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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Cells were released from G1 by washing with YPD containing auxin (if indicated) and 0.15mg/mL pronase E (Sigma Aldrich) three times ...
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bioRxiv - Microbiology 2021Quote: ... was incubated in 50 mM Tris-acetate buffer (pH 7.4) containing 5 mg/mL protease mixture (Pronase E, Sigma-Aldrich) in a total volume of 1 mL at 28 °C for 2 h and then directly resuspended in the standard SDS sample buffer before SDS-PAGE and western blot analysis.
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bioRxiv - Biochemistry 2021Quote: ... 0.3 ml of media were precipitated with 25 µl EZ view Red anti-HA affinity gel (E 6779; Sigma-Aldrich) according to the manufacturers’ protocol ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae in Colombia blood agar (E&O Laboratories, UK) followed by Todd-Hewitt broth plus 0·5% yeast extract (Sigma), both at 37°C in 5% CO2 ...
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Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... animal cap - (E) vegetal cap. Total RNA (X. laevis and A. mexicanum) was extracted using TriReagent extraction and LiCl precipitation (Sigma) (details in (Sindelka et al ...
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bioRxiv - Cell Biology 2022Quote: ... 80-mer oligonucleotides biotinylated either on the 5’ or 3’ end containing the E-box of the Lgr5 promoter or a mutated E-box (see table) were annealed with the corresponding antisense sequence and bound to streptavidin agarose beads (S1638, Millipore). Beads were washed 3 times ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in a T75 flask (pre-coated with 50 μg/mL poly-D-lysine (Merck Millipore, A-003-E) for 1 hour) ...
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bioRxiv - Genetics 2024Quote: ... in TC-treated 12- or 6well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E). At 3-4 DIV ...
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bioRxiv - Neuroscience 2023Quote: ... and the gamma secretases inhibitor was purchased as Compound E (γ-Secretase Inhibitor XXI) from EMD Millipore (Burlington, MA, USA). Ammonium acetate (Cat# A11450 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with hematoxylin and eosin (H&E) and for Tph1 with an anti-Tph1 primary antibody (Sigma; #T0678). Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
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bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
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bioRxiv - Cell Biology 2023Quote: Transient knockdown of S100A11 and E-cadherin in MCF-10A cells was performed by transfection of MISSION® Predesigned (Sigma) or custom-made siRNAs ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 μm sections were taken before being baked for 3 hours at 60°C and stained with hematoxylin and eosin (H&E, Sigma) or Masson’s trichrome (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
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bioRxiv - Immunology 2023Quote: ... HEK293FT (provided by T. Brdicka, IMG) and MC-38 cells (provided by E. Palmer, University Hospital Basel) were cultivated in DMEM media (Sigma). The media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: DO11.10 cell line (provided by Tomas Brdicka, IMG) and T2–Kb (provided by E. Palmer, University Hospital Basel) were cultivated in RPMI media (Sigma). HEK293FT (provided by T ...
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bioRxiv - Bioengineering 2023Quote: ... samples were mounted on paraffin blocks and sectioned into 5 µm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR ...
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bioRxiv - Neuroscience 2024Quote: ... The first injection of pre-treatments was a solution of ethosuximide (150 mg/kg of 84 mg/ml in phosphate buffered saline, s.c.; Cat# E;7138, Sigma-Aldrich). Ethosuximide was used because the background strain ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and MRS 1220 (N-(9-chloro-2-furan-2-yl-[1,2,4]triazolo[1,5-c]quinazolin-5-yl)-2-phenylacetamide) were purchased from Sigma-Aldrich and dissolved in dimethyl-sulphoxide (DMSO) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: The peptides mUNO (sequence: CSPGAK-COOH) and FAM-mUNO (FAM-Ahx-CSPGAK-COOH) were purchased from TAG Copenhagen and doxorubicin (DOX) from Sigma-Aldrich. St-PGA was kindly provided by Polypeptide Therapeutic Solution S.L ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Biochemistry 2020Quote: ... Western blot analysis was used to verify the identity of PfAdoMetDC using monoclonal α-Strep-tag II antibodies (Novagen, Wisconsin, USA). Following Strep-Tactin Sepharose affinity column purification ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Immunology 2022Quote: ... followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215, Sigma-Aldrich), γ-catenin (sc-514115 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (Sigma-Aldrich) to detect CBUA0027 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck ...
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bioRxiv - Cell Biology 2022Quote: ... oligos carrying the ALFA tag sequence codon optimized for yeast and flanked by SalI and BglII sites were synthesized by Sigma-Aldrich. These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (EMD Millipore) to detect CBUA0027 ...
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Immunology 2023Quote: ... supplemented with Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30μM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60μM ...
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bioRxiv - Bioengineering 2023Quote: A 10 mg/mL suspension of TAG drops was prepared by dissolving 1 mg of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.) in 100 μL of a chloroform–methanol solution (2:1) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Biochemistry 2023Quote: ... were cloned into pHAT4 (Peranen et al., 1996) for expression as a TEV-cleavable His6-tag fusion in BL21 (DE3) cells (Novagen®) in 2xYT media ...
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bioRxiv - Biochemistry 2023Quote: ... and BRCA2 Ex14-AAA were cloned into pMAT11 for expression as TEV-cleavable His6-MBP-tag fusions in BL21 (DE3) cells (Novagen®) in 2xYT media ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were spun down at 10,000g for 10 minutes at 4C and the supernatant was used for immunoprecipitation using an antibody against HA-tag (Sigma, H3663) with Dynabeads protein G (Invitrogen ...