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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Microbiology 2020Quote: ... Samples were clarified by centrifugation and incubated with anti-Myc tag antibody conjugated agarose (Millipore, Cat. no. 16-219) for 3h ...
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bioRxiv - Microbiology 2020Quote: ... was used for SDS-PAGE and Western blotting in Tris-buffered saline and 0.2% Tween 20 (TBST) using Strep-Tag II HRP (1:2,500; EMD Millipore) and rabbit anti-vinculin (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized Bl-Eng2 open reading frame with an upstream enterokinase site and downstream c-Myc and 6X histidine tags was cloned into the SpeI and XhoI sites of the pET43.1b plasmid (Novagen); this forms a fusion protein with an upstream NusA tag ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma, Sigma-Aldrich) followed by secondary GST-purification to remove free GST tag (untagged Mcl-1 collected in flowthrough) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Immunology 2023Quote: ... Flag-tagged CD45 constructs in DP10.7 Jurkat T cell transductants were stained with 10ug/ml anti-Flag-tag polyclonal antibody (Sigma) for 1hr,
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Developmental Biology 2022Quote: TAG determination: 60 μl of each sample were mixed with 60 μl of Triglyceride Reagent (Cat. # T2449, Sigma-Aldrich), incubated at 37 °C for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
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Proteasome granular localization is regulated through mitochondrial respiration and kinase signalingbioRxiv - Cell Biology 2022Quote: ... and 2-[2-(3-chlorophenyl)hydrazinylidene]-propanedinitrile (CCCP) (Sigma), were used at final concentrations of 0.5 μM ...
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bioRxiv - Microbiology 2019Quote: ... 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2019Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Cell Biology 2019Quote: ... Around 2–5 μl of 2 mM levamisole (Sigma) was pipetted out on the center of the agarose pads ...
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bioRxiv - Developmental Biology 2020Quote: ... 616452 (2 μM, Sigma-Aldrich, no. 446859-33-2) and penicillin (100 U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% of photoinitiator (2-hydroxy-2-methylpropiophenone - Sigma) was immediately introduced by capillary action between the PDMS stamp and the glass coverslip ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma-Aldrich, USA), and 4 ng/ml FGF-2 (PeproTech ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2-aminoethoxydiphenyl borate (2-APB; Sigma Aldrich, Oakville, ON) experiments ...
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bioRxiv - Biophysics 2021Quote: ... precut 2×2-well culturewell gasket (GBL103250, Sigma, MO) was attached to the coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... 2% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 500 U/ml universal nuclease ...
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bioRxiv - Immunology 2021Quote: ... plus 2 % FBS and 2 mM EDTA (Sigma-Aldrich) (from now on termed “staining buffer”) ...
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bioRxiv - Immunology 2021Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Biochemistry 2022Quote: ... 2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile (CCCP) (Sigma-Aldrich), rhodamine 123 ...
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bioRxiv - Developmental Biology 2023Quote: ... and 200µM of 2-Mercaptoethanol (2-ME; Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... 2-deoxy-D-glucose (2-DG, Sigma-Aldrich, D8375), D-(+)-mannose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 616452 (2 μM, Sigma-Aldrich, no. 446859-33-2) and penicillin (100 U/mL ...
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bioRxiv - Microbiology 2023Quote: ... ≥95% pure 2’-C-methylcytidine (2’CMC; Sigma, M4949) was serially diluted in 0.5 log10 increments in Milli-Q H2O ...
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bioRxiv - Microbiology 2023Quote: ... Samples were labeled using 2-aminobenzamide (2-AB, Sigma) for 2 hours at 65°C ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human (rh) Interleukin-2 (IL-2, Sigma-Aldrich), rhTNFα (Peprotech ...
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bioRxiv - Biophysics 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB) (Sigma-Aldrich/Merck, Germany), BL-1249 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM dimethyl-2-oxoglutarate (DMG, #349631 Sigma-Aldrich), 500 µM N-acetyl-L-cysteine (NAC ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) was added and the sample was placed on a heating plate at 500 rpm for 10 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... was mixed with 2-Hydroxy-2-methylpropiophenone (Sigma, #405655) at 5%(v/v ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Plant Biology 2019Quote: ... Y187 strains were grown on SD/-Trp-Leu medium and transferred to SD/-Trp-Leu-His medium with 10 mM 3-aminotriazole (3-AT, Sigma) in gradient dilutions (1:10 ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...