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Citations for Millipore Sigma :
3601 - 3650 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... embryos were pipetted up and down 50 times until complete disaggregation and neutralized by the addition of 40 μL of 1% cell culture grade BSA (Sigma # A8806) to avoid the formation of clumps after dissociation ...
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bioRxiv - Cell Biology 2024Quote: ... the resulting saponin supernatant containing RBC and PV soluble proteins was retained and the parasite pellet was washed three times in PBS before solubilisation in 5% (v/v) Triton X-114 (TX-114) (Sigma-Aldrich) (0 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5% potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and butyric acid) were identified and quantified based on the known standard retention times and peak areas (Sigma-Aldrich, MO, USA).
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bioRxiv - Neuroscience 2023Quote: ... These slices were then rinsed for 3 times in PBS (5 min each) and then stained nuclei with Hoechst (1:1000, #33342, Sigma-Aldrich) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... We then washed the cells three times with PBS and blocked them with 5% bovine serum albumin (BSA; Sigma; Cat #A3983) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Microbiology 2023Quote: Short chain fatty acid species were identified on the basis of precursor ion accurate mass measurements and retention times compared against SCFA commercial standards (Sigma Aldrich), and quantification was performed against calibration curves ...
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bioRxiv - Neuroscience 2023Quote: ... We washed the sections three times for 10 min in PBS prior to mounting them using flouromount mounting medium (Sigma-Aldrich). We acquired images using an inverted confocal microscope (Leica ...
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bioRxiv - Cell Biology 2023Quote: ... and cell numbers were counted every 24-96 hours for up to the total incubation time of 360 hours by trypsinising cells and staining them with 0.4% trypan blue solution (Sigma-Aldrich T8154).
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bioRxiv - Cancer Biology 2023Quote: ... At the indicated time point cells were washed twice with 1 mL of PBS w/o calcium or magnesium (D8537, Sigma-Aldrich) and removed from the plate using 200 μL of Trypsin-EDTA (0.25% ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Plant Biology 2024Quote: ... Tissue was harvested and snap-frozen in liquid nitrogen at the indicated time points before RNA extraction using using Tri Reagent® according to the manufacturer’s protocol (Sigma Aldrich, Dorset ...
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bioRxiv - Physiology 2024Quote: The CaN KD and Ctrl KD cells were rinsed three times with phosphate-buffered saline (PBS) and then stimulated with 1 μM Ionomycin (I9657, Sigma-Aldrich) for 30 s in the presence of 1X PBS containing 0.5 mM CaCl2 ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich). Human serum albumin (0.1 µg ...
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bioRxiv - Neuroscience 2024Quote: ... OXY and AVP immunohistochemical labeling was performed at the same time using as first antibodies mouse anti-OXY monoclonal antibody (1/1000, Millipore MAB5296) and T-5048 Guinea pig anti (Arg8)-vasopressin antibody (1/1000 ...
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bioRxiv - Immunology 2024Quote: ... Wells were then washed 6 times with PBS/T and developed with 100 µL of p-nitrophenyl phosphate substrate at 1 mg/mL (Sigma-Aldrich) and optical density read at 405 nm (OD405 ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Cover slips were washed three times with 1 ml PBS and mounted on microscopy slides with Fluoroshield mounting medium (Sigma-Aldrich). Images were taken with the Axio Observer Z1 (Zeiss ...
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bioRxiv - Microbiology 2024Quote: ... by human neutrophils was measured over a time of 30 minutes by monitoring luminol-amplified chemiluminescence using 282 μM luminol (Sigma Aldrich). Neutrophils were stimulated either with negative control (Hank Balanced Salt Solution ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times with 200 μL of PBS-T and developed using 100 μL/well of o-phenylenediamine dihydrochloride (OPD, Sigma P8287) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times with 300 µl/well of PBS containing 0.05% Tween 20 (PBS-T) (Sigma-Aldrich, USA). Non-specific binding sites were blocked using 300 µl/well of 3% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... External standards were used for concentration determination by comparison of peak retention times (HPLC grade compounds were purchased from Sigma-Aldrich). Peaks were integrated using the Chromaster System Manager Software (Version 2.0 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed three times for 5 minutes with dH2O and transferred to 28% ammonium hydroxide (Sigma-Aldrich, cat# 221228) in dH2O for 30 minutes at RT with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed in 0.3% PBST several times and then incubated with AlexaFluor goat anti rabbit 633 (1:250, Sigma Cat# SAB4600141) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... NC cells were washed three times in Buffer A and incubated in 1 unit/μl of ligase in diluted Ligase Buffer (1:5, Sigma Aldrich) for 30 minutes at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Leukocyte (TRAP) Kit (Sigma). Immunohistochemistry analysis was performed on decalcified paraffin-embedded tissue sections using the HRP/DAB ABC IHC KIT (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Milliplex map kits (Millipore) were used to quantitate serum levels of human and mouse serum cytokines using a Luminex 200 (Luminex ...
