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Citations for Millipore Sigma :
3251 - 3300 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Wells were washed four times with PBS-T and 25 μL of prepared Immobilon Western Chemiluminescent HRP Substrate (Millipore #P90719) added to each well immediately prior to the plate being read on a Synergy Neo2 Plate Reader (BioTek Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... and the cells washed three times in PBS and either lysed in 0.1% Triton–X100 (Sigma-Aldrich, St. Louis, MO) for enumeration of cell-associated/adhered bacteria ...
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bioRxiv - Microbiology 2021Quote: ... The ELISA plates were washed four times with PBST and then 100 μl of 2,000-fold diluted anti-strep-HRP (Millipore Sigma) was added into each well for another 1 hr at 37□°C ...
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bioRxiv - Genetics 2019Quote: ... the resin was washed three times in wash buffer (TBS containing 0.1% NP-40 and phosphatase inhibitor cocktails II and III, Sigma-Aldrich). Protein baits were eluted with Strep-elution buffer (2mM desthiobiotin in TBS) ...
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bioRxiv - Genetics 2019Quote: ... Hemocytes were washed (three times, 15 minutes each at 4 °C) in PBS before overnight staining with DAPI 1X (405 nm, Sigma) and Phalloidin 1X (488 nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting multilamellar liposomes were extruded 11 times through two stacked polycarbonate membranes of 0.8 μm pore diameter (Whatman-Nuclepore, Sigma-Aldrich) using a custom-built high-pressure extrusion device or a syringe extruder (Avanti Polar Lipids). ...
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bioRxiv - Microbiology 2022Quote: Apoptosis was induced by rm-CSF1 deprivation (from 30 ng/ml to total removal) for different periods of time or by the addition of 5μg/ml Actinomycin D (AD, #A1410, Sigma-Aldrich) for 16 hours (Lisi et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The coverslips were washed three times with PBS 1X at room temperature and fixed in 1 mL/well of PFA 4% (Sigma) under the chemical hood for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Washing was performed for 5 minutes two times with Washing Buffer A at room temperature followed by incubation with Ligase solution (Sigma) for 30 minutes at 37°C in a humidity chamber ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: Complete GI transit time (GITT) was measured by administering natural carmine red (6%, SigmaAldrich) dissolved in 0.5% methylcellulose (Sigma-Aldrich) by gavage (100ul ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS three times and then incubated in blocking solution (PBS supplemented with 0.1% Triton-X-100 [X100, Sigma-Aldrich] and 10% normal donkey serum [017-000-121 ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The dissociated cells were washed in PBS 3 times and treated with blood cell lysate buffer (Sigma-Aldrich, Cat# R7757).
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bioRxiv - Cell Biology 2022Quote: ... liposomes were extruded with the LipSoFast-Basic extruder (Avestin) 21 times through 2 polycarbonate membranes (Sigma-Aldrich; WHA10417004 or WHA110405). For testing SMA ...
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bioRxiv - Developmental Biology 2022Quote: ... Molten paraffin was then changed five times (each change lasting >1h) before the tissue was transferred into a Peel-A-Way embedding mould (Sigma) for transverse sectioning ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed 4 times with PBS and resuspended in PBS containing 1% BSA and DAPI (1 μg/mL; Sigma) as a nuclear stain ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at 37 °C/5% CO2 and washed three times with 50 µl per well of PBS (Sigma). Immortalised human umbilical vein endothelial cells (Ea.Hy 926 cells ...
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bioRxiv - Genomics 2022Quote: ... The tissue sections were washed three times in PBS-T supplemented with RI (PBS-T+RI) and mounted in 85% ultrapure glycerol (Sigma) supplemented with 2 U/μL RI using #1.5 coverslips (Menzel) ...
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bioRxiv - Microbiology 2022Quote: ... the bacterial suspension was discarded and the tubes were gently washed three times with PBS and stained with 0.1% crystal violet (Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... for the stated times or uninfected were transfected with the desired mammalian expression vector using X-tremeGENE HP DNA transfection reagent (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and were incubated in 200 μL PBS containing 200μg mL-1 MTT (Sigma-Aldrich) in a 96-well culture plate for 2 h at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... washed three times with PBS and cell nuclei were stained with DAPI (1 µg/ml in PBS, Sigma-Aldrich D9542) for 15 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Plant Biology 2022Quote: ... Anti-HA gels were washed 3 times with 20 ml TBS-T and then incubated with peroxidase-conjugated secondary antibody (1:10,000; Sigma A0545) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... dialyzed against PBS (pH 7.2) and concentrated 10 times using centrifugal filtration devices with a 10,000-molecular weight cut-off (Millipore, Darmstadt, Germany). For PCN purification ...
