Labshake search
Citations for Millipore Sigma :
3451 - 3500 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
-
bioRxiv - Cell Biology 2021Quote: ... the cell pellet was resuspended and inverted 4 times in 20 mL of chilled 36% iso- osmotic percoll gradient solution (P1644, Sigma-Aldrich) (percoll gradient solution ...
-
bioRxiv - Biochemistry 2020Quote: ... the solution was diluted five times with 100 mM ABC buffer and a further digestion with 1:50 dimethylated Sus scrofa trypsin (Sigma Aldrich) was carried out overnight at RT ...
-
bioRxiv - Plant Biology 2021Quote: ... The sections were washed three times with PBS and then digested using a cell wall digestion enzyme solution containing: 1% Driselase (Sigma-Aldrich), 0.5% cellulase (Sigma-Aldrich) ...
-
bioRxiv - Systems Biology 2020Quote: ... DNA-primary antibody conjugates were washed with 1× PBS four times and concentrated using 50 KDa Amicon Ultra Centrifugal Filters (Millipore, UFC505096). The concentration of conjugated oligo DNA and antibody with BCA Protein Assay Kit (Thermo Scientific 23225 ...
-
bioRxiv - Microbiology 2021Quote: ... the plates were washed three times with PBS-T (see above) and were blocked with 200 μl of 1% (w/v) BSA (Sigma Aldrich) in PBS-T for one hour at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins in the serum were removed by passing it through a pre-rinsed (7 times washed) Amicon Ultra-2ml 3000 MWCO (Merck Millipore, USA) column ...
-
bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
-
bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2019Quote: ... Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich, P4707) for one minute ...
-
bioRxiv - Microbiology 2021Quote: ... were washed three times in PBS and resuspended to 5% (vol/vol) in DMEM high glucose without Phenol Red (Sigma, Germany). Bacterial cultures were equalized to 1×108 in 500 µl and added to 500 µl sheep RBCs (5% vol/vol ...
-
bioRxiv - Cell Biology 2020Quote: ... The presence of fatty acid was determined by quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) analyses and comparison with a stock solution (Sigma-Aldrich) composed of four different fatty-acid standards (lauric acid [12:0] ...
-
bioRxiv - Biochemistry 2022Quote: ... The collected fluorescent protein was washed with a PBS buffer for 5 times under 100KD cut off centrifuge filter (Vivaspin) to remove the excess DDM at 6000 rpm (Sigma 3k15). Then the collected fluorescent protein was passed through a 300KD cut off centrifuge filter (Vivaspin) ...
-
bioRxiv - Molecular Biology 2022Quote: Human hPSCs cultured on MEFs were harvested using collagenase IV as big aggregates and settled 3 times in washing media (DMEM [Thermo Fisher Scientific], 5% Newborn Calf Serum [Sigma] ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 48 h (after that time, fresh media was added), palbociclib (1 μM) (PD033299, #S1116 Absource Diagnostic) or nutlin-3A (5 μM) (Sigma, #SML0580), as indicated ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed three times for 5 min in PBS and then mounted with DAPI mounting medium (Fluoroshield with DAPI, Sigma-Aldrich). Images were acquired with an inverted Leica DMIRE2 microscope and a Hamamatsu Camera ORCA Flash 4.0 LT.
-
bioRxiv - Genomics 2022Quote: ... beads were washed two times in RBD and resuspended in RBD supplemented with 1 mM PMSF and 1:100 dilution of PIC (Sigma P8340). Chromatin was added to tubes and allowed to incubate at 4°C with rotation overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were washed three times with PBS for 10 min at room temperature and incubated in primary antibody (anti-αSMA, Sigma, C6198) or anti-HuR ...
-
bioRxiv - Molecular Biology 2022Quote: ... the detected potential biomarkers were confirmed manually by spectra evaluation and comparison to the fragmentation pattern and retention time of commercial standards or online library mzCloud™ and commercial standards by Sigma. Adjusted P-value < 0.05 was reported as significant.
-
bioRxiv - Developmental Biology 2022Quote: ... Fixed tissues were rinsed three times with PBST before incubation in blocking buffer (PBS with 5% goat serum; Sigma Aldrich, #G9023) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... After three times washing with 1% PBS-T brains were dehydrated through an ethanol-xylene series and mounted in DPX Mountant (Sigma-Aldrich). Imaging was carried out using a Zeiss LSM 780 confocal microscope with 25x or 63x objective (oil) ...
-
bioRxiv - Microbiology 2020Quote: ... Coverslips were washed 3 times with PBS and mounted on a microscope slide with parafilm gasket in 1% (v/v) β-mercaptoethanol (Sigma), 150 mM Tris ...
-
bioRxiv - Cell Biology 2021Quote: ... sperm cells were washed with HS medium by centrifugation two times at 700 x g for 2 minutes to remove BAPTA-AM and eluted with HTF medium (EMD Millipore). Eluted sperm in HTF was incubated at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4OHT treated cells were washed 3 times with pre-warmed PBS and further incubated with 500 µg/mL auxin (Sigma; I5148). For transcriptional inhibition ...
-
bioRxiv - Biophysics 2021Quote: ... for 1 hour at room temperature and washed 3 times with PBS-T before mounting in 1% low melting point agarose (T9284, Sigma-Aldrich) followed by light sheet imaging ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... The tissue was then washed four times in 1x PBS with 1% Triton X-100 (#X100, Sigma Aldrich St. Louis, Missouri) (PBT ...
