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Citations for Millipore Sigma :
3651 - 3700 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Genomics 2019Quote: ... We performed qRT-PCR using FastStart Universal SYBR Green Master Mix with ROX (Sigma, 4913914001) on a ViiA 7 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich, see Table 2, (Buenrostro et al., 2013) for 5 initial cycles (1 cycle ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with SacI und SalI and cloned into pET-24a (Novagen), resulting in plasmid pET24a-OLA1-6xHIS ...
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bioRxiv - Microbiology 2019Quote: ... Digested PCR product was ligated into a modified T7 promotor-based pET33b expression vector (Novagen) that contained the super-folding variant of green fluorescent protein encoding gene with C-terminal 6xHis-tag and a modified multiple cloning site ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were selected and screened by PCR using the Expand Long Range dNTPack (Sigma), PCR buffer supplemented with 2% DMSO and the primer pairs for the 5’ region (LR 5’ fw ...
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bioRxiv - Bioengineering 2022Quote: ... Fragments were amplified by PCR using Q5 polymerase and assembled into a pET28a(+) vector (Novagen) via Gibson Assembly using reagents from New England Biolabs (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Captured products were amplified through PCR using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore), cloned into the pET23-P2-D12A-THR expression backbone ...
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bioRxiv - Immunology 2022Quote: ... cDNAs were mixed with indicated gene-specific primers and SYBR green PCR Master Mix (Sigma), and qRT-PCR was performed on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Immunology 2022Quote: ... PCRs with 50 ng of prepared cDNAs were carried out using Jumpstart Taq polymerase (Sigma) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was cloned in the BglII/BamHI sites of pFLAG-CMV2 (Sigma-Aldrich). The A1625G variant was introduced by site-directed mutagenesis using primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloning the PCR product between the NdeI and BamHI sites of pET9a (EMD Millipore). PCR-amplified DNA sequences were confirmed by Sanger sequencing.
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bioRxiv - Genetics 2024Quote: ... The PCR product was cloned into the expression vector pET-30a (Novagen, Madison, WI, USA). The fusion peptide expression and purification were carried out as described previously (Miao et al. ...
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bioRxiv - Biochemistry 2024Quote: ... All PCR reactions were conducted using KAPA HiFi Hotstart DNA polymerase (Sigma-Aldrich, catalog #BK1000) unless stated otherwise ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then quickly washed with CO2 -balanced complete cell culture medium five times and fixed with 4% paraformaldehyde (PFA) (Sigma-Aldrich, 158127) in PBS for 15 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 3 times in PBS and placed in a conical tube with a 30% sucrose (Sigma-Aldrich, S3929-1KG) in PBS solution for 2-3 days until sinking to bottom of conical tube ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed in kit Buffer A 3 times for 15 minutes and incubated with the PLA probes Duolink In Situ PLA Probe Anti-Mouse PLUS (Sigma-Aldrich, DUO92001) and Duolink In Situ PLA Probe Anti-Rabbit MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times with PBS and blocked for 1 hour with 0.2% triton X-100 and 3% BSA (Sigma-Aldrich, Gillingham, UK) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed again 3 times for 5 minutes each in PBS then non-specific binding sites were blocked in 1% bovine serum albumin (Sigma-Aldrich, A9418) in PBS for approximately 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was quenched by washing the cells tree times with freshly prepared quench solution (PBS containing 10 mM Sodium ascorbate, 10 mM Sodium Azide, 5 mM Trolox (Sigma-Aldrich, 238813)) ...
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bioRxiv - Molecular Biology 2021Quote: ... the testicular cells were washed three times using the EKRB buffer followed by trypsin digestion by incubation in 10ml EKR buffer containing trypsin (Sigma, 0.25mg/ml) and DNase I (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Next day the overnight culture was diluted 1/100 times in fresh LB containing required concentration of appropriate antibiotic(s) and inducer(s) (IPTG,Sigma Aldrich; ATC, Sigma Aldrich) and was transferred to High aspect ratio rotating vessels (HARVs ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was then rinsed three times with TBST and visualized with UV lighting after applying the chemiluminescent substrate to the membrane (EMD Millipore #WBLUF0100).
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bioRxiv - Genetics 2020Quote: ... washed three times with 5 ml PBS and then 1 ml of 0.005% propidium iodide (PI) (P4864; Sigma-Aldrich, St Louis, MO) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Basal oxygen consumption was measured six times followed by three measurements after each addition of 1 μM of oligomycin A (Sigma #75351-gMG), 2 μM carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were next washed four times for 3 min in MilliQ water at room temperature and incubated in a 1% solution of thiocarbohydrazide (Sigma-Aldrich, USA) for 20 min at 60 °C ...
