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Citations for Millipore Sigma :
3901 - 3950 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... plants were washed three times in ddH2 O before cell death staining in a 2.5 mM Evans blue solution (Sigma-Aldrich, lot #MKCH7958) dissolved in 0.1 M CaCl2 pH 5.6 for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with PBS and DNA content were visualized by DAPI (1 μM; Cat# D9542, Millipore Sigma, I h) staining at room temperature ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Developmental Biology 2023Quote: ... Blots were washed again 3 times for 10 min each and developed using Immobilon Luminata Crescendo Western HRP substrate (Millipore, Cat#WBLUR0500). Western blots were imaged and quantified using ImageLab (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... the coverslips were further rinsed with 1X wash buffer A two times 5 min and incubated with 1X amplification buffer (Sigma, Cat#DUO82011) for 1 hr and 40 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Glycine and Tris-HCl in the protein solution were replaced with PBS three times using centrifugation with Amicon Ultra-15 Centrifugal Filter Unit (50K) (Millipore, Cat# UFC905024). Protein concentrations were determined using a NanoDrop spectrophotometer (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The reaction was quenched by washing three times with the quenching buffer (containing 5 mM Trolox [Sigma-Aldrich, cat#238813-25G], 10 mM sodium ascorbate [Sigma-Aldrich, cat#A4034-100G] ...
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bioRxiv - Immunology 2023Quote: ... Membranes were again washed with TBS-T for 3 times and were incubated with Immobilon Forte Western HRP substrate (WBLUF0500, EMD Millipore Corporation). Images were captured using AI600 Imager from GE Healthcare Life Science.
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bioRxiv - Cell Biology 2024Quote: ... or 50 µM indolmycin (BioAustralis Fine Chemicals) for ∼ 50 times the delayed death IC₅₀) and supplemented with 5 µM GGOH (Sigma Aldrich, G3278) to induce delayed death while rescuing the prenylation defect ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed five times for 10 minutes each in TBS-T and detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were treated with 1:1000 DAPI in PBS for 10 minutes and then washed five times with 1X PBS before mounting on glass slides using FluorSave (EMD Millipore Sigma). The coverslips were left to dry at least one hour and imaged immediately or stored at 4ºC for later imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were then rinsed 3 times with PBS and mounted onto microscope slides with 5 μl Mowiol 4-88 (Sigma-Aldrich #81381). Cells were visualized and imaged with a Nikon Ti-Eclipse A1M Confocal Microscope using a 60X objective ...
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bioRxiv - Cell Biology 2023Quote: ... The stored samples were washed with PBS three times and were incubated in the staining solution (PBS containing 5mM of EDTA, 20 µg/ml of propidium iodide (Sigma-Aldrich #81845) and 200 µg/ml RNAse A (New England Biolabs #T3018-1) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were plated to be 80% confluent at time of use and were treated with 1 μg/mL doxycycline (Sigma Aldrich D9891) for 24 hours prior to use ...
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bioRxiv - Cell Biology 2024Quote: ... The cell hydrogel complexes were gently washed three times with PBS buffer and digested by joining collagen II enzyme (Sigma, #10270-106) in incubator at 39°C for 6 min until the cells in the hydrogel package become rounding and shedding ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were removed at different times (0, 15, 30, 45, 60 and 120 min) and were immediately fixed with 4% formaldehyde (Sigma, #F-1268), washed 3x with PBS for 5min ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dishes were washed 3 times in phosphate buffered saline (PBS) for 5 minutes each then permeabilized with 0.1% Triton X-100 (Sigma, St. Louis, MO) in PBS for 40 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Elastic stain kit (Verhoeff van Gieson) and Masson’s Trichrome kit (all from Sigma-Aldrich), each according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Elastic stain kit (Verhoeff van Gieson) and Masson’s Trichrome kit (all from Sigma-Aldrich), each according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HMGB1 levels were measured using a commercial kit (HMGB1 ELISA kit A76696, Sigma-Aldrich). The assay was performed as described in the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... BSA and lysozyme were quantified with a quantification kit (Rapid-kit, Sigma-Aldrich, UK) using GloMax Discover microplate reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: A colorimetric assay kit (Phenolic Compounds Assay Kit MAK365, Sigma-Aldrich, St. Louis, USA) determined the total phenol content ...
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bioRxiv - Molecular Biology 2020Quote: ... An RNeasy Mini kit (Sigma) was used to further clean up and concentrate the RNA which was eluted in a final volume of 30 uL of DEPC H2O ...
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bioRxiv - Biochemistry 2020Quote: ... The ammonia assay kit (Sigma) and relevant acetylated peptides (Peptron ...
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bioRxiv - Cancer Biology 2020Quote: ... The starter kit from SIGMA supplies all other necessary reagents for Duolink® PLA reactions ...
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bioRxiv - Bioengineering 2020Quote: ... Leukocyte (TRAP) Kit (Sigma Aldrich) was used to stain for TRAP ...
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bioRxiv - Neuroscience 2020Quote: ... a PAS kit (Sigma Aldrich) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteomics Grade Kit (Sigma-Aldrich) as previously described by Lavigne and colleagues [46] ...
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bioRxiv - Cell Biology 2019Quote: ... Immobilin chemiluminescence kit (Millipore, WBKLS0500) was used to develop the blot.
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bioRxiv - Microbiology 2021Quote: ... Proteomics Grade kit (Sigma-Aldrich) for LC-MS/MS sample preparation.
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bioRxiv - Microbiology 2021Quote: ... Proteomics Grade kit (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: ... TMR red kit (Sigma, # 12156792910) in PBS containing 1% donkey serum ...
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bioRxiv - Cell Biology 2023Quote: Golgi isolation Kit (Sigma GL0010) was used with some modifications for cultured Cells Golgi subcellular fractionation ...
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bioRxiv - Molecular Biology 2023Quote: ... a Thrombin CleanCleave Kit (Sigma) was used.
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bioRxiv - Immunology 2023Quote: ... Aconitase Assay Kit from Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Leukocyte kit (Sigma, #86R-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the Peroxisome Isolation kit (Sigma) was used to isolate peroxisomes from primary hepatocytes by referring to the protocol provided by Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Leukocyte (TRAP) Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: A TRAP staining kit (Sigma) was used in this experiment ...
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bioRxiv - Cell Biology 2023Quote: ... The hydroxyproline assay kit (Sigma) was used according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The MitoPotential kit (EMD Millipore) was used with the Muse Cell Analyzer following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Leukocyte kit (Sigma, #86R-1KT) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed 2 times with phosphate buffered saline (PBS [pH=7.2-7.6]; catalogue number P4417, Sigma-Aldrich, St. Louis, MO, USA), blocked with 200 µl per well casein blocking buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... then washed three times in PBT and stored at 4°C until staining the nuclei with Hoescht (Sigma-Aldrich, USA, 1 μg/ml). Images were acquired with the Zeiss LSM 800 confocal microscope ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...