Labshake search
Citations for Millipore Sigma :
3301 - 3350 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... bacterial cells from a 20 ml culture were collected by centrifugation (8000 x g, 5 min.) and washed two times with PBS pH 7.2 buffer (Sigma-Aldrich). Washed bacterial cells were dissolved in lysis buffer (1% SDS ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were then washed three times in distilled water and spun down in low melting agarose (2% in H2O; Sigma), let to solidify on ice ...
-
bioRxiv - Genomics 2023Quote: ... Excess dye was removed by washing conjugate with sterile DPBS 10 times using 30kDa Amicon filters (cat. UFC503024, EMD Millipore) at 12,000rpm ...
-
bioRxiv - Developmental Biology 2023Quote: ... washed three times in PBS/2%BSA for 5 min and then permeabilized using 0.1% Triton X-100 (Sigma-Aldrich) for 20 min followed by another washing cycle ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were washed three times with TBS and nuclei were stained with TBS containing 1.7 μg/mL DAPI (Sigma-Aldrich) for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... The beads were washed three times in IP buffer and eluted twice in 20 µl IP buffer with 100 ng/µl FLAG-peptide (Sigma) for 15 min at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... The chromatographic peak corresponding to ascorbic acid in the samples was identified by comparing its retention times with those of the standard (Sigma). Concentrations were calculated using the calibration curve generated from standard solutions prepared for the standard.
-
bioRxiv - Molecular Biology 2022Quote: ... the membranes were washed with PBS three times and then incubated with secondary antibodies: horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG antibodies (Millipore, Billerica ...
-
bioRxiv - Developmental Biology 2022Quote: ... The wells were washed with ddH2O for 3 times and incubated with 100ul/well of 10μg/ml fibronectin bovine plasma (Sigma-Aldrich) in 0.1% gelatin for 2 hours on an orbital shaker at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with ice-cold PBS and incubated with 0.25 µg/mL biotin hydrazide (66640-86-6, Sigma-Aldrich) in cold PBS for 1 hour on ice ...
-
bioRxiv - Microbiology 2023Quote: ... they were washed 3 times with 1M sorbitol and resuspended in RPMI 1640 broth containing protease inhibitor cocktail (Sigma-Aldrich) to final concentration of 3×108 cells/ml ...
-
bioRxiv - Immunology 2023Quote: ... The plates were washed a final time (5x with PBS-T) and the plate was allowed to develop by addition of AP substrate (Sigma). Optical density at 405 nm was measured in 5 min intervals ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were washed 5 times in PB 0.1 M and mounted with a mounting solution (Mowiol 4-88, Sigma-Aldrich).
-
bioRxiv - Biochemistry 2023Quote: ... Anti-Flag M2 beads were washed five times with 1 ml buffer A and then eluted with buffer A supplemented with 3xFlag peptide (Sigma). Unphosphorylated ACKR3 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
-
bioRxiv - Immunology 2023Quote: ... The protein was then eluted in 15 μL of peptide elution buffer three times using Flag peptide or HA peptide (Sigma) 300µg/mL ...
-
bioRxiv - Neuroscience 2023Quote: ... We then washed the slices 5 times in PB 0.1 M and incubated them with primary antibody rabbit IgG antiGABA (Sigma A2052) diluted in PB 0.1 M 1:1000 with 1% NGS overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides were stained with 500 times diluted DNA intercalator Ir193 (Standard BioTools Inc.) and 5 µg/ml DAPI (Sigma-Aldrich) for 1 hour at RT ...
-
bioRxiv - Plant Biology 2023Quote: Root tips of GFP fused protein transformed Arabidopsis were transferred onto slides and covered with a drop of 100 times diluted Propidium Iodide (PI) solution (1.0 mg/mL) (Sigma-Aldrich) for one minute ...
-
bioRxiv - Cancer Biology 2023Quote: ... The resulting conjugated antibodies are then washed for 3 times in high salt PBS (0.9 M NaCl) in a 50 kDa centrifugal column (Sigma, UFC505096), buffered with at least 30% v/v Candor Antibody Stabilizer (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... Diabetes was induced by one time for one day intraperitoneal injection of 110 mg kg−1 streptozotocin (STZ, Sigma Aldrich) dissolved in citrate buffer (pH 4.5)[36] ...
