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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The expression of SNAP-tag fused RBP for each stable cell line was verified by treating with doxycycline (Sigma Aldrich) and SNAP-Cell Oregon Green (NEB).
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bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Developmental Biology 2023Quote: ... 18bp of the reverse complement of the beginning of the DNA tag sequences was added to the 5’ of the reverse primer so that the CRM can be combined with the DNA tag in equal amount using overlap PCR (Xiong et al., 2006) using Expand High Fidelity PLUS PCR (Sigma). The resulting fragment was run on 2% agarose 1x TAE gel ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Plant Biology 2024Quote: ... Expression of proteins was validated by performing a Western blot analysis against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). For the EMSA assays ...
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bioRxiv - Biochemistry 2024Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... The target protein mixed with precision protease and tag was then concentrated with an Amicon Ultra-15 centrifugal filter unit (10,000 MWCO) (EMD Millipore) at 4 ℃ ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Biochemistry 2024Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... and (2′S)-2′-Deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) were purchased from Sigma-Aldrich, dissolved in water ...
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bioRxiv - Cancer Biology 2021Quote: ... along with a photo-initiator 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure2959-410896, Sigma-Aldrich, USA), at a concentration of 0.5 mg/mL followed by exposure to UV-365 for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 97% (v/v) 2’−2’-thio-diethanol (TDE, Sigma, 166782) was treated for clearing ...
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bioRxiv - Developmental Biology 2022Quote: ... 97% (v/v) 2’-2’-thio-diethanol (TDE, Sigma, 166782) in PBS was used for mounting ...
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bioRxiv - Developmental Biology 2021Quote: ... 97% (v/v) 2’−2’-thio-diethanol (TDE, Sigma, 166782) in PBS was used for mounting ...
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bioRxiv - Biochemistry 2021Quote: ... Hsulf-2 (2 µg) digestion with hyaluronidase (Sigma/Aldrich, 2µg) was achieved after a 2 h incubation in 50 mM Tris pH 7.5 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Slit guidance ligand 2 (SLIT-2-N; Sigma, cat#SRP3155), prostaglandin E2 (PGE2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which 2 μl of 2% sodium deoxycholate (Sigma Aldrich) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (Sigma-Aldrich) was peracetylated and administered to semi-confluent bladder cancer cells ...
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bioRxiv - Immunology 2022Quote: ... 2-deoxy-D-glucose (2-DG, 1 mmol/l, Sigma) or diethyl succinate (1 mmol/l ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 μL Protease Inhibitor Cocktail and 2 μL Pefabloc (Sigma) were added before splitting into five aliquots of 400 μL ...
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bioRxiv - Cancer Biology 2019Quote: ... 40 mM 2-chloroacetamide (2-CAA, Sigma, 22790-250G-F), 1X HALT for SILAC samples and lysed with probe sonicator (BRANSONIC) ...
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bioRxiv - Cancer Biology 2021Quote: ... PAI-2 human Plasminogen Activator Inhibitor-2 (SRP3137; Sigma Aldrich); Placenta Growth Factor human (P1588 ...
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bioRxiv - Biophysics 2022Quote: ... and 20 μM 2-β-mercaptoethanol (2-BM) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... 50μM 2-Bromohexadecanoic acid (2-BP) (Sigma-Aldrich, Cat# 238422), 100μM Cycloheximide (CHX ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2-deoxy-D-glucose (2-DG: Sigma, Cat# D8375). The acute effect of BKIDC was assessed by directly adding BKIDC through the injection port ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2-pralidoxime (2-PAM) and paraoxon were purchased from Sigma and stored at room temperature of -20 °C ...
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bioRxiv - Immunology 2023Quote: ... and 2 x 10-5 M 2-mercaptoethanol (Sigma-Aldrich) and used directly in functional experiments.
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bioRxiv - Cell Biology 2023Quote: ... and 1% photo initiator (2-hydroxy-2-methylpropiophenone; Sigma-Aldrich), was introduced between the PDMS stamp and the glass coverslip using capillary action ...
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bioRxiv - Neuroscience 2023Quote: ... or 2-Aminoethyl diphenylborinate (2-APB; Sigma-Aldrich, Catalog #D9754) via 20X spike-in 30 min prior to delivery of Tg or EEAs ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM Ca(NO3)2 ×24H2O (13477-34-4, Sigma), 2.5 mM KH2PO4 (7778-77-0 ...
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bioRxiv - Developmental Biology 2023Quote: Ethosuximide (2-Ethyl-2-methylsuccinimide, E7138, Sigma-Aldrich, Burlington, MA) was similarly stored and prepared (based on methods previously described in (Evason et al. ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2 mM glutamine and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich). MCF10DCIS.com cells (35 ...
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bioRxiv - Microbiology 2022Quote: ... and 2-(acetylamino)-2-deoxy-D-galactose (GalNAc) (Sigma-Aldrich); α-1,2- ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% (v/v) phosphatase inhibitor cocktail 2 (P5726; Sigma Aldrich), and 2% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2-deoxyglucose (2-DG) were purchased from Sigma-Aldrich.
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bioRxiv - Cell Biology 2024Quote: ... apyrase and reactive blue 2 (RB-2) purchased from Sigma. Suramin ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol (CAS 60-24-2, Sigma-Aldrich), and 100 IU/mL recombinant human IL2 ...
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bioRxiv - Biophysics 2024Quote: ... 2 µM porcupine inhibitor IWP-2 (Sigma-Aldrich/Merck, I0536) was added to suppress secretion of endogenous Wnts ...
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bioRxiv - Biophysics 2019Quote: ... his-EmGFP-DBNL(1-256) and his-EmGFP-DBNL(1-374) were diluted to 10 nM and 100 pM concentration in PBS (D8537, Sigma-Aldrich). The experiments were done by putting 30 µl of sample on top of a cleaned glass cover-slip ...
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bioRxiv - Molecular Biology 2021Quote: ... After washing Laemmli buffer 1X was added followed by boiling and loading on SDS-PAGE for visualization by western blotting using anti-His (Clonetech 631212) and anti-GST (Sigma G1160) antibodies.
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Microbiology 2019Quote: ... cells were resuspended in 50 μL stain buffer (FACS buffer containing Qiagen Penta-HIS Alexa Fluor 647 #35370 at a final concentration of 5 μg/mL and Sigma-Aldrich Monoclonal Anti-HA-FITC ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitates were then washed three times with cold Triton lysis buffer and were analyzed by western blot using an anti-His antibody (Sigma Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was then centrifuged at 15000 g for 30 min and applied onto a Ni2+ Hitrap chelating resin (HIS-Select Nickel Affinity Gel; Sigma-Aldrich) equilibrated with 30 mM Tris-HCl pH 7.9 containing 500 mM NaCl (TN buffer ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Systems Biology 2021Quote: ... The pellet was diluted using 250 μL of drop-out medium His− (Yeast Synthetic Drop-out Medium Supplement without Histidine, Sigma Y1751) at 1.92 mg/mL initial concentration ...
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bioRxiv - Microbiology 2020Quote: ... cells were normalized to OD600=0.001 in 1XPBS (phosphate-buffered saline) with 10% of Heat inactivated Fetal Bovine Serum (HI-FBS) (Sigma-Aldrich F9665) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant His-tagged proteins were purified from cell lysates on a nickel-chelating column (Ni-nitrilotriacetic acid agarose; His-select high flow nickel affinity gel; Sigma-Aldrich), as described previously [53] ...