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Citations for Millipore Sigma :
3601 - 3650 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cells were counted and plated at a density of 1 x 106 cells per well of a 6 well plate that was pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). For immunocytochemistry ...
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bioRxiv - Bioengineering 2021Quote: ... 250 μL of recovered solution was transferred into a 96-well plate and the recovered glucose concentration was measured using a glucose (GO) assay kit purchased from Sigma (GAGO20). Glucose recovery rate was defined by the following formula.
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf discs were cut from positive fluorescent plants using a 7 mm cork borer and placed with the abaxial side up in the bottom of transparent Nunc® 96-wells plates (Sigma).
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bioRxiv - Plant Biology 2020Quote: ... and the 3 lines that germinated poorly after stratification showed >97% germination when sown on plates containing 10μM Gibberellin A4 (SIGMA ALDRICH, G7276).
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of parasites at 0.3% haematocrit was added to a 96 well plate and stained with SYBR Green I (Sigma, 1:5000) in 100 µl (15 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Cancer Biology 2021Quote: ... Human melanocytes seeded at 1.0×105 – 2.0×105 cells/well density in 6-well plates were incubated in viral supernatant with 10µg/mL polybrene (Sigma, TR-1003) and centrifuged at 300xg for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μL of samples and standards were added to a 96-well plate and glucose was measured using the Glucose Oxidase (GO) Assay kits (Sigma, GAGO20). Total circulating sugars was measured from the trehalase-treated sample ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated at 34°C for 72 hours and the cells were fixed with 38% formaldehyde (#47608; Sigma-Aldrich) in a 0.15M saline solution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... expressing a stable CCNB1-GFP construct were plated into 384 well plates with 500 cells per well and reverse transfected with an esiRNA library (10ng, Sigma Aldrich) using Hiperfect transfection reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated onto collagen-coated plates in Opti-MEM for 3 hours and subjected to respective treatment conditions of either glucose (D-(+)-Glucose (Sigma Aldrich) or erythropoietin (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed and signals detected with SIGMAFAST™ o-Phenylenediamine dihydrochloride peroxidase substrate (Sigma Aldrich, St. Louis, MO, USA). ELISA plate signals were read at OD 405 nm ...
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bioRxiv - Microbiology 2020Quote: Bacterial plate counts were performed on lysogeny broth (LB-Miller) agar plates (10 g tryptone, 5 g yeast extract, 10 g NaCl, and 15 g agar (all from Sigma-Aldrich) in 1 L of Millipore water) ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Cell Biology 2021Quote: HUVEC were seeded at 5×104 cells or 2×105 cells in 24-well plates on coverslips coated with 10 μg/ml fibronectin (from human plasma, Sigma Aldrich) and incubated overnight in complete EBM-2 medium ...
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bioRxiv - Cell Biology 2020Quote: LT-HSCs were directly flow sorted as individual cells per well into retronectin pre-coated ultra-low attachment 96-well plates (Sigma-Aldrich) in serum-free medium (StemSpan SFEM ...
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Cell Biology 2021Quote: ... single cell clones were generated by serial dilution in 96-well plates and maintained in growth media with 200 μg/mL Zeocin and 5 μM methotrexate (MTX, EMD Millipore). CHO-SEAP CMV::XRCC6 ATM+ PRKDC+ was generated analogously to CHO-K1 ATM+ PRKDC+ ...
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bioRxiv - Immunology 2022Quote: ... After 3 days incubation at 37°C the overlay was removed and the plates stained with crystal violet solution (spatula tip (∼4 mg) crystal violet powder (Sigma Aldrich) solved in 30 ml 37% formalin and 120 mL PBS (Sigma Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of this cell suspension was added per well to a 24-well ultra-low attachment plate (Sigma CLS3473). To test the effect of blebbistatin on aggregate morphology ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures in the plates were then transferred by the liquid handler into glass-bottom 384-well microscope plates (Matrical Bioscience) coated with Concanavalin A (Sigma-Aldrich). After 20 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Cell Biology 2022Quote: ... Cells were transferred to black-walled 96-well plates (Falcon) 4 h before the assay and incubated with EBM or 200nM Staurosporine (Sigma Aldrich) for 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were plated into culture plates coated with 0.01% cell culture-tested polylysine solution (Sigma, molecular weight: 150,000–300,000 Da), and maintained at 37 °C in of 5 % CO2 in air at a relative humidity of 80 %.
