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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... were treated with 1 μg/ml LPS from Escherichia coli (serotype 0111: B4, Sigma-Aldrich, St. Louis, MO, USA) and 100 ng/ml IFN-γ (ProSpec-Tany Technogene Ltd ...
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Molecular Biology 2021Quote: Wild-type or mutated pMAL-c2x-malE-L375-his10 plasmids were transformed into Escherichia coli strain BL21 (EMD Millipore) for subsequent growth in LB broth supplemented with 50 μg/ml carbenicillin and 0.2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... Maturation of iDCs was performed in the presence of 200 ng/ml LPS from Escherichia coli (Sigma Aldrich, Germany) and 10µg/ml myelin basic protein (MBP ...
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bioRxiv - Immunology 2022Quote: ... CTV labeled cells at a concentration of 2×106 cells per mL were stimulated in complete B cell medium supplemented with combinations of 10μg/mL of LPS from Escherichia coli O55:B5 (Sigma) or 1μg/mL CD40L (Peprotech) ...
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bioRxiv - Microbiology 2023Quote: Atypical Enteropathogenic Escherichia coli O125ac:H6 (DSM8711) was purchased from the Leibniz Institute DSMZ and cultured in Luria-Bertani (LB) medium (Merck Millipore) and when necessary supplemented with kanamycin (50 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... coli Tuner (DE3) cells and purified by immobilized metal affinity chromatography (IMAC) using PureProteome™ Nickel Magnetic Beads (Millipore). For a detailed protocol ...
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bioRxiv - Molecular Biology 2023Quote: All PhPINK1 (residues 115–575) constructs and λ-PP were expressed in Escherichia coli Rosetta2 (DE3) pLacI cells (Novagen) and purified as described previously (17) ...
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bioRxiv - Immunology 2023Quote: ... Ultrasound-guided intra-amniotic injection of lipopolysaccharide of Escherichia coli O111:B4 (LPS; Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 100 ng dissolved in 25 µL of sterile 1X PBS was performed into each amniotic sac using a 30 G needle (BD PrecisionGlide Needle) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were were cultivated in Lysogeny Broth (Bertani, 1951) medium supplemented with 100 mg/L of ampicillin (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... 3- to 5-month-old male C57Bl/6J received a single intraperitoneal (i.p.) injection of lipopolysaccharide (1.5 x 107 EU/kg) from Escherichia coli 0111:B4 (Sigma, #L2630) or an equivalent volume of sterile saline ...
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bioRxiv - Immunology 2023Quote: ... background were injected intraperitoneally (i.p.) with 35 mg/kg of body weight LPS (lipopolysaccharides from Escherichia coli O111:B4, obtained from Sigma, catalog number ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured in the presence of brefeldin A and LPS (1 μg/ml Escherichia coli O55:B5; Sigma). For intracellular cytokine staining ...
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bioRxiv - Plant Biology 2020Quote: ... 2015) e cleaned using stage-tips preparations with 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction following the manufacturers instructions.
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bioRxiv - Developmental Biology 2019Quote: Embryos designated for hematoxylin and eosin (H&E) staining were dissected and fixed overnight in 10% neutral buffered formalin solution (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Cancer Biology 2021Quote: ... After 10 days constructs were fixed in 4% paraformaldehyde and embedded in paraffin and stained with H&E and stained for Fibronectin with FN1 antibody (F3648, Sigma Aldrich). For each construct the number of cells invading into the collagen was counted in at least five different fields of view under light microscopy ...
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bioRxiv - Biochemistry 2021Quote: ... 0.01 mg aprotinin and 0.1 mM trans-epoxysuccinyl-L-leucyl amido (4-guanidino) butane (E-64) as a protease inhibitors (Sigma Aldrich®) and lysed by sonication (5 pulses for 1 min each ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Microbiology 2021Quote: ... PMH were cultured in hepatocyte culture medium (William’s E medium, GlutaMAX™ Supplement, Gibco™ 32551020; 100 U/ml penicillin/streptomycin, Sigma P4333 ...
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bioRxiv - Cancer Biology 2021Quote: ... The retrieved cells were seeded on collagen-treated vessels (at a density of 150,000 viable cells per cm2) and maintained in Williams’ medium E (Sigma, Darmstadt, Germany) supplemented with 1 μM dexamethasone ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with 1 ml of SF DMEM containing the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 μM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A)(SIGMA, P1860) in the presence or absence (control ...
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bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... As negative control one set of samples was pre-incubated with 20 μM E-64 (Sigma-Aldrich, St. louis, Mississippi, USA). Subsequently ...
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Developmental Biology 2024Quote: ... the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 µM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A, 1/1000)(SIGMA, P1860) with or without 20 nM Wnt5a in the presence or absence of 500 nM RAP for 3 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: Cells post aggregation and appropriate control (EGTA for E-cad and Cells without DNA labelling for DNA-cad) were incubated on ice with RIPA lysis buffer (Sigma Aldrich) supplemented with proteases and phosphatases inhibitor cockbTails (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Central transverse sections of the heart were cut into 4 μm sections and stained with haematoxylin and eosin (H&E) and Masson’s Trichrome staining (#HT15, Sigma-Aldrich, UK) for collagen detection ...
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bioRxiv - Immunology 2023Quote: ... Staining was done using hematoxylin and eosin (H&E) to view the alveolar spaces and inflammatory cells and Grocott’s Methenamine Silver stain (GMS) (Sigma-Aldrich, USA) to stain fungal cells in infected tissue ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated with primary antibodies (CK8, Abcam ab182875,1:200. CK14, Biolegend 905301,1:400; E-cadherin, Biolegend 147301,1:200; α-SMA, Sigma A5228, 1:500) diluted in blocking buffer at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 4a-e, 4g, 4j, 5d-f) were cultured in 24-well plates (Falcon, 353047) on mixed cellulose ester Millicell inserts (Millipore, PIHA01250). Cells were plated at 100,000 cells/insert and cultured 6-8 weeks before experimental manipulations ...
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bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... respectively) were seeded in Seahorse XF 96 designated plates using 100 µg/ml poly-D-lysine (Sigma-Aldrich, A-003-E) and assayed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of AsCas12a nuclease in the stable HEK 293 T-REx cell line was induced by 100 ng/mL tetracycline for 24 h and assayed by Western immunoblotting using FLAG-tag specific antibodies (Sigma-Aldrich, F1804).
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bioRxiv - Immunology 2022Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30uM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60uM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and NURF-1.F isoform with FLAG tag was detected using 1:1000 Millipore ANTI-FLAG antibody (Millipore Sigma, Catalog number: F3165).
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801; HA: Roche #11867423001; VSVG: Sigma #SAB4200695) in a total volume of 500 μL 50 mM MES (pH 5.0) ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used: DYKDDDDK Tag Antibody (D6W5B rabbit mAb #14793; Cell Signaling Technology; it binds to the same epitope as Sigma’s Anti-FLAG M2 Antibody ...
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bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Plant Biology 2021Quote: ... LHCBM9 (M. Schroda, unpublished data), CF1β (Lemaire and Wollman, 1989), RPL1 (Ries et al., 2017) and the HA-tag (Sigma-Aldrich H3663). Anti-rabbit-HRP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... For Flag-tag immunoprecipitation samples were incubated with 100 µl of anti-FLAG M2 magnetic beads (#M8823, Sigma-Aldrich Chemie; Munich, Germany) for 4 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...