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Citations for Millipore Sigma :
4051 - 4100 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Aortas were then washed in 78% methanol for 5 minutes under gentle movement for a total for 3 times before incubating in a 2% ORO solution (Sigma-Aldrich, St Louis, MO) for 60 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The analytes were identified by the matching criteria of retention time and sensor ratio measures to known standards (Sigma Chemical Co., St. Louis MO.) consisting of dopamine ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we first compared retention times and mass spectra to an authentic standard mixture (C7-C40) (Supelco® 49452-U, Sigma-Aldrich, St. Louis, MO) with CHC samples ...
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bioRxiv - Genetics 2020Quote: ... The identification of the FAMEs was carried out by comparison in the retention times of the peaks in the sample with the Supelco 37 FAME mix pure standard by Sigma Aldrich (product number CRM47885), from which a calibration curve was created to calculate the concentration of fatty acids.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and amido black stain and left for 10 min before extracting the shells and rinsing three times in deionized water and air drying (Sigma Aldrich, St. Louis, USA).
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bioRxiv - Genetics 2020Quote: The gonads were washed 2 times for a minimum of 10 minutes each and dehydrated in a series of glycerol (Sigma-Aldrich, Catalog #: G5516-1L) washes for 1 hour minimum each ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed in PBT four times and mounted on a coverslip coated with poly-L-lysine (#P1524-25MG, Sigma Aldrich, St. Louis, Missouri).
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with H33342 nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Magnetic beads containing FLAG-tagged protein were washed four times with FLAG wash buffer (20 mM HEPES, pH 7.4, 100 mM NaCl, 0.05% [w/v] digitonin [Sigma: D141], and 10% [w/v] glycerol) and eluted with FLAG elution buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed three times for 10 min each at room temperature with 1x Duolink In situ Wash Buffer B (Sigma Aldrich, cat. no. DUO82049) followed by two washes of 2 minutes with 0.01x Duolink In Situ Wash Buffer B ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with the primary antibody overnight at 4°C and washed at room temperature three times for 5 min each with 1x Duolink In situ Wash Buffer A (Sigma Aldrich, cat. no. DUO82049). The Duolink In Situ PLA Probes anti-rabbit PLUS (Sigma Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were washed three times with PBS and lysed with a RIPA buffer containing protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis. MO). Total protein concentration in the lysates was determined by bicinchoninic acid (BCA ...
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bioRxiv - Developmental Biology 2024Quote: ... cochleae were washed 3 times for 15 minutes in PBS and the sensory epithelium was dissected off and mounted (Sigma-Aldrich, Cat. No. F6182) on a glass slide ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with DPBS five times for 3 min each, and stained with 4’, 6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS) (Sigma, San Francisco, CA, USA) in the dark at room temperature for 8 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed multiple times with PBSTx before being incubated in FITC-conjugated goat anti-mouse secondary antibody (Sigma cat. #A6667, 1:200) for 2-5 hr at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Membranes were washed three times with 1X PBS and incubated with appropriate primary antibody (see table below) diluted in 4% BSA (Sigma; at. No. A9418-50G) in 1X PBS overnight at 4° C ...
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bioRxiv - Microbiology 2023Quote: ... at which time the oocytes were washed free of the sperm and moved to a drop of KSOM culture medium (Millipore Sigma, #MR-101-D) for overnight culture ...
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bioRxiv - Neuroscience 2023Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with PI (Sigma, P4170, 10 μg/ml) as a red nuclear fluorescence dye and ready for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Filters were washed three times with TTBS buffer and then incubated for 1 hour with secondary peroxidase-coupled anti-rabbit antibody (1:7000, Millipore, Cat #401393-2 ML). Immunostaining was revealed by enhanced chemiluminescence luminosity (Amersham Pharmacia Biotech ...
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bioRxiv - Immunology 2024Quote: ... Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech ...
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bioRxiv - Genetics 2024Quote: ... The purified peptides were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) and analytical HPLC (Millipore Chromolith RP-18e column).
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bioRxiv - Microbiology 2024Quote: ... cells then fixed for 5 min with 4% PFA followed by 3 times of PBS wash and then permeabilized with 0.1% of Triton X-100 (Sigma-Aldrich, Cat. no. X100-00ML) in PBS for 5 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with Hoechst nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
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bioRxiv - Bioengineering 2019Quote: ... and complement C5a levels in serum were quantified by ELISA (Histamine kit: Enzo Life Sciences, Farmingdale, NY; Complement C3a kit: Quidel, San Diego, CA; Complement C5a kit: Sigma-Aldrich, St. Louis, MO). Spleen tissues were homogenized with a FastPrep-24 instrument in RIPA buffer supplemented with EGTA ...
