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Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Neuroscience 2020Quote: ... in 5 mL of 2-methyl-2-butanol (Sigma Aldrich, #240486). Mice were deeply anesthetized using 15 µL of Avertin 2.5% per gram of body weight ...
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bioRxiv - Bioengineering 2021Quote: ... (2) hexafluorophosphate azabenzotriazole tetramethyl uronium (HATU; 2 equiv:ELP amine, Sigma, 445460), and (3 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM penicillin/streptomycin and 2 mM L-glutamine (Sigma, UK). Hek-293T cells were plated in 24 well plates for 24 hours before transduction with LV-LTα or LV-GFP at MOI 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μM ponatinib (Selleckchem) or 2 μM iodoacetamide (IAA) (Sigma-Aldrich), the other half was treated with vehicle (DMSO or water ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg/mL 4′,6-Diamidin-2-phenylindol (DAPI, Sigma-Aldrich) in 1X PBS was added for 2 min followed by a final washing step ...
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bioRxiv - Microbiology 2020Quote: ... 2% glycerol or 2% casamino acids and in RPMI-1640 (Sigma, containing L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... E2 and P4 were solubilized in 2-butanol (2-BtOH; Sigma) as our previous studies demonstrate that this reagent does not interfere with NgPLD activity ...
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bioRxiv - Biochemistry 2019Quote: 2 µl of 1 mM 2’,7’ Dichlorofluorescein diacetate (Sigma-Aldrich) dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one ...
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bioRxiv - Immunology 2019Quote: ... and recombinant human interleukin-2 (rhIL-2; 25U/ml; Sigma-Aldrich). To mimic a transient stimulation ...
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bioRxiv - Biochemistry 2019Quote: ... Released glycans were labelled with 2-aminobenzamide (2-AB; Sigma-Aldrich) and purified on Whatman 3 mm chromatography paper ...
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bioRxiv - Bioengineering 2020Quote: ... The photoinitiator (2-hydroxy-2-methylpropiophenone, Darocur 1173, 405655, Sigma-Aldrich) is introduced with the two precursors.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25mM Hepes (N-[2-hydroxyethyl]piperazine-N0-[2-ethanesulfonic acid; Sigma]), supplemented with 10% heat inactivated FBS (GIBCO ...
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bioRxiv - Molecular Biology 2019Quote: ... or MG132 (10 μM, 2 h, replicates 2, 3, 4; Sigma). Proteins were precipitated in acetone ...
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bioRxiv - Immunology 2020Quote: 2-deoxy-d-glucose (2-DG; Sigma-Aldrich, St. Louis, MO) treatment of wounded zebrafish larvae (simple tail transection ...
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bioRxiv - Neuroscience 2022Quote: 2-Bromopalmitate (or 2-bromohexadecanoic acid, Sigma-Aldrich, Cat no. 238422) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... 2% pectone (Difco) and 2% glucose (Sigma-Aldrich, Carlsbad, CA, USA) or selective medium YNB containing ...
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bioRxiv - Synthetic Biology 2022Quote: ... was mixed with photo-initiator 2-hydroxy-2-methylpropio-phenone (Sigma) (90/10% w/w) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline and 2 µM Ara-C (Sigma-Aldrich). From day 3 – 6 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mm samples were embedded instead in 2% agarose (Sigma-Aldrich) in 0.15 M CaC or in water (depending on the last staining step that the sample was exposed to ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (Sigma H0887), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma P4333 ...
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bioRxiv - Cell Biology 2024Quote: ... and drugs (2-deoxy-d-glucose (2-DG, SIGMA, 1 mM), N-acetylcysteine (NAC ...
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bioRxiv - Microbiology 2024Quote: ... and 2 μM ISG65 or 2 μM bovine serum albumin (Sigma) were combined in phosphate buffered saline pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 mM 2-oxoglutarate (2-OG, Sigma-Aldrich, St. Louis, Missouri), pH 8.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... or 2-Deoxy-D-Glucose (2-DG; D8375; Sigma; 10 mM), as stated.
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bioRxiv - Immunology 2022Quote: ... followed by clearing/mounting using 2’-2’-thio-diethanol (Sigma, 166782) as previously described6 ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM potassium hexacyanoferrate(II) trihydrate (C6FeK3N6+2 · 3H2O; Sigma Aldrich), 2 mM potassium hexacyanoferrate(III ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2-deoxy-D-glucose (2-DG) (50 mM) (Sigma Aldrich). When stated ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2% FBS and 2% antibiotic antimycotic solution (Sigma-Aldrich).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: Mice aged 6 months or naked mole-rats aged 2-4 years were intraperitoneally (i.p.) injected with 1mg (2′S)-2′-Deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU; Sigma) from a 10mg/ml stock in DMSO diluted with sterile 0.9% sodium chloride solution (Sigma).
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bioRxiv - Neuroscience 2022Quote: Mice were deeply anesthetized with 1 mL (overdose) of Avertin (2, 2, 2-Tribromoethanol; Sigma Aldrich, 20 mg/mL) and perfused intracardially with 12 mL of iced PBS ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Developmental Biology 2021Quote: ... One group was injected intraperitoneally (i.p.) with LPS (Escherichia coli serotype 0111:B4; Sigma-Aldrich Canada, Oakville, ON, Canada) on GD13.5 (10 µg/kg ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2020Quote: ... β- and γ-Syn were produced by co-expressing PT7-7 plasmids with yeast N-acetyltransferase complex B (NatB)74 in Escherichia coli BL21 Star (DE3) cells using M9 minimal media supplemented with 0.5 g/L of 15NH4Cl (Sigma). Protein purification under non-denaturing conditions was performed as described previously75 ...
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bioRxiv - Immunology 2021Quote: ... Phorbol 12-myristate 13-acetate (P8139) and Lipopolysaccharides from Escherichia coli 055:B5 (L2880) were purchased from Sigma-Aldrich. Pam3CSK4 (tlrl-pms) ...
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bioRxiv - Biophysics 2020Quote: ... coli DH10BacY competent cells and plated on LB-Agar plates supplemented with 30 µg ml-1 kanamycin (#K4000, Sigma), 7 µg ml-1 gentamycin (#G1372 ...
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of LPS from Escherichia coli O111:B4 (sigma) in 0.5% solution of carboxymethyl cellulose (sodium salt; Sigma) was injected into the air pouch ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... coli OP50-1 (from the Caenorhabditis Genetics Center, CGC) which was grown in standard LB broth with Streptomycin (Sigma) from a 12.5 mg/ml stock dissolved in H20 ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Bioengineering 2020Quote: Experiments to confirm the functionality of the vasculature channel and its ability to respond to stimuli were conducted by exposing the epithelial channel of the Open-Top Organ-Chip to 50μl of 10μg/ml Lipopolysaccharide (LPS)(Escherichia coli O111:B4, Sigma L4391) solution for 8 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... coli genes for proteins bS6 and bL9 were PCR-amplified and cloned individually into the pET28(a) plasmid (Novagen). Recombinant single-cysteine proteins were then expressed ...