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bioRxiv - Biochemistry 2019Quote: ... EasyComp Transformation Kit (Sigma). Transformants were plated onto synthetic complete media and selected by transformation to uracil prototrophy ...
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bioRxiv - Neuroscience 2023Quote: ... ECL-kit (Millipore-WBKLS0500), Bradford reagent (Bio-Rad-500-0006) ...
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bioRxiv - Physiology 2022Quote: ... and glutathione levels were assayed using enzymatic kits (Glutamate Assay Kit, Sigma-Aldrich, MAK004-1KT; α-Ketoglutarate Assay Kit, Sigma-Aldrich, MAK054-1KT ...
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bioRxiv - Genomics 2020Quote: ... The whole slide was then warmed for 1 min at 37°C and immediately fixed for 10 min at room temperature (RT) in a 2% formaldehyde solution (1:20 37% formaldehyde acquired from Sigma-Aldrich, Missouri, USA in 1x PBS pH 7.4). The sections were dried with isopropanol and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Cancer Biology 2021Quote: ... using a cell counting Kit-8 colorimetric assay (CCK-8 kit, Sigma, 96992). Each experiment was repeated three times ...
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bioRxiv - Molecular Biology 2021Quote: ... Trichrome stain (Masson) kit and senescence cells histochemical staining kit (Sigma-Aldrich, Germany); DAB substrate kit (Vector Laboratories ...
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bioRxiv - Physiology 2022Quote: ... and glutathione levels were assayed using enzymatic kits (Glutamate Assay Kit, Sigma-Aldrich, MAK004-1KT ...
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bioRxiv - Microbiology 2023Quote: A colorimetric assay kit (Ammonia Assay Kit AA0100, Sigma-Aldrich, St. Louis, USA) was used to analyze the ammonia content in the samples ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative (q)PCR was performed using SYBR Green Jump Start Taq Ready Mix (Sigma S4438) and Intron-spanning primer pairs (Supplementary Table 5 ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR product were assembled into KpnI and EcoRI difgested pZDonor-AAVS1 Puromycin plasmid (Sigma-Aldrich) and ligated using the Quick Ligase Kit (New England Biolab) ...
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bioRxiv - Microbiology 2019Quote: ... Taq Polymerase and restriction enzymes for cloning and PCR methods were purchased from Sigma Aldrich. PCR cycle was as follows ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were used as a template for PCR using the KOD high fidelity polymerase (Novagen) and a mixture of 8 VH family and IgM-specific primers (listed below ...
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bioRxiv - Genomics 2020Quote: ... Sequences were PCR amplified from zebrafish genomic DNA using the primers in SupplementaryTable16 (Sigma-Aldrich), with the proof-reading iMax ™ II DNA polymerase (INtRON Biotechnology ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned at the NdeI and XhoI restriction sites of pET30a (Novagen) to add a C-terminal 6x-his tag using the Gibson assembly cloning system ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was subsequently cloned into the BamHI-HindIII restriction sites of pETDuet (Novagen) to generate the recombinant plasmid pETDuet-DHS1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The designed promoters were obtained by either PCR (KOD Hot-Start DNA polymerase, Merck-Millipore). PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore) ...
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Developmental Biology 2020Quote: The four lncRNAs tested were PCR-amplified with KOD Xtreme™ DNA Hotstart Polymerase (Millipore) from their 5’ end up until the last codon of the sORF to be tested ...
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bioRxiv - Plant Biology 2021Quote: ... Positive plants were further confirmed by PCR and western blot using GFP antibodies (Sigma-Aldrich). The positive T2 generation transgenic plants were evaluated in a growth chamber.
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... 2 μL of sample was added to 1x PCR Master Mix (Sigma-Aldrich, Basel, Switzerland) and the 4 degenerate primers at a final concentration of 1 μM each in a final volume of 25 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... Clones were screened for N-Se loss by PCR using REDTaq ReadyMix (Sigma, R2523-100RXN) and custom primers (5’-AGCGAGACTCCGTCTAGAAA-3’ ...