries several times with 27-gauge sewing needles in MEM/polyvinylpyrrolidone media containing 2.5 μM milrinone (Sigma-Aldrich; M4659) to prevent oocyte maturation after the release from cumulus cells ...
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bioRxiv - Neuroscience 2021Quote: ... Then the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich). Images were acquired using a Zeiss 780 inverted confocal microscope ...
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bioRxiv - Cell Biology 2020Quote: Fixed accessory glands were stained at room temperature in the following solutions and then washed four times in PBS: 1:50 dilution in PBS of a 10 mg/ml solution of Nile red (Sigma-Aldrich) dissolved in acetone and incubated for 30 min ...
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bioRxiv - Genomics 2021Quote: ... randomly assigned mice were gavaged at Zeitgeber time (ZT) 0 with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO), 0.01 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The specimens were washed in phosphate buffer three times before being post-fixed in 1% (w/v) osmium tetroxide (Sigma-Aldrich) at 25°C for an hour ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]) ...
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bioRxiv - Neuroscience 2021Quote: ... Unconjugated p-Cresol was identified by comparing with the accurate mass and the retention time of the reference standard (p-Cresol, Sigma-Aldrich) in our in-house library.
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bioRxiv - Neuroscience 2019Quote: ... Analytes were identified by matching criteria of retention time and sensor ratio measures to known standards (Sigma-Aldrich, St. Louis, MO). Compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Physiology 2021Quote: ... Samples were mixed 10 times with a 1 ml pipette before being filtered through a 100 μm and 30 μm strainers (Falcon, Sigma Aldrich) to remove large aggregates ...
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bioRxiv - Cell Biology 2020Quote: ... Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution; SIGMA; cat T8154).
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bioRxiv - Cell Biology 2021Quote: ... Ears were then washed for the last time (by vortexing in wash solution) and pinned on a silicone-based matrix (cat # 761028, Sylgard, Sigma-Aldrich) in a 6-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed three times with PBS and mounted with a specific medium added with nuclear staining (Fluoroshield DAPI mounting medium, from Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... The obtained single-cell suspensions were then washed three times with PBS supplemented with 0.04% BSA (Sigma, St Louis, MO, USA) and were filtered with a 40 μm cell strainer (BD Falcon ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... The resin was washed 5 times and transferred to a spin column with 40 μl of 3 X Flag peptide (Sigma, #F4799) for 1 h at 4°C on a rotator ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Compounds with VIP>1 and adjusted p-value<0.05 were tentatively annotated by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Larvae were washed with RPMI 1640 three times to remove ampicillin and separated from egg shells and unembryonated eggs using a stepped 50-60% Percoll (Sigma Aldrich) gradient ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... Preparations were washed one time in brain-Pblec buffer and incubated with isolectin B4 FITC conjugated (1:80, L2895; Sigma-Aldrich) in Pblec buffer at 4C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was homogenized 20 times with pestle A and 20 times with pestle B in 2 ml of ice-cold nuclei EZ lysis buffer (NUC101-1KT, Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2021Quote: ... Novec™ 7500 was removed by pipetting and residual oil was removed by washing two to three times with hexane (Sigma). Samples were then washed three times with PBS to remove dextran from the system ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed for ten minutes three times in PBT (0.2% Triton X, T8787; Sigma-Aldrich and PBS, P3813; Sigma-Aldrich) on an orbital rocker (SK-O180-S ...
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bioRxiv - Immunology 2022Quote: ... The coating solution was then flicked off and the plate was washed three times with PBS containing 0.1% Tween® 20 (Sigma Aldrich). Plates were then blocked with PBS containing 10% FCS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... this culture was incubated for a further 1 hr before washing three times and RNA was extracted using Triazole (Sigma Aldrich) following manufactures protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings were washed gently three times (10 min for each wash) in PBS and then the cell wall was digested in 2% Driselase (Sigma, D8037) in PBS for 18 min at 37 °C and washed five times with PBS ...