-
bioRxiv - Immunology 2020Quote: ... Then the slips were washed with PBS for three times, and after that the hemocytes were stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/ml) (Sigma, USA) for 5 min [55] ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were stained for DAPI diluted in water for 2 min and washed 3 times with water before drying cells on poly-L-lysine (Sigma, P8920) coated coverslips at room temperature protected from light ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples processed for immunohistochemistry using antibodies against NFIB were fixed for a shorter period of time (10 minutes) and were pre-treated with 1% (w/v) saponin (Sigma-Aldrich) in phosphate-buffered saline (PBS ...
-
bioRxiv - Biophysics 2021Quote: ... the cells were washed 3 times with 500 µl of PBS and then were fixed and permeabilized with ice-cold 100% methanol (Sigma Aldrich) for 15 minutes at -20°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were washed for ten minutes three times in PBT (0.2% Triton X, T8787; Sigma-Aldrich and PBS, P3813; Sigma-Aldrich) on an orbital rocker (SK-O180-S ...
-
bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
-
bioRxiv - Bioengineering 2020Quote: ... Plates were washed with phosphate-buffered saline with 0.1% Tween-20 (PBST) four times and then blocked with 2% bovine serum albumin (BSA, Sigma, #B2064-50G) in PBST for 2 hours at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... Plates were washed with phosphate-buffered saline with 0.1% Tween-20 (PBST) four times and then blocked with 2% bovine serum albumin (BSA, Sigma, #B2064-50G) in PBST for 2 hours at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: Blots were washed three times for 15 minutes with PBS-T and probed 45 minutes with secondary antibodies (rabbit anti-mouse (Sigma A4312), 1:10000 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... Pioneer and overall RNA replication levels were measured by performing infections at a MOI of 0,1 to 0,5 for different times points in the presence or absence of 100 μg/mL of chycloheximide (CHX, Sigma, cat. 4859). This compound blocks the translation of viral proteins from positive strand viral RNAs ...
-
bioRxiv - Cell Biology 2022Quote: ... High-pressure freezing at specified time points was performed in cryo-protectant (PBS with 2% Type IX ultra-low melt agarose (Sigma-Aldrich), 100 mM D-mannitol ...
-
bioRxiv - Cancer Biology 2022Quote: ... inhibitors were added a few hours after seeding. Media were replaced three times a week. For actin perturbation we used (Fig. 4) 1 μM Latrunculin A (Sigma-Aldrich); 1 μM Cytochalasin D (Sigma-Aldrich) ...
-
bioRxiv - Pathology 2022Quote: ... at room temperature for 10 minutes and washed 3 times in PBS containing 0.1 % v/v Triton X-100 (Sigma-Aldrich X100), blocked for 30 min using 10 % v/v chicken serum (Gibco 16110082 ...
-
bioRxiv - Neuroscience 2022Quote: ... with nerves from 3 mice pooled per time point in ice-cold PBS with 1% protease inhibitor cocktail (Sigma-Aldrich, P8340). Samples were minced ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed two more times with PBS (Biotech, PD8117) then blocked and permeabilised using a 3% BSA (Sigma-Aldrich, 1073508600), 0.2% Triton X-100 (VWR ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were washed three times and then incubated with thrombin (for LC3B/GFP-LC3; 10 μL of thrombin [Sigma-Aldrich; T6884] ...
-
bioRxiv - Bioengineering 2019Quote: ... Scaffolds were thoroughly washed 5 times with water and then incubated with 4mg/ml N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich) and 10mg/ml N-hydroxysuccinimide (NHS ...
-
bioRxiv - Cancer Biology 2019Quote: ... Sections were washed 3 times for 5 min in PBS and then cover-slipped and mounted using Fluoroshield mounting medium with DAPI (Sigma Aldrich). Stained sections were imaged on a Zeiss Axio Observer wide-field fluorescence microscope.
-
bioRxiv - Synthetic Biology 2019Quote: ... At 1 h post inoculation (time 0 in Figure 3) each flask was added with the stated amount of L-arabinose (Millipore Sigma): 0% ...
-
bioRxiv - Bioengineering 2019Quote: ... Samples (lysed samples from ALP activity assay or frozen samples after time point collection) were incubated overnight at 56 °C in Proteinase K solution (1mg*ml-1 Proteinase K (Sigma-Aldrich) in Tris/EDTA ...
-
bioRxiv - Bioengineering 2019Quote: ... The molecular mass of detected peaks was estimated by comparison to the elution times of PEG analytical standard (ReadyCal Set, #02393, Sigma-Aldrich) and of commercial HA with known molecular mass (2-2.2 MDa ...
-
bioRxiv - Immunology 2019Quote: ... and seeded (2×105/well at memory time point or 2.5 x105/well at effector time point in respective experiments) in serum-free unbuffered RPMI 1640 medium (Sigma-Aldrich # R6504) onto Cell-Tak (#354240 ...
-
bioRxiv - Plant Biology 2019Quote: ... The seeds were then washed 10 times with sterile distilled water and sown on MS agar plates (Sigma, St. Louis, MO) supplemented with 1% sucrose ...