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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Cell Biology 2019Quote: ... IP or His-PD Samples were resolved by SDS-PAGE (10-15% gels) and transferred to Immobilon-P membranes (Millipore Inc.), which were then immunoblotted with the following antibodies against ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was separated on a 10% SDS-PAGE gel and further analyzed by immunoblotting using anti-His and anti-GST antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were mixed as indicated in the figure and rotated with either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare ...
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bioRxiv - Physiology 2020Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... cells were normalized to OD600=0.001 in 1x PBS (phosphate-buffered saline) with 10% of Heat inactivated Fetal Bovine Serum (HI-FBS) (Sigma-Aldrich F9665) and incubated at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Immunology 2022Quote: ... To prepare HI-WCA-OT-109 copies/ml of bacterial suspension was made up in R10 media: RPMI 1640 (Sigma, R0883) containing 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Plant Biology 2023Quote: ... All ATG3 recombinant proteins (wild-type and Cys-to-Ser mutants) were purified by affinity chromatography on a His-Select Nickel Affinity Gel (Sigma, P6611) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 10% input aliquots were taken and the rest of the sample was incubated with HIS-Select® Nickel Magnetic Agarose Beads (Sigma) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... was concentrated from the flow-through of the second passage through the His-trap column using an Amicon® Ultra Centrifugal Filter (Millipore) with a 30 kDa cut-off ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... 293FT were exposed to supernatants containing pMXs-IRES-Blast-STING-His retroviruses and 4 μg/mL polybrene (Millipore, TR-1003-G). Two days post transduction ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Biochemistry 2024Quote: ... Ubiquitin was expressed as an untagged protein and then purified and labelled with Cy3 as per ref53.TRIB1 C-terminus (345-372) was first purified using Ni2+ affinity resin (His-Select resin (Sigma-Aldrich) before being dialysed into PBS pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... placed in digestion media containing 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 2% PenStrepNeo (Sigma), 0.02 mg/mL insulin (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% Hemicellulase (Sigma), 1% driselase (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 2% raffinose (Sigma) or 1% raffinose/2% galactose (AppliChem ...
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bioRxiv - Cell Biology 2019Quote: ... Binucleine-2 (Sigma) was used to acutely inhibit Aurora B activity in Drosophila S2 cells at a final concentration of 40 μM in all experiments ...
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bioRxiv - Genetics 2019Quote: ... 2% polyvinylpyrrolidone (Sigma), 2 mM L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... 2 % FBS (Sigma), 0.1% Sodium Azide (Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... 2] octane (Sigma) and 50 or 80% glycerol in 50 mM Tris-HCl (pH 8.0).
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bioRxiv - Microbiology 2019Quote: ... Pitstop-2 (Sigma), CK-869 (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 2×SSC (Sigma), 10 % formamide (Calbiochem) ...
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bioRxiv - Cell Biology 2020Quote: ... Prohibitin 2 (Sigma Rieske (Rabbit serum ...
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bioRxiv - Microbiology 2022Quote: ... 2 (Sigma-Aldrich) plates supplemented with 5% defibrinated sheep’s blood at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2022Quote: ... CMTI-2 (Millipore), were electroporated using the Nucleofector II (Lonza) ...
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bioRxiv - Neuroscience 2022Quote: ... 2% PFA (Sigma), 0.1M sodium cacodylate trihydrate ...
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bioRxiv - Microbiology 2020Quote: ... the plates were emptied and a volume of 300 μl/well of blocking buffer (PBS-0.1% Tween (Sigma)-2% BSA (Sigma)) was added ...
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bioRxiv - Microbiology 2020Quote: ... 2% nystatin (Sigma) and 0.1% penicillin-streptomycin (Gibco® ...
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bioRxiv - Neuroscience 2021Quote: ... 2-mercaptoethanol (Sigma) and ESGRO leukemia inhibitory factor (LIF ...
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bioRxiv - Bioengineering 2019Quote: ... 2% FBS (Sigma), 10 µg/mL insulin (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... washed with PBS and blocked in blocking buffer (0.05% Tween (Sigma)/ 2% BSA (Sigma) in PBS ...
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bioRxiv - Immunology 2020Quote: ... the plates were emptied and a volume of 300 μl/well of blocking buffer (PBS-0.1% Tween (Sigma)-2% BSA (Sigma)) was added ...
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Myofiber injury induces capillary disruption and regeneration of disorganized microvascular networksbioRxiv - Physiology 2021Quote: ... 2 CaCl2 (Sigma), 1.17 MgSO4 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... / 2% Acrylamide (Sigma) in PBS and incubated for 5 h at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 2-mercaptoethanol (Sigma), insulin (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% NaN3 (Sigma) was dissolved in NB media ...
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bioRxiv - Neuroscience 2020Quote: ... Lidocaine (2%) (Sigma) and CNQX disodium salt (100 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... / 2% Ammonia (Sigma). After vacuum centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... Lidocaine (Sigma, 2%) was infused into the olfactory peduncle at the rate of 0.25 uL/min using a syringe pump ...
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bioRxiv - Physiology 2022Quote: ... 2 CaCl2 (Sigma), 1.17 MgSO4 (Sigma) ...
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bioRxiv - Physiology 2022Quote: Metric 2 (Sigma). The standard deviation of TGF oscillations ...
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bioRxiv - Cell Biology 2022Quote: ... 2-deoxyglucose (Sigma/Merck ...