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Citations for Millipore Sigma :
4001 - 4050 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... resuspended in citrate-PBS and washed 4 times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Sigma-Aldrich, Zwijndrecht, The Netherlands) to remove all Lymphoprep ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained enriched neurovascular fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... the two were mixed gently at a ratio of 1:2 (v/v) and resuspended in 20 times ice-cold Collagen (Sigma, Cat # C0130-1G). Cells were then aliquoted in 20 µl volumes into a 48-well plate and allowed to polymerize at 37□°C for 45Lmin ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The organoids were washed three times for 15 minutes with 1x PBS (Roth, Cat. no. 1105.1) and then incubated in 30% sucrose (Sigma Aldrich, Cat. no. S7903) in PBS solution at 4°C until they sunk to the bottom of the dish ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were washed three times in 0.4% PBST before mounting on glass slides in mounting medium (Mowiol 4-88 anti-fade agent, EMD Millipore Corp., Billerica, MA). The following primary antibodies were used ...
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bioRxiv - Microbiology 2024Quote: ... this culture was incubated for a further 1 h before washing three times and RNA was extracted using Triazole (Sigma Aldrich, England, UK) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Glomerular DNA was used for PCR using the RedTaq Ready MasterMix (Sigma-Aldrich, St. Louis, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with RED extract-N-Amp Tissue PCR reagents (Millipore Sigma, Saint-Louis, MO) to purify genomic DNA and perform the genotyping PCR ...
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bioRxiv - Biophysics 2022Quote: ... sub-cloned into the pNKS2 vector) via PCR with custom-designed primers (Eurofins Genomics, Sigma-Aldrich) and PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma) on a CFX Connect Real-Time PCR machine (Biorad) ...
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA the coding sequence for Reb1 was amplified by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Systems Biology 2021Quote: ... 15 µL from each PCR product were mixed with 5 µL 6x loading dye solution (Sigma) before being loaded onto a 2% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Paleontology 2019Quote: 3µl of bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Neuroscience 2021Quote: ... DNA-free isolation was verified by PCR amplification (RedTaq® Polymerase, catalog no. D4309, Sigma-Aldrich). For this purpose ...
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bioRxiv - Microbiology 2022Quote: ... After amplification PCR products were checked on 1% (w/v) agarose gel (Sigma-Aldrich, Darmstadt, Germany) (5 µL of each PCR product mixed with 1 µL 6x loading dye for 50 min at 120 V ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... PCR-grade H2O and RedTaq Ready Mix according to the manufacturer’s instructions (Sigma-Aldrich, Rehovot, Israel). The amplified products were subjected to electrophoresis on 1.2% agarose gel containing agarose in 0.5 X TBE buffer and were stained with ethidium bromide ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR products were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). Cleaned PCR2 products were quantified using Qubit® dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed in 25 µl with JumpStart RED Accutaq LA polymerase (Sigma-Aldrich, cat# D8045) in the supplied buffer ...
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bioRxiv - Cell Biology 2023Quote: TRPM8 sequences were amplified by PCR using the following primers (ordered from Millipore Sigma, Burlington, MA):
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... Splicing by overlap extension PCR was performed with the KOD Xtreme Hot Start DNA Polymerase (Novagen). Generally ...
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bioRxiv - Genomics 2023Quote: ... The amplified cDNA was purified using the HighPrep PCR Clean-up System (Sigma Aldrich AC-60050) adding a volume of magnetic beads equal to 0.8x the sample volume ...
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bioRxiv - Immunology 2024Quote: ... 80µl of plasma per sample was plated in a 96-well PCR plate (Sigma Aldrich #EP951020401), frozen ...
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bioRxiv - Microbiology 2024Quote: ... PCR mixture was composed of 7.5 µL of JumpStart™ REDTaq® ReadyMix™ (Sigma-Aldrich), 0.75 µL of each primer 10 µM (515F and R926[24]) ...
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bioRxiv - Genomics 2021Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were mounted in Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Genomics 2022Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). To improve the efficiency of Tbr2 detection ...
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bioRxiv - Developmental Biology 2024Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were analyzed via confocal microscopy using a Nikon A1R+ ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei extraction was performing using the Nuclei Isolation Kit: Nuclei PURE Prep Kit (Millipore Sigma) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Synthetic Biology 2019Quote: We used the commercial kit: myTXTL from Arbor Biosciences (Sigma 70 Master Mix Kit, (USA). We used both our plasmid (10 nM final concentration ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Physiology 2022Quote: ... cells were washed two times with PBS+/+ and medium was replaced with glucose-free DMEM (catalog n°D5030, Sigma-Aldrich, Saint-Louis, MO, USA) without phenol red supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed three times in 1× PBST prior to mounting on glass slides in glycerol with Mowiol 4-88 anti-fade agent (EMD Millipore Corp., Billerica, MA). When required ...
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bioRxiv - Synthetic Biology 2020Quote: ... Next day the overnight culture was diluted 1/100 times in fresh LB containing required concentration of appropriate antibiotic(s) and inducer(s) (IPTG,Sigma Aldrich; ATC, Sigma Aldrich) and was transferred to High aspect ratio rotating vessels (HARVs ...
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bioRxiv - Plant Biology 2020Quote: ... The beads were washed tree times with TBS solution and FLAG-HDA6 was eluted with 200 ng/µl of Flag-Peptide (Sigma-Aldrich, Cat No F3290) by incubation the resin with synthetic peptide rotating for 30 min at 4 °C.
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bioRxiv - Cell Biology 2022Quote: ... The sample was washed three times with PBS and permeabilized for 15 min with 0.01% (w/v) digitonin (Sigma-Aldrich, St. Louis, MO, USA) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... 90°C), incubation time (1 min, 2 min) and permeabilization status (with or without incubation in 0.05% Triton-X [Sigma] in PBS for 10 min) were tested (Figure 2B) ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies were added to each aliquot (equivalent to 100 ml of cell culture): 6μl anti- H3K56ac for the mid-S time point (Merck-Millipore, 07-677-IS (lot# 266732) or 10 μl anti- H3K56ac for the early-S time point (Active Motif ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS three times and then blocked in PBS containing 1% bovine serum albumin (BSA) and 0.25% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: Blood was withdrawn at the indicated times during high fat diet period and plasma lipid levels were enzymatically measured using ELISA (Sigma Aldrich, Zwijndrecht, the Netherlands).
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).