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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... coli strains expressing either RFP or GFP were separately cultured in Luria-Bertani (LB) medium (Sigma Aldrich) containing 1 µL/mL of chloramphenicol to retain the recombinant plasmid ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... coli BL21(DE3) strain and protein expression was induced in Overnight Express(tm) Instant TB Medium (Novagen) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli B834(DE3) was grown in chemically defined medium (CDM) supplemented with 20 mg/ml SeMet (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... mice received a single intraperitoneal dose (20 mg/kg) of LPS (Escherichia coli 0111:B4, L2630, Sigma). Mouse bone marrow monocyte derived macrophage (MDMs ...
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bioRxiv - Physiology 2022Quote: ... An additional control group received 2.5 mg/kg BW LPS from Escherichia coli (Sigma, L2630; Deisenhofen, Germany) (n=10 ...
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bioRxiv - Biophysics 2022Quote: ... The ligated pTrcHisB/PRNP construct was used to transform the Escherichia coli host strain BL21(DE3) (Novagen), genotype F′ ompT hsdSB (rB-mB- ...
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bioRxiv - Microbiology 2024Quote: All Escherichia coli strains used in this thesis were grown aerobically in Luria-Bertani (LB) broth (Sigma). E ...
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bioRxiv - Microbiology 2024Quote: ... coli culture at an optical density at 600 nm (OD600) = 0.84 1:2,000 in isotone (Sigma Aldrich) and measuring 75 µL thereof at a Coulter counter (Multisizer 4e (Beckman Coulter)) ...
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bioRxiv - Molecular Biology 2023Quote: ... mice were injected with 2mg/kg body weight phenol-extracted LPS (Escherichia coli O26:B6, Sigma-Aldrich) dissolved in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Both plasmids (ATAD2/B) were transformed into Escherichia coli BL21(DE3) pLysS competent cells (Novagen, MA, USA) for protein expression.
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bioRxiv - Evolutionary Biology 2023Quote: ... coli clones were grown at 37°C under aeration in liquid media Lysogeny Broth (LB) from SIGMA — or lactose supplemented McConkey agar plates and LB agar plates ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... coli expression and driven by a T7 promoter and lac operator in a pET28a expression vector (Novagen). Specifically ...
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bioRxiv - Neuroscience 2023Quote: ... adult C57Bl/6 mice were treated intra-peritoneally (IP) with LPS (Escherichia coli O55:B5, Sigma, L2880) at 5 mg/kg and brains were collected 3h (n=5 ...
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bioRxiv - Biochemistry 2023Quote: ... coli and synthesized by Geneart (http://www.geneart.com).The genes were then sub-cloned into pET28a vector (Novagen) using the restriction sites NdeI and XhoI with an N-terminal His-tag in frame.
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bioRxiv - Microbiology 2023Quote: ... coli strains was performed at 37 °C in LB (20g/L LB Broth (Lennox) (Sigma-Aldrich; L3022), 2xYT (tryptone 16g/L (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... coli ST18 was grown in LB medium supplemented with 50 μg/ml 5-aminolevulinic acid (Sigma-Aldrich) at 37 °C to an OD600 of 0.3 ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized by intraperitoneal injection with Avertin (2, 2, 2-Tribromoethanol, Sigma-Aldrich catalog # T4). Cardiac perfusion was performed with 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... and 2 mM Trolox (6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid, 238813, Sigma) was flowed into chamber and then mounted onto microscope stage for smFRET observations ...
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bioRxiv - Developmental Biology 2021Quote: ... 250–1000 embryos were collected and transferred into a Petridish containing Pronase E protease solution (Sigma-Aldrich, P5147-1G). When 20–30% embryos were hatched ...
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bioRxiv - Neuroscience 2021Quote: Cre-mediated recombination was induced via intraperitoneal injection of 4-hydroxytamoxifen (50:50 E and Z isomers, Sigma-Aldrich) dissolved in sunflower seed oil ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl cold lysisg/μl cold lysisl U6.3>Lhx5/Sox1/Six3 gRNA f+e was microinjected together with the Fast Green solution (Sigma) at a 2:1 ratio to ease the detection of the injection site of the developing neural tube and eye respectively with the help of borosilicate glass capillaries and electroporated as previously reported86–88 using the Intracel TSS20 OVODYNE Electroporator ...
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bioRxiv - Genomics 2020Quote: ... Cells were released from G1 by washing with YPD containing auxin and 0.15 mg/mL pronase E (Sigma Aldrich) three times ...
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bioRxiv - Neuroscience 2021Quote: ... 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH 58261, 50nM) were purchased from Sigma (St. Louis, MO). CGS 21680 and SCH 58261 were made up into a 5 mM stock solution in dimethylsulfoxide (DMSO) ...
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bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
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bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin knockdown was induced in shE-cadherin cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
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bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Cancer Biology 2022Quote: Sirt1 conditional knockout E-NOTCH1-induced T-ALL-bearing mice were treated with vehicle only (corn oil; Sigma, C8267) or tamoxifen (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... Ice-cold acetone-fixed cryostat sections (10μm) were prepared and stained with hematoxylin and eosin(H&E) (Sigma-Aldrich) using routine methods ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were supplemented with IPH50 buffer and protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), centrifuged at 15,000rpm for 20 minutes ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were collected by filtration and promptly washed twice on the filter using one culture volume of H2O and then resuspended into YPD medium containing 0.2 mg/mL pronase E (Sigma).
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bioRxiv - Bioengineering 2022Quote: ... samples were stained with hematoxylin and eosin (H&E) through a 5- minute incubation in Harris Hematoxylin (Sigma Aldrich), followed by a wash in tap water and three dips in acid ethanol to destain ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... These sections were stained with Hematoxylin & Eosin (H&E) and periodic acid-Schiff (PAS) according to the manufacturer’s protocol (Sigma). The muscle sections were analyzed blindly by a pathologist ...
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bioRxiv - Immunology 2023Quote: Camostat (cat. no. SML0057) and E-64d (cat. no. E8640) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A short synthetic dsRNA ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Bioengineering 2024Quote: ... 1L of perfusate was composed of 950 mL of William’s E hepatocyte culture media (Sigma Aldrich, Burlington, MA, USA) to supply the graft with amino acids and nutrition during perfusion ...
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bioRxiv - Neuroscience 2023Quote: ... The final concentrations of drugs used in the study: 10 μM of E-64 (Sigma-Aldrich, Cat.: #E3132-1MG), 60 nM of t-NED19 (Bio-techne ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were centrifuged at 14,000 rpm for 15 minutes and supernatant mixed with T7 Tag Antibody Agarose (EMD Millipore) for immunoprecipitation or NeutrAvidin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... DNA encoding the POTRA domains (1-4) of BamA with a C-terminal strep-tag (but without the signal sequence) was cloned in pET21a (Novagen). All the proteins above were overexpressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...