-
bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
-
bioRxiv - Microbiology 2023Quote: ... the reaction was stopped by FBS then cell were washed 2 time with PBS-based staining buffer containing 2% fetal bovine serum (FBS) and 0.01% NaN3 (Sigma-Aldrich), blocked Fc IgG receptors by pre-incubated with CD16/32(93 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed three times with FACS buffer and fixed in PBS containing 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 times for 5 min in PBS and blocked with 3% BSA in PBS-Triton 0.01% (Triton X-100, Sigma, T8787) for 1h ...
-
bioRxiv - Neuroscience 2023Quote: ... larvae were washed 3 times in PBS-T (phosphate buffered solution + 0.6% Triton-X 100) (Sigma-Aldrich, Cat. No. X100). PBS-T was removed and VECTASHIELD Mounting Media (Vector Labs ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were then washed three times in distilled water and dehydrated in acetone solution (Sigma, St Louis, MO, US) at graded concentrations (30%-40min ...
-
bioRxiv - Immunology 2023Quote: ... The cell cultures were washed three times with PBS and permeabilized with 100 μl 0.5% TritonX-100 (Sigma, Cat.#93443) at room temperature for 15 min ...
-
bioRxiv - Bioengineering 2023Quote: ... The grid was subsequently washed three times in 0.05 M phosphate buffer (freshly prepared using dihydrogen potassium phosphate (Sigma–Aldrich) and dipotassium hydrogen phosphate (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... The final solution was washed 3 times with 1X PBS using an Amicon Ultra 30 kDa MW cutoff centrifugal filter (#UFC903024, Millipore) to remove unreacted PMSF and then resuspended to 200 µL (same volume as original sample) ...
-
bioRxiv - Cell Biology 2023Quote: Brain sections were post-fixed with 4% PFA for 10 minutes and then washed with PBS three times and incubated 30 minutes with blocking solution (PBS/0.1% Triton X-100 containing 10% normal goat serum (Sigma-Aldrich)) and then incubation overnight at 4°C in blocking solution with primary antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were rinsed three times with 1xPBS and permeabilized with room temperature permeabilization buffer (0.3% IGEPAL CA-630 [Sigma-Aldrich; I8896] ...
-
bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
-
bioRxiv - Cancer Biology 2023Quote: ... Target cells were transduced sequentially two times and the transduced cell population was selected using 1 μg/ml puromycin (Sigma) as described previously 15.
-
bioRxiv - Neuroscience 2023Quote: ... the fibrils were washed three times with TBS to remove the excess TNM by using the 100 kDa MWCO filters (Millipore). The centrifugation steps were performed at 20,000 g for 30 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled SRM ...
-
bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
-
bioRxiv - Cell Biology 2023Quote: Sections were rinsed in 0.1 M PBS three times each for 10 min and then incubated with: i)1:1000 anti-NeuN (A-60; Merck Millipore), 1:300 anti-ΠIII-Tubulin (Tuj1 ...
-
bioRxiv - Genetics 2023Quote: ... Slides were washed three times in PBS before staining nuclei with 2 µg/mL Hoechst 33342 in PBS (Sigma Aldrich). Slides were washed once in PBS before mounting with DAKO mounting media (Agilent).
-
bioRxiv - Bioengineering 2023Quote: ... The gels were then washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% BSA (Sigma), 1% Triton X-100 (Sigma ...
-
bioRxiv - Plant Biology 2023Quote: ... Seeds were surface-sterilized by 70% ethanol and washed 4 times with sterilized ultra-pure water (Milli-Q water, Merck-Millipore) followed by stratification at 4 ºC for 2 d ...
-
bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
-
bioRxiv - Microbiology 2023Quote: ... the conjugated antibody was washed several times with W-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 50 kDa cut-off (Millipore-Sigma) and quantified using a NanoDrop spectrophotometer (280 nm) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The structures were then washed three times with PBS++/BSA (PBS supplemented with MgCl2 and CaCl2 (Sigma-Aldrich, #D8662-6X500ML) supplemented with 0.5% BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... basal OCR was measured three times followed by injection of 20 μl of 5 μM FCCP (Sigma-Aldrich, cat: #C2920) in port A to reach a final concentration of 0.5 μM ...
-
bioRxiv - Cell Biology 2023Quote: ... blots were again washed 3 times (5 min per wash in Tween-20 saline) before addition of ECL (Millipore, MA) for chemiluminescent detection on a BioRad Image Analyzer using Image Lab to capture and quantify signal intensity ...
-
bioRxiv - Biophysics 2023Quote: ... Sections were then washed three times for five minutes again in PBS and incubated in Sudan Black (0.1% for 10 minutes, 199664-25G, Sigma Aldrich). Removal of Sudan Black occurred with three washes in 30% ethanol (E7148-500ML ...