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bioRxiv - Neuroscience 2022Quote: Coronal sections were rinsed and permeabilized three times in 1xPBS with Triton X-100 at 0.5% (Sigma-Aldrich, Madrid, Spain) for 10 minutes each and then blocked in the same solution with 4% of bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Vitrified samples were rinsed four times in pure acetone before infiltration with progressively increasing concentrations of resin (Epoxy embedding medium, Sigma) in acetone while increasing the temperature to 20 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed three times with PBS and permeabilized for 5 min with 0.2% Triton X-100 (Sigma-Aldrich) in 1% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... was washed three times by suspending in purified water and collecting by centrifugation prior to adding 600 mg of purified BSA (Millipore-Sigma) in 6 ml of purified water ...
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bioRxiv - Plant Biology 2024Quote: ... The insoluble pellet was washed five times repeatedly by resuspension in the isolation buffer containing 0.1 g of quartz sand (Sigma-Aldrich) and vortex ...
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bioRxiv - Physiology 2024Quote: ... Pieces were then digested three times for 30 minutes each in 0.7mg/ml Collagenase solution (Sigma-Aldrich, Saint Louis, MO]) at 37°C with agitation ...
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bioRxiv - Neuroscience 2024Quote: Small intestine transit time: A solution containing fluorescein isothiocyanate-dextran (FITC-Dextran, 100 μl; 10 mg/ml in 2% methylcellulose; FD70, Sigma) was administered to mice by gavage through a 21-gauge round-tip feeding needle ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from untreated (0) and UV-C treated WT and jmj27 plants (time points 30 min, 1h and 2h) using Tri-Reagent (Sigma). Reverse transcription reaction (RT ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed in water three times before a 5 min bath of 5% Sodium Thiosulfate (Sigma Aldrich, catalog #S7026).
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bioRxiv - Immunology 2024Quote: ... Fetuin-coated plates were then washed 3 times with PBS-T and incubated for 1 h with a solution of PNA-HRP (cat. # L6135-1MG, Sigma) at 5 μg/ml in conjugate diluent (MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were washed 3 times with 1×PBS and then permeabilized with 0.125% TritonX-100 (Sigma Aldrich, no. 93443) in 1×PBS for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... in a and centrifuged three times at 5000 rpm for 10 min on a Hettlich Universal 320R centrifuge (Sigma-Aldrich). The supernatant was removed ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to the media and cells were harvested at indicated times by washing quickly with 2 ml PBS per well and adding TriPure reagent (Sigma). Cells at time 0 did not receive ActD ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBS-T 0.1% three times and developed with Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500). All blots were imaged via chemiluminescence on a ChemiDoc Touch (cat#12003153 ...
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bioRxiv - Microbiology 2024Quote: ... The compounds were detected by retention times and quantified using calibration curves made with analytical grade standards (Sigma-Aldrich, Germany).
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Systems Biology 2024Quote: ... The complexes were then concentrated by repeated centrifugation and dilution with 1X PBS (normally three times) in Amicon Ultra-4 devices (100K cutoff, Millipore) to reduce the concentration of the 3X FLAG peptide ...
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bioRxiv - Immunology 2024Quote: ... in RPMI 1640 without serum for indicated times and the cell culture medium (supernatant) was collected and filtered using a membrane filter unit (SLGPR33RS, Millipore). Chloroform methanol purification of proteins from the filtered supernatant was performed and dried proteins were eluted with 1× SDS loading sample buffer and boiled at 96□°C for 10□min.
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bioRxiv - Immunology 2024Quote: ... PBMC-containing fraction was then washed two times with 2 mM EDTA in PBS and stored in RNAlater solution (Sigma) at −20° C until RNA extraction ...
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bioRxiv - Immunology 2024Quote: Mast cell cultures were stimulated with the following for the indicated time periods: 1 µg/ml IgE-anti DNP antibody (Sigma) and 0.2 µg/ml HSA-DNP (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... the agarose beads were washed five times in a centrifuge with a solution of 0.1% Tween-20 (Sigma-Aldrich, #P9416).
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bioRxiv - Biochemistry 2023Quote: ... The sample was mixed with about 10 time molar excess of guanosine 5′- [β,γ-imido]triphosphate (GMMPPNP, SIGMA G0635) or guanosine 5’-triphosphate (GTP ...