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Citations for Millipore Sigma :
3401 - 3450 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... specific primers were designed and PCR was performed using KOD DNA polymerase (Sigma Aldrich). The amplification of DNA fragments was done following manufacturer’s instructions into a Bioer GeneExplorer thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Biophysics 2024Quote: ... 45 μL of proteinase K (PK) mix (20 μg PK (Sigma Aldrich, PCR grade), 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Donor DNA was amplified by PCR using the high fidelity KOD DNA polymerase (Novagen) to amplify a fragment containing the tag and the resistance cassette ...
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bioRxiv - Immunology 2024Quote: ... The PCR reaction was carried out by KAPA2G Fast ReadyMix™ (Sigma Aldrich, KK5103). Primers were designed targeting the intron 1 and intron 3 regions of the target DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were blocked with 10% FBS in PBS for 1 h at RT and incubated overnight at 4°C with a primary anti-γH2AX antibody (05-636, EMD Millipore, 1:500 in 10% FBS-PBS). Following primary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2020Quote: ... were reconstituted in ACN/IPA/H2O (65:30:5, v/v/v, 100 µL) by vortexing (2 × 30 s, RT) and transferred to an amber vial (Sigma; QSertVial™, 12 × 32 mm, 0.3 mL) for LC–MS analysis.
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bioRxiv - Biophysics 2022Quote: ... cells were washed 2x at room temperature (RT) with PBS containing calcium and magnesium pH 7.2 (PBS/Ca/Mg) (either made by hand or Sigma D1283 diluted to 1x and pH’d to 7.2) and incubated with 2 mM DSP (ChemScene CS-0068460 ...
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bioRxiv - Microbiology 2020Quote: ... Aspartate- and Coenzyme A Assay Kits (all kits obtained from Sigma Aldrich) utilizing the entire ...
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bioRxiv - Microbiology 2023Quote: ... Aspartate- and Coenzyme A Assay Kits (all kits obtained from Sigma Aldrich) utilizing the entire ...
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bioRxiv - Molecular Biology 2024Quote: ... A commercial cell proliferation kit (Millipore Sigma, Cell Proliferation Kit I (MTT)) was used for mesenchymal stem cells and endothelial cells ...
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bioRxiv - Biophysics 2021Quote: ... the nanopillar substrates were washed with PBS three times and incubated in PBS with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257). After further washing with PBS (3X) ...
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bioRxiv - Cell Biology 2020Quote: ... After cells adhered the first data point was read as time zero by adding 1:10 of cell proliferation Reagent WST-1 (Sigma, #11644807001) to cells and incubating them at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... After that time cells were lysed on ice by using HEPES based lysis buffer containing proteases inhibitor cocktail (1:200) (Sigma P8340). Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lineage-labeling was induced at indicated time points using fresh or pre-heated (65°C for 10 min) 4-Hydroxytamoxifen (4-OHT) (Sigma H7904) stock solutions in DMSO at a final concentration of 10 μM in E3 embryo medium (Felker et al. ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed three times with 1X TBS followed by water and mounted using Fluoroshield mounting medium with DAPI (Sigma, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were washed three times in PBS for 5min and incubated with biotinylated anti-rabbit IgG (1:500 in PBS, Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed three times with TBS-0.05% Tween®20 (TBS-T) and incubated with appropriate HRP-conjugated secondary antibodies (Millipore Sigma), one hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed four times in 1X PBS for 5 minutes and blocked with 2% Normal Donkey Serum (Millipore S30-100ml) before incubation with secondary antibodies in 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was isolated from iPSC cultures driven towards glutamatergic deep cortical neurons or GABAergic neurons at time points along the differentiation protocol spectrum (Days 0, 12, 35, 60, and 35+14; Trizol, Sigma-Aldrich). Total RNA was reverse transcribed and used for quantitative RT-PCR with primers specific to Sox2 ...