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bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) and DBNL(1-374) were amplified by PCR and cloned into pET28a vector (Novagen) between BamHI and NotI sites to obtain constructs for expression of his-tagged proteins in E ...
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed using gene-specific primers (Table S3) with Kapa SyBr Fast (Sigma-Aldrich, #KK4618) in an Applied Biosystem ViiA 7 real-time PCR system.
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bioRxiv - Microbiology 2019Quote: ... Quantitative PCR mixtures (10 μL) contained 5 μL Kapa SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich), 300 nM of the primer pair ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNA was subjected to qRT-PCR using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich) in a CFX96 Touch™ Real-Time PCR Detection System (Biorad ...
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bioRxiv - Bioengineering 2019Quote: ... PCR was performed using control primers beyond the homology regions with KODXtreme Taq polymerase (Millipore, Massachusetts, USA). Where indicated ...
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bioRxiv - Biophysics 2020Quote: ... The resulting 5 kbp PCR fragments (Bio-DNA-DIG) (1.7 µm in length) reacted with neutravidin (Sigma) at a ratio of 100 neutravidin/DNA for 24 h at 4°C and were subsequently coupled to the anti-DIG beads at a reaction ratio of 150 neutravidin-DNA/bead for 30 min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Open reading frames were PCR amplified (Phusion Hotstart II polymerase, ThermoFisher Scientific or KOD DNA polymerase, Millipore) from genomic DNA or BAC ...
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bioRxiv - Microbiology 2020Quote: ... All PCR used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). E ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified with 10-12 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used the 2xKiCqStart® SYBR® Green qPCR ReadyMix™ (Sigma-Aldrich). PCR consisted of an initial activation step at 95°C for 3 min ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were digested with NdeI and BamHI and inserted into the pET-15b plasmid (Novagen) digested with the same enzymes ...
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bioRxiv - Biochemistry 2019Quote: Genotyping of founders (F0) was done using genomic PCR with the primers following primers provided by Sigma, forward 5’-CGCGATGACAGTTTCCAGTA-3’ reverse 5’-CAAACAAAAACCCACTGAGGA-3’ ...
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bioRxiv - Plant Biology 2019Quote: ... The synthesized sequences were amplified via PCR and cloned into the pET-15b expression vector (Novagen, www.merckmillipore.com) using NdeI and BamHI restriction sites to create expression constructs with an N-terminal 6-His-Tag followed by a thrombin cleavage site (pET-15b-SAP54 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the NdeI and KpnI sites of the pCOLADuet-1 (Novagen, USA) MCS-2 using the In-Fusion HD cloning kit.
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the BamHI and NotI sites of the pCOLADuet-1 (Novagen, USA) multiple cloning site (MCS)-1 ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were carried out using FastStart™ High Fidelity DNA polymerase (Sigma-Aldrich Cat no. 3553400001) in a reaction volume of 250 μl using 10 μl of the cDNA product as a template ...
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bioRxiv - Molecular Biology 2020Quote: ... An open-reading frame of the truncated Pch2 was PCR-amplified and inserted into pET15b plasmid (Novagen) in which an N-terminus of the PCH2 gene was tagged with hexa-histidine ...
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bioRxiv - Microbiology 2022Quote: ... the native encoding sequence was amplified by PCR and inserted into NdeI- and XhoI-digested pET36b(+) (Novagen). The fusion construct was verified by DNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... genotype was assessed with tail DNA extracted using RED Extract-N-Amp PCR Ready Mix (Sigma-Aldrich) followed by a polymerase chain reaction (PCR ...
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bioRxiv - Biochemistry 2024Quote: ... The coding sequence of the DDX1 protein was PCR amplified and inserted into the pET28 vector (Novagen). A His-tag was placed in the N-terminal of DDX1 and a stop codon was inserted at the coding sequence of amino acid 708 ...
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bioRxiv - Genetics 2023Quote: ... we subjected the amplified PCR products to electrophoresis using gels consisting of 2-3% agarose (Sigma Aldrich) in 1 x Tris-acetate-EDTA (1 x TAE ...
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bioRxiv - Microbiology 2023Quote: Classical PCRs were performed using the RedTaq (WVR) and amplicons were visualized by SYBR Green stained (Sigma) agarose gel electrophoresis (1 to 2% agarose) ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were PCR-amplified with 12-13 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was assessed using a commercial kit: DNA quantitation kit e Fluorescence assay (Sigma Aldrich) in a 96-well plate ...