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bioRxiv - Genetics 2020Quote: ... blood glucose was measured using a glucometer from tail tip blood before and multiple times within 2 hours of intraperitoneal injection of 2g/kg D-(+)-Glucose (Sigma Aldrich # G7528).
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bioRxiv - Biophysics 2020Quote: ... Antibody conjugates were purified and washed up to three times using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML) to remove excess dyes ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed three times in PBS (10 min each) at room temperature and coverslipped in mounting medium (Fluoromount, Sigma Aldrich F4680). Immunofluorescence was then observed with a slide scanner (Zeiss ...
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bioRxiv - Systems Biology 2021Quote: ... underwent the standard media changes and then at time 0H treated with 10 ng/ml OSM and either a protease inhibitor cocktail at a 1:400 dilution (Sigma-Aldrich #P1860), 40 μM bestatin (Sigma-Aldrich # B8385) ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... washed one time with DPBS and then incubated with primary antibody against collagen type I in DPBS (1:1000, Sigma, cat# C2456) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Identity of glycosaminoglycan-derived disaccharides was inferred from retention time alignment with the major constituents of chondroitin sulphate sodium salt from shark cartilage (Sigma-Aldrich, C4384), and bovine trachea (Sigma-Aldrich #C9819).
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bioRxiv - Bioengineering 2021Quote: ... This suspension was freeze-thawed at least ten times using liquid nitrogen and extruded 11 times through a LIPEX extruder (Northern Lipids, Burnaby, B.C., Canada) with a 400 nm cutoff Millipore filter (Millipore Sigma, Burlington, MA).
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bioRxiv - Neuroscience 2020Quote: ... This step was repeated three times and the resulting pellet gently re-suspended in 1 mL of 27% Percoll (Sigma Aldrich, P4937) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... was added for 5 min at 37°C and cells were washed three times with cold PBS with Ca2+ before mounting with MOWIOL® 4-88 Reagent (Sigma-Aldrich). The detailed list of antibodies used is summarized in Table S4 ...
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bioRxiv - Microbiology 2021Quote: ... The grids were rinsed with the same buffer five times and then were incubated with anti-mouse IgG conjugated with 10 nm gold particles (Sigma, cat# G7652) at a 1:10 dilution in 1% BSA-PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were immersed in Neo-Clear® three times for 5 minutes and then mounted using Neo-mount® mounting medium (Merck Millipore), coverslipped and left to dry overnight in at 42°C.
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bioRxiv - Biophysics 2020Quote: ... cells were washed three times with phosphate-buffered saline with calcium and magnesium (PBS, PAN-Biotech) and fixed with 3.7% paraformaldehyde (Sigma-Aldrich, Munich, Germany) for 25 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed five times in PBS-T and blocked with buffer containing 5% milk (Difco #232100) and 1% BSA (Sigma #A906-500G) in PBS-T (BioVision #2310-100) ...
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bioRxiv - Cancer Biology 2022Quote: The cells were collected at the indicated time points and cDNA pools were prepared by extraction of total RNA from cell lines using Trizol (Sigma-Aldrich, T9424), followed by reverse transcription using MMLV reverse transcriptase (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed by sterile phosphate buffer saline (PBS) 3 times and then incubated with 5 mg/mL MTT (Sigma-Aldrich; #M2128) for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of SCFA in the chromatograms was determined based on the retention times of the respective SCFA standards (Sigma-Aldrich, United Kingdom) at concentrations ranging between 5 to 30 mM.
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bioRxiv - Neuroscience 2021Quote: ... The arrays were rinsed three times with distilled water before coating with laminin (5µg/mL in distilled water; Sigma-Aldrich, MO, USA) for at least 1h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... this media was combined with the media from the 48 hour time point and filtered through a 0.45 um syringe filter (Millipore SLHV033RB; BD 309653). One 15 cm dish was transfected for each 35 million K562 cells to be transduced.
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bioRxiv - Bioengineering 2021Quote: ... Wells were washed five times and incubated for 1 h with 1 µg/mL HRP-conjugated goat anti- human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Immunology 2021Quote: ... To perform modified gentamicin protection assays cells were treated at the indicated time points with 20 µg/mL gentamicin and 40 U/mL penicillin G (Sigma, PENNA-100MU) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...