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Citations for Millipore Sigma :
3551 - 3600 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The identification of amino acids was based on retention times and m/z values obtained from amino acid standards (Sigma-Aldrich)
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bioRxiv - Neuroscience 2023Quote: ... The analytes were identified by the matching criteria of retention time measures to known standards (Sigma Chemical Co., St. Louis MO). Compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Microbiology 2023Quote: ... The resulting mixture was passed multiple times through a 0.45-μm Millex®-HV pore-size filter (Millipore, Billerica, MA, USA) to remove remaining prey ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in cold DPBS three times and lysed directly in the cell culture wells with RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Sigma-Aldrich). Lysates were cleared via centrifugation at 20,000 rcf at 4 °C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5 % potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 2 times with PBS (10 min each) and finally mounted onto slides covered with DAPI mounting media (#F6057; Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The analytes were identified by the matching criteria of retention time measures to known standards (Sigma Chemical Co., St. Louis MO). Compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Cell Biology 2022Quote: ... MCF-10A cells were released from the G2/M block by washing three times with prewarmed drug-free medium and immediately treated with 10 µM proteasome inhibitor MG-132 (Sigma #474787) for 6h ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3 times with 0.05% Tween 20 in PBS and 3 times with PBS before the addition of o-phenylenediamine dihydrochloride peroxidase substrate (Sigma-Aldrich). Reactions were stopped by the addition of 1 M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... was washed three times by suspending in purified water and collecting by centrifugation prior to adding 600 mg of purified BSA (Millipore-Sigma) in 6 ml of purified water ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the cleared supernatant ON at 4ºC and then washed five times with BC200 with 0.05% IGEPAL CA630 (Sigma, St. Louis, MO). Bound material was eluted in loading buffer by heating for 10’ at 70ºC.
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... suspended cells collected at the indicated time points were isolated by centrifugation and rapidly reseeded on poly-L-lysine (Sigma, P4832)–coated coverslips for 3 min before fixation.
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bioRxiv - Biochemistry 2024Quote: ... Cover slips were washed three times with DPBS and mounted on glass slides with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich #F4680). Slides were dried at RT in the dark overnight before imaging ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... The product was purified four times by spin filtration in Amicon® Ultra 15 mL filter devices (MWCO 30 kDa) from Sigma-Merck Millipore at 6000 rpm for 5 min at room temperature and then lyophilized overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed three times with DPBS for 5 minutes each and then treated with 0.1 M HCl (Sigma-Aldrich, #320331) diluted with DPBS for 5 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: Bone marrow-derived macrophages were prepared from C57BL/6 and Rel-/-mice as described.68 Macrophages were activated on day 6 with lipid A for the time indicated in each experiment (Sigma-Aldrich). Nascent transcript RNA-seq was performed and the data were analyzed as described.68,69
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed three times in PBST solution for 10 min and mounted with the Fluoroshield mounting medium (F6182, Sigma-Aldrich). Images were acquired with a confocal microscope (AiryScan 900 ...
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bioRxiv - Neuroscience 2024Quote: ... Single-cell suspensions were obtained after mechanical trituration (10 times with both a p1000 and a p200 pipet) in sterile PBS with 1% bovine serum albumin (fraction V, Sigma-Aldrich). To ensure singlets ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS three times for 5 minutes and mounted using Fluoroshield™ with DAPI (Sigma-Aldrich F6057) and covered with a coverslip.
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bioRxiv - Bioengineering 2023Quote: ... 100 mM NaCl with 0.1% n-Dodecyl-B-D-Maltoside to remove excess SDS by using an 30 kDa amicon centrifugal filter (1/10 dilution, 3 times; EMD Millipore) before a final overnight dialysis (1/500 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Cancer Biology 2023Quote: Isolated adipocytes obtained as described above were washed in PBS three times and proteins were extracted from 500 μl of adipocyte with 500 μl 1X RIPA buffer (Sigma-Aldrich) supplemented with complete antiprotease tablet (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were washed multiple times with PBST (0.8% Triton-X 100 in PBS) and incubated in blocking buffer (10% NGS (Sigma-Aldrich, #G6767) and 4% BSA (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: SDS-PAGE gel bands were cut into 1×1 mm gel pieces and washed three times for 30 min by incubation with 400 µl 50% acetonitrile (ACN, Sigma-Aldrich) and 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... autologous plasma samples were taken from the top layer and the “buffy coat” was extracted and washed 3 times with 1x PBS (Sigma-Aldrich) at 300 x g ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Systems Biology 2023Quote: ... Blots were washed three times with TBST and then incubated with HRP-conjugated secondary antibodies (Millipore Sigma, AP160P and 12-348) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then rinsed three times in PBS for 5 min at room temperature and PBS was removed and slides were mounted in Fluorsave (Millipore; #345789) and allowed to cure overnight in a dark chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were cultured for times indicated in each experimental figure/legend with either 5 µM nutlin-3A (Millipore Sigma, #45-SML0580) to stabilize p53 activation ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with PBS for 5 minutes followed by DAPI staining (2 µg/ml in PBS, Sigma, D9564) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 1x PBS and incubated until analysis in cell culture medium supplemented with gentamicin (Sigma, G1272). As positive control ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich) before mounted ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed them six times for 10 min with 1 % Triton (Triton X-100, X100-100ML, Sigma Aldrich, Merck, Germany) in PBS (thereafter named 1 % PBST ...
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bioRxiv - Immunology 2023Quote: ... Infected and mock controls were washed three times in PBS and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Millipore 20-188) at 2 x with protease inhibitor for western blot analysis.
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bioRxiv - Cell Biology 2023Quote: ... The beads were washed three time with washing buffer (0.1% Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were fixed in 4% PFA at the indicated times (D0: before adding the differentiation media, D5: 5 days in differentiation media) and stained using αSMA-Cy3 (Sigma, #C6198). Images were taken using a Nikon Eclipse Ni-U or Nikon SMZ18 microscope and Ds-Qi1MC or Ds-Ri1 camera (Nikon Instruments Inc.).
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bioRxiv - Microbiology 2023Quote: ... 2 were prepared by washing animals with M9 three times and then filtering through a 20 μm nylon filter (Millipore SCNY00020). This filtering was then repeated a second time ...
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bioRxiv - Biochemistry 2022Quote: Protease treatment of >30kDa FBS fraction was performed by treating 200 µL of large fraction (previously washed 3 times with 1X PBS) with 10 µL of 20 mg/mL Proteinase-K (#SAE0151, Sigma-Aldrich) at 37°C for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... sections were rinsed three times with PBS and the coverslips were mounted on glass slides with Mowiol® 4-88 (Sigma) mounting medium ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin nephropathy was induced in C57Bl6/NJ mice by a one-time IP injection of cisplatin (20 mg per kg body weight, Sigma-Aldrich) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by washing with 1XPBS two times for 2’ each and blocked again with Biotin (0.001%, Sigma-Aldrich, Merck, Overijse, Belgium) for 20’ and washed twice for 2’ in 1XPBS each ...
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bioRxiv - Cell Biology 2023Quote: Cell culture media were collected at specific time points and glycerol in media was measured using free glycerol reagent (Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 2 times with PBS (10 mins each) and finally mounted a slide covered with DAPI mounting media (#F6057, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... Brain slices were rinsed in PBS 3 times (5 min each) and then were stained with Hoechst (1:1000, #33342, Sigma-Aldrich) for 10 min ...