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bioRxiv - Cell Biology 2022Quote: ... After the incubation, the samples were washed four times with RIPA buffer (50 mM HEPES buffer, 500 mM LiCl (Sigma-Aldrich), 1.0 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with PBS-G and blocked in PBS-G with 0.5% fish skin gelatin (Sigma-Aldrich, G7041) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the plates were placed on ice and washed three times with PBS containing 10 mM EDTA (Sigma-Aldrich, 150-38-9). After the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were washed in PBS at least 3 times for 4 hrs and then incubated in Histodenz-Triethanolamine solution (about 80% Histodenz D2158 (Sigma-Aldrich), 11.5 mM PB ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Systems Biology 2019Quote: ... we harvested cells at indicated time points and isolated proteins by lysis in the presence of protease and phosphatase inhibitors (Roth and Sigma-Aldrich), Trichostatin A (APExBio ...
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bioRxiv - Physiology 2019Quote: ... was then applied and hMSCs were cultured for a further 24 h after which time cells were lysed with tri-reagent (Sigma Aldrich) and mRNA isolated as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... concentrated lentivirus was added to cells at the time of plating in the presence of 4 µg/mL Hexadimethrine bromide (Polybrene, Sigma-Aldrich) for an infection efficiency of about 80% ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS five times for 5 min each and incubated with DAB for 10 min and pararosaniline solution (HT203, Sigma-Aldrich) for 2 min ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed cells were then washed three times in 4 mL of Dulbecco’s phosphate-buffered saline (DPBS) and permeabilized using 0.2% Triton X-100 (Sigma-Aldrich, cat. T8787) in DPBS at 37°C for 20 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitates were then washed three times with cold Triton lysis buffer and were analyzed by western blot using an anti-His antibody (Sigma Aldrich).
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Immunology 2019Quote: ... the culture medium was collected and concentrated five times by ultrafiltration (Amicon Ultra-50 with MWCO 100K, Millipore, Bedford, MA, USA). Viral titers were measured by a kit (Lenti-X p24 Rapid titer kit ...
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Stained embryos were washed three times with 1x Holt’s buffer and then anesthetized with 0.02% w/v MS-222 (Sigma-Aldrich, cat. #A5040) before the analysis ...
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bioRxiv - Biophysics 2019Quote: ... The cells were washed in PBS three times and then reduced for 10 min with 50 mM NH4Cl (Sigma Aldrich, 254134), followed by three additional washes in PBS.
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bioRxiv - Biochemistry 2019Quote: ... Cells were cleaned in phosphatebuffered saline (PBS) for three times and stained in PI/FITC-Annexin V with 50 μg/ml RNase A (Sigma-Aldrich). Keep cells in dark processing at the room temperature for 1 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Peaks were tentatively identified by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Immunology 2019Quote: ... The microarray was then rinsed for 3 times with washing buffer and incubated with 200 μL of Horseradish peroxidase (HRP) conjugated Rabbit anti-Goat IgG (Sigma-Aldrich) diluted 1:20000 in Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Medium was removed and cells were incubated for another hour (total infection time: 2 h) at 37 °C in FBS-containing culture medium supplemented with 20 μg/mL gentamicin sulfate (Sigma-Aldrich), to kill extracellular bacteria ...
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bioRxiv - Immunology 2021Quote: ... the slips were washed with PBS three times and stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... the eluate was washed 3 times with PBS in an Amicon Ultra-4 Centrifugal Filter Unit (NMWL 3 kDa, Millipore, UFC800324) and finally concentrated to < 250 µl ...
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bioRxiv - Biochemistry 2021Quote: ... The worms were removed from the fish body cavity and washed two times in a solution containing RPMI-1640 medium (Sigma-Aldrich) with 1% antibiotic antimycotic solution (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Neuroscience 2020Quote: ... Organoids were washed with PBS three times for 10 min each at room temperature and then allowed to sink in 30% sucrose (Sigma Aldrich) at 4 °C ...