Labshake search
Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
-
bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OH TAM, Sigma-Aldrich, H7904), L-selenocystine (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
-
bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide ...
-
bioRxiv - Neuroscience 2019Quote: ... IMR-32 neuroblastoma were differentiated for 15-16 days using the above media supplemented with 5-Bromo-2’-deoxyuridine (BrdU; 2.5 µM; Sigma) and N6 ...
-
bioRxiv - Neuroscience 2019Quote: ... Slices were washed with PBS and incubated for 2 h in blocking solution (0.1 M PBS, 0.25% Triton, 5% normal goat serum, Sigma Aldrich). Tissue was treated with primary antibody ...
-
bioRxiv - Plant Biology 2019Quote: Detection of Ca2+ in stomata was performed using the Fura-2 AM dye (Sigma Aldrich, CAS 108964-32-5) (Jiang et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy] acetic acid (DIOA; Sigma-Aldrich) and VU0240551 (VU ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 10 glucose (pH 7.35) up to 5 min before being transferred to 2% agarose low gelling temperature (Sigma) at 32°C ...
-
bioRxiv - Biochemistry 2020Quote: ... After formation of the CAK-MAT1Δ219-THZ1 complex by incubation of 0.2 mg/mL CAK-MAT1Δ219 (approx. 2 μM) with 5 μM THZ1 (EMD Millipore) for 1.5 h at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... were added to 1 mL of a 0.1M solution of 5-norbornene-2-carboxylic acid in 0.1M phosphate buffer (pH 6.0) (all reagents purchased from Sigma-Aldrich). The solution was allowed to react at room temperature with intermittent vortexing ...
-
bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
-
bioRxiv - Neuroscience 2020Quote: ... mice were pulsed with 100 µg per gram of body weight of 5-Ethynyl-2’-deoxyuridine (EdU) (Sigma-Aldrich) at 24 hours before sacrifice by injection intraperitoneally.
-
bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
-
bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
-
bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
-
bioRxiv - Biophysics 2020Quote: Zebrafish larvae at 5 days post-fertilization (dpf) were immobilized in 2% low melting point agarose (LMA) (Sigma-Aldrich) on microscope slides ...
-
bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
-
bioRxiv - Cell Biology 2020Quote: ... all the cell lines stably expressing inducible lenti vector were induced with 2-5 μg/ml of doxycycline (Sigma) for 24–96 h.
-
bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons were cultured at a density of 5 × 104 cells.cm−2 on glass coverslips coated with 0.04% polyethyleneimine (Sigma, PEI) in Dulbecco⍰s Modified Eagle⍰s Medium (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... and NMDA receptor mediated PSCs were blocked with DL-2-amino-5-phosphonopentanoic acid (D-AP5; 50 μM; Sigma). The AMPA-R PSC frequency and amplitude was determined from a 1 min interval after the recording had stabilized (~10 min after wash-in of picrotoxin/D-AP5) ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
-
bioRxiv - Immunology 2022Quote: ... or 16.67µg Pam2CSK4 plus 2 mM 5-A-RU (as described in [45]) and 50 µM MGO (Sigma-Aldrich) in 50 µl (25µl per nares) ...
-
bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
-
bioRxiv - Cell Biology 2021Quote: African green monkey kidney Vero E6 cell and Colorectal Adenocarcinoma human epithelial (Caco-2) cells were maintained at 37 °C in 5% CO2 in Dulbecco’s minimal essential medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... asynchronously growing subconfluent cells were labeled with 30 μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
-
bioRxiv - Pathology 2020Quote: ... the parasites (2 × 106/mL) were incubated with 200 µL L-arginine solution and 5 µM DAF-2DA (Sigma) for 2 h at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma Aldrich) in PBS for 15 min at 37 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
-
bioRxiv - Developmental Biology 2019Quote: ... rinsed again for 5 minutes in PBX and blocked for 30 minutes to 1h in 2% horse serum (Sigma) in PBS ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The material was then rehydrated by adding 1 × PBS for 5 min followed by incubation with 2% driselase (Sigma) for 60 min at 37°C in a moisture chamber to remove the cell wall ...
-
bioRxiv - Genetics 2020Quote: ... nematodes were cultured and then assayed for survival on plates with 10 μg/ml 5-fluoro-2-deoxyuridine (Sigma), to avoid vulval rupture [27] and eliminate live progeny ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were rinsed three times with assay buffer (8.3 g/L DMEM, 2 mM sodium pyruvate 5 mM dextrose, and 0.75 mM L-glutamine, pH 7.4, all from Sigma-Aldrich). Cells were then incubated at 37°C ...
-
bioRxiv - Bioengineering 2019Quote: ... devices were washed twice with DI H2O and then stained for 5 minutes with 2% alizarin red (Sigma-Aldrich) in DI H2O [pH 4.1-4.3] ...
-
CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... Membranes were blocked for 2 hours at room temperature in 5% dry milk in 1xPBS containing 0.05% Tween-20 (Sigma) and probed with anti-FLAG antibody (1:2,000) ...
-
bioRxiv - Developmental Biology 2021Quote: ... total RNA was isolated from young adult fruit flies (2-5 days) or S2 cells using TRI Reagent (Sigma) according to the manufacturer specifications ...
-
bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
-
bioRxiv - Immunology 2021Quote: ... via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA, Sigma). Mice were bled 14 days after the boost ...
-
bioRxiv - Immunology 2021Quote: ... the γ-secretase inhibitor (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester (DAPT; R&D) or dimethyl sulfoxide (DMSO; Sigma) was added to media at the concentrations indicated.
-
bioRxiv - Cell Biology 2020Quote: ... They were stained with 2% uranyl acetate for 5 min and embedded in 0.17% uranyl acetate and 0.33% polyvinyl alcohol (P8136, Sigma-Aldrich). After drying up ...
-
bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
-
bioRxiv - Cell Biology 2022Quote: ... (2×103-5×103) siRNA reverse transfected endothelial cells (24h or 48h post transfection) were seeded to fibronectin (Sigma)-coated E-plates (growth ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
-
bioRxiv - Immunology 2023Quote: PBMCs were washed twice and adjusted to 2×106 cells/ml in HBSS plus 5% FBS (Sigma-Aldrich F2442). To induce apoptosis ...
-
bioRxiv - Developmental Biology 2024Quote: ... exponentially growing cells were pulse-labeled for 20 minutes with 25 µM 5-iodo-2-deoxyuridine (I7125, Sigma-Aldrich), followed by a second 20-minute pulse with 250 µM 5-chloro-2-deoxyuridine (C6891 ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized and administered fluorescein isothiocyanate (FITC)–dextran (0.1 ml, 2 M MW, 5% w/v, Sigma-Aldrich) via tail veil to label blood vessels ...
-
bioRxiv - Immunology 2022Quote: ... cell suspensions were washed and stored in PBS containing 2% FBS (Omnilab) and 5 mM of EDTA (Sigma-Aldrich) for acquisition ...
-
bioRxiv - Bioengineering 2024Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...