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Citations for Millipore Sigma :
3701 - 3750 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Cell Biology 2022Quote: ... MCF-10A cells were released from the G2/M block by washing three times with prewarmed drug-free medium and immediately treated with 10 µM proteasome inhibitor MG-132 (Sigma #474787) for 6h ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3 times with 0.05% Tween 20 in PBS and 3 times with PBS before the addition of o-phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich). Reactions were stopped by the addition of 1 M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... was washed three times by suspending in purified water and collecting by centrifugation prior to adding 600 mg of purified BSA (Millipore-Sigma) in 6 ml of purified water ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the cleared supernatant ON at 4ºC and then washed five times with BC200 with 0.05% IGEPAL CA630 (Sigma, St. Louis, MO). Bound material was eluted in loading buffer by heating for 10’ at 70ºC.
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bioRxiv - Neuroscience 2024Quote: ... Single-cell suspensions were obtained after mechanical trituration (10 times with both a p1000 and a p200 pipet) in sterile PBS with 1% bovine serum albumin (fraction V, Sigma-Aldrich). To ensure singlets ...
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bioRxiv - Cell Biology 2024Quote: ... the resulting saponin supernatant containing RBC and PV soluble proteins was retained and the parasite pellet was washed three times in PBS before solubilisation in 5% (v/v) Triton X-114 (TX-114) (Sigma-Aldrich) (0 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5% potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were pipetted up and down 50 times until complete disaggregation and neutralized by the addition of 40 μL of 1% cell culture grade BSA (Sigma # A8806) to avoid the formation of clumps after dissociation ...
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bioRxiv - Immunology 2024Quote: Bone marrow-derived macrophages were prepared from C57BL/6 and Rel-/-mice as described.68 Macrophages were activated on day 6 with lipid A for the time indicated in each experiment (Sigma-Aldrich). Nascent transcript RNA-seq was performed and the data were analyzed as described.68,69
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed three times in PBST solution for 10 min and mounted with the Fluoroshield mounting medium (F6182, Sigma-Aldrich). Images were acquired with a confocal microscope (AiryScan 900 ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed three times with DPBS for 5 minutes each and then treated with 0.1 M HCl (Sigma-Aldrich, #320331) diluted with DPBS for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... The product was purified four times by spin filtration in Amicon® Ultra 15 mL filter devices (MWCO 30 kDa) from Sigma-Merck Millipore at 6000 rpm for 5 min at room temperature and then lyophilized overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cover slips were washed three times with DPBS and mounted on glass slides with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich #F4680). Slides were dried at RT in the dark overnight before imaging ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... suspended cells collected at the indicated time points were isolated by centrifugation and rapidly reseeded on poly-L-lysine (Sigma, P4832)–coated coverslips for 3 min before fixation.
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Cancer Biology 2023Quote: ... At the indicated time point cells were washed twice with 1 mL of PBS w/o calcium or magnesium (D8537, Sigma-Aldrich) and removed from the plate using 200 μL of Trypsin-EDTA (0.25% ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Cancer Biology 2023Quote: Isolated adipocytes obtained as described above were washed in PBS three times and proteins were extracted from 500 μl of adipocyte with 500 μl 1X RIPA buffer (Sigma-Aldrich) supplemented with complete antiprotease tablet (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were washed multiple times with PBST (0.8% Triton-X 100 in PBS) and incubated in blocking buffer (10% NGS (Sigma-Aldrich, #G6767) and 4% BSA (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS three times for 5 minutes and mounted using Fluoroshield™ with DAPI (Sigma-Aldrich F6057) and covered with a coverslip.
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bioRxiv - Bioengineering 2023Quote: ... 100 mM NaCl with 0.1% n-Dodecyl-B-D-Maltoside to remove excess SDS by using an 30 kDa amicon centrifugal filter (1/10 dilution, 3 times; EMD Millipore) before a final overnight dialysis (1/500 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then rinsed three times in PBS for 5 min at room temperature and PBS was removed and slides were mounted in Fluorsave (Millipore; #345789) and allowed to cure overnight in a dark chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were cultured for times indicated in each experimental figure/legend with either 5 µM nutlin-3A (Millipore Sigma, #45-SML0580) to stabilize p53 activation ...
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bioRxiv - Biochemistry 2023Quote: SDS-PAGE gel bands were cut into 1×1 mm gel pieces and washed three times for 30 min by incubation with 400 µl 50% acetonitrile (ACN, Sigma-Aldrich) and 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with PBS for 5 minutes followed by DAPI staining (2 µg/ml in PBS, Sigma, D9564) for 5 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Blots were washed three times with TBST and then incubated with HRP-conjugated secondary antibodies (Millipore Sigma, AP160P and 12-348) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Immunology 2023Quote: ... Infected and mock controls were washed three times in PBS and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Millipore 20-188) at 2 x with protease inhibitor for western blot analysis.
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bioRxiv - Cell Biology 2023Quote: ... The beads were washed three time with washing buffer (0.1% Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were fixed in 4% PFA at the indicated times (D0: before adding the differentiation media, D5: 5 days in differentiation media) and stained using αSMA-Cy3 (Sigma, #C6198). Images were taken using a Nikon Eclipse Ni-U or Nikon SMZ18 microscope and Ds-Qi1MC or Ds-Ri1 camera (Nikon Instruments Inc.).
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bioRxiv - Microbiology 2023Quote: ... 2 were prepared by washing animals with M9 three times and then filtering through a 20 μm nylon filter (Millipore SCNY00020). This filtering was then repeated a second time ...
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bioRxiv - Biochemistry 2022Quote: Protease treatment of >30kDa FBS fraction was performed by treating 200 µL of large fraction (previously washed 3 times with 1X PBS) with 10 µL of 20 mg/mL Proteinase-K (#SAE0151, Sigma-Aldrich) at 37°C for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... sections were rinsed three times with PBS and the coverslips were mounted on glass slides with Mowiol® 4-88 (Sigma) mounting medium ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin nephropathy was induced in C57Bl6/NJ mice by a one-time IP injection of cisplatin (20 mg per kg body weight, Sigma-Aldrich) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by washing with 1XPBS two times for 2’ each and blocked again with Biotin (0.001%, Sigma-Aldrich, Merck, Overijse, Belgium) for 20’ and washed twice for 2’ in 1XPBS each ...
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bioRxiv - Cell Biology 2023Quote: Cell culture media were collected at specific time points and glycerol in media was measured using free glycerol reagent (Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 2 times with PBS (10 mins each) and finally mounted a slide covered with DAPI mounting media (#F6057, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 1x PBS and incubated until analysis in cell culture medium supplemented with gentamicin (Sigma, G1272). As positive control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... autologous plasma samples were taken from the top layer and the “buffy coat” was extracted and washed 3 times with 1x PBS (Sigma-Aldrich) at 300 x g ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich) before mounted ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed them six times for 10 min with 1 % Triton (Triton X-100, X100-100ML, Sigma Aldrich, Merck, Germany) in PBS (thereafter named 1 % PBST ...
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bioRxiv - Neuroscience 2023Quote: ... We washed the sections three times for 10 min in PBS prior to mounting them using flouromount mounting medium (Sigma-Aldrich). We acquired images using an inverted confocal microscope (Leica ...
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bioRxiv - Microbiology 2023Quote: Short chain fatty acid species were identified on the basis of precursor ion accurate mass measurements and retention times compared against SCFA commercial standards (Sigma Aldrich), and quantification was performed against calibration curves ...
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bioRxiv - Cell Biology 2023Quote: ... and cell numbers were counted every 24-96 hours for up to the total incubation time of 360 hours by trypsinising cells and staining them with 0.4% trypan blue solution (Sigma-Aldrich T8154).