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bioRxiv - Plant Biology 2022Quote: ... MS Spectra were processed with the software “GCMS Solutions” supplied by Shimadzu to adjust retention times between each run using a mixture of alkanes (C7 to C40, Sigma-Aldrich). Peak identity was established according to their retention time and mass fragmentation spectra with respect to authentic standards derivatized (Table S1) ...
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bioRxiv - Neuroscience 2022Quote: ... The clarified media was then sterile filtered and concentrated by 30 times using Amicon Ultra-15 100 kDa Centrifugal Filter Units (EMD Millipore). For the pressure myography and the awake two-photon imaging experiments ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was finally washed again in PBS three times for 10 minutes before mounting using FluorSave mounting medium (Merck-Millipore #345789). For immunostainings against MBP ...
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bioRxiv - Biochemistry 2022Quote: ... Sample was then concentrated 6 times by ultrafiltration using a 3 kDa molecular weight cut off Centricon centrifugal filter units (EMD Millipore) and applied onto a Superdex 200 10/300 increase (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The cells were washed in PBS three times and then reduced for 10 minutes with 50 mM NH4Cl (Sigma Aldrich, 254134), followed by three additional washes in PBS.
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bioRxiv - Cell Biology 2022Quote: Larval brains of various genotypes expressing EB1-GFP under grh-Gal4 at various time points were dissected in Shield and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: Larval brains of various genotypes expressing UAS-mCD8-GFP under grh-Gal4 at various time points were dissected in Shield and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed at room temperature three times for 5 min each with 1x PBS (Sigma Aldrich, cat. no. 806552). Cells were then permeabilized with 1x PBS 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... live neurons were imaged as above and time-lapse movies were obtained continually with a 1 second interval before and after 100 μM Antimycin (A8674, Sigma-Aldrich) addition.
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bioRxiv - Immunology 2022Quote: ... Transport activity was terminated by rapid removal of the uptake medium and subsequent washing three times with cold PBS with 25 mM glucose (Sigma, G7528). Cells were lysed with 0.5 ml of 0.5 M NaOH (Fisher Scientific ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: Zebrafish larvae were collected at indicated time and soaked in Neutral Red (2.5 mg/mL, Sigma-Aldrich, St Louis, MO, USA) overnight at 28.5 °C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with phosphate buffered saline (PBS) and fixed in 4% (w/v) paraformaldehyde (Sigma-Aldrich, Mumbai, India). The fixed cells were air dried on slides and observed with 60% glycerol mountant ...
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bioRxiv - Microbiology 2022Quote: ... and at various times they were treated with 10 mM N-ethylmaleimide (NEM; Sigma-Aldrich, cat. No.-E3876, St Louis, MO) for 5 min at room temperature followed by fixation with 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2022Quote: ... were removed at each time point and quenched by adding 2 µL of 6X stop solution (25 mM EDTA (Sigma-Aldrich), 0.5 U/µL proteinase K (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The identification of amino acids was based on retention times and m/z values obtained from amino acid standards (Sigma-Aldrich)
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bioRxiv - Neuroscience 2023Quote: ... The analytes were identified by the matching criteria of retention time measures to known standards (Sigma Chemical Co., St. Louis MO). Compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Microbiology 2023Quote: ... The resulting mixture was passed multiple times through a 0.45-μm Millex®-HV pore-size filter (Millipore, Billerica, MA, USA) to remove remaining prey ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in cold DPBS three times and lysed directly in the cell culture wells with RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Sigma-Aldrich). Lysates were cleared via centrifugation at 20,000 rcf at 4 °C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5 % potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 2 times with PBS (10 min each) and finally mounted onto slides covered with DAPI mounting media (#F6057; Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The analytes were identified by the matching criteria of retention time measures to known standards (Sigma Chemical Co., St. Louis MO). Compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...