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bioRxiv - Neuroscience 2022Quote: ... Dissociated cortical cells from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Physiology 2022Quote: ... 70 animals were placed onto each 50 mm tight-lidded petri plate with modified NGM containing 40 mM paraquat (or methyl viologen dichloride, from Sigma-Aldrich), 51 μM FUdR ...
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bioRxiv - Microbiology 2022Quote: ... microspores were seeded in 96-well tissue culture plates containing 200 μL Czapek-Dox medium (CZD, Sigma-Aldrich Chemie, Munich, Germany). These spores were confronted with P ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... UAS-CsChrimson/UAS-Aion) were grown in darkness on grape agar plates with yeast paste containing 5 mM all-trans-retinal (Sigma-Aldrich). Staged third instar larvae (96 h ± 3 h AEL ...
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bioRxiv - Molecular Biology 2022Quote: ... dissociated monolayer cells were seeded inside a 50uL Matrigel droplet on a 6-well clear polystyrene flat-bottom plate (Millipore Sigma) at a density of 250 cell/uL Matrigel in the indicated media conditions + 1uM 4OHT or an equivalent volume of DMSO at the time of seeding ...
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bioRxiv - Immunology 2022Quote: ... Streptavidin-ALP was added and incubated at room temperature for 1 hour followed by washing and treating plates with substrate solution containing BCIP/NBT (Sigma-Aldrich) until colour development is prominent ...
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bioRxiv - Microbiology 2022Quote: ... and 100 μl spread on either LB agar plates with or without specified antibiotics (2 μg/ml amikacin disulfate salt or 7 μg/ml nitrofurantoin (both Sigma-Aldrich) or 50% (v/v ...
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bioRxiv - Physiology 2022Quote: ... young adult animals were transferred to 1 mM auxin plates supplemented with 20 mM paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich). Animals were scored as in lifespan assays but twice a day ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Developmental Biology 2022Quote: ... were cultured in Feeder-free on pre-coated plates with 0.5% growth factor-reduced Matrigel (CORNING 356231) (vol/vol in PBS with MgCl2/CaCl2, Sigma-Aldrich D8662) in E8 medium (made in-house according to51 ...
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bioRxiv - Bioengineering 2022Quote: ... The two-post array supporting the newly formed hydrogels was then lifted out of the casting plate and transferred to a fresh 24-well plate containing Lonza growth medium supplemented with 2 g/L aminocaproic acid (Sigma-Aldrich) to inhibit fibrinolysis ...
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bioRxiv - Bioengineering 2022Quote: ... tumoroids were manually transferred into 12-well culture plates coated with a layer of 2% (w/v) agarose (A9539, Sigma-Aldrich) gel ...
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bioRxiv - Plant Biology 2021Quote: ... Screening of Arabidopsis mutants was performed with seedlings growing under standard media and transferred to plates containing 140 mM NaCl (Sigma-Aldrich) or 300 mM mannitol (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: Freshly isolated ficoll-density centrifuged PBMCs were plated at 0.5 – 1 x 106 in a 96-well V bottom plates and stained for viability with 100 µL of 66 µM of cisplatin (Sigma-Aldrich) for 5 minutes on ice ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunocytochemistry using primary polyclonal antibodies of phosphorylated Tau pSer214 (, rabbit, 1:1000 Sigma, USA) and NOS (Cayman Chemical,1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Total carbohydrate was determined by the Dubois phenol-sulfuric acid method (76) adapted to a 96-well plate assay with the Total Carbohydrate Assay Kit (MAK 104, Sigma-Aldrich), using D-glucose as the standard and absorbance read at a wavelength of 490 nm ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Cell Biology 2021Quote: U2Os cells were transfected with EB1-mNeon and seeded on poly-D-lysine−coated borosilicate cover glass in six-well culture plates (Sigma-Aldrich) 24 hr prior to imaging ...
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bioRxiv - Microbiology 2020Quote: ... coli ω7249 strain by electroporation and transformants were selected by tetracycline resistance on a LB plate supplemented with 50 µg mL-1 of diaminopimelate (DAP, Sigma-Aldrich) (30) ...
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bioRxiv - Molecular Biology 2021Quote: ... media was collected and used to measure secreted Cxcl1 protein on an ELISA plate according to the KC/Cxcl1 ELISA kit manufacturer’s instructions (Sigma-Aldrich #RAB0117). Absorbance values at 450 nm were measured with a Tecan plate reader ...
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bioRxiv - Microbiology 2021Quote: ... medium and on tryptic soya agar (TSA) plates (both Oxoid) supplemented with 10 μg/ml chloramphenicol (CAM) or erythromycin (ERM) (Sigma-Aldrich) when